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Invitrogen
Blooms Syndrome Polyclonal Antibody
FIGURE: 1 / 11
Blooms Syndrome Antibody (PA5-27384) in ICC/IF
Immunofluorescence analysis of Blooms Syndrome was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Blooms Syndrome Polyclonal Antibody (Product # PA5-27384) at 5µg/mL in 0.1% BSA, incubated at 4 degree Cel... View More
Product Details
PA5-27384
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Fruit fly
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1235 and 1417 of Human BLM
Conjugate
Form
Liquid
Concentration
1.34 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2544860
Product Specific Information
Recommended positive controls: Jurkat, Raji, K562, NCI-H929, NIH-3T3, JC, BCL-1.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Bloom syndrome protein; Bloom syndrome, RecQ helicase-like; DNA 3'-5' helicase BLM; DNA helicase, RecQ-like type 2; MGC126616; MGC131618; MGC131620; RecQ protein-like 3; RecQ-like DNA helicase BLM; RecQ2
Gene Aliases: BLM; BS; RECQ2; RECQL2; RECQL3
UniProt ID: (Human) P54132
Entrez Gene ID: (Human) 641
bubble DNA binding
p53 binding
single-stranded DNA binding
ATP-dependent DNA helicase activity
helicase activity
protein binding
ATP binding
ATP-dependent helicase activity
four-way junction helicase activity
ATPase activity
annealing helicase activity
ATP-dependent 3'-5' DNA helicase activity
G-quadruplex DNA binding
DNA helicase
regulation of cyclin-dependent protein serine/threonine kinase activity
double-strand break repair via homologous recombination
DNA double-strand break processing
DNA synthesis involved in DNA repair
strand displacement
DNA strand renaturation
DNA replication
DNA repair
DNA recombination
cellular response to DNA damage stimulus
mitotic G2 DNA damage checkpoint
response to X-ray
protein sumoylation
replication fork processing
DNA duplex unwinding
positive regulation of transcription, DNA-templated
negative regulation of DNA recombination
replication fork protection
protein oligomerization
negative regulation of cell division
cellular response to ionizing radiation
cellular response to hydroxyurea
cellular response to camptothecin
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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