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CACNB2 Antibody (MA5-45451) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with Cav beta 2 monoclonal antibody (Product # MA5-45451) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav beta 2 antibody (D) Compo... View More
产品信息
MA5-45451
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
N8b/1
抗原
Synthetic peptide amino acids 189-205 of rat CavBeta2
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
内含物
no preservative
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931905
产品详细信息
1 µg/mL of MA5-45451 was sufficient for detection of Cav beta2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody|Detects approximately 78 kDa. No cross reactivity against Cav beta 1, Cav beta 3, Cav beta 4.
靶标信息
The expression of multiple classes of voltage dependent calcium channels (VDCCs) allows neurons to tailor calcium signaling to functionally discrete cellular regions. Although N-Type VDCCs exist before birth which is consistent with a role in migration, most N-Type VDCCs subunit expression is postnatal and is important in the genesis of synaptic transmission in discrete hippocampal fields.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CAB1; CAB2; CACNLB1; Calcium channel voltage-dependent subunit beta 2; calcium channel, voltage-dependent, beta 2 subunit; CCHLB1; Lambert-Eaton myasthenic syndrome antigen B; MGC138502; MGC138504; MGC41896; myasthenic (Lambert-Eaton) syndrome antigen B; MYSB; Voltage-dependent L-type calcium channel subunit beta-2; voltage-gated calcium channel beta2 subunit transcript variant X13
基因别名: AW060387; CAB2; CACNB2; CACNLB2; CAVB2; Cavbeta2; Cchb2; MYSB
UniProt ID: (Human) Q08289, (Mouse) Q8CC27, (Rat) Q8VGC3
Entrez Gene ID: (Human) 783, (Mouse) 12296, (Rat) 116600
voltage-gated calcium channel activity
calcium channel regulator activity
calcium channel activity
protein binding
high voltage-gated calcium channel activity
protein kinase binding
protein domain specific binding
actin filament binding
phosphoprotein binding
voltage-gated calcium channel activity involved in cardiac muscle cell action potential
voltage-gated calcium channel activity involved in AV node cell action potential
voltage-gated ion channel activity
voltage-gated ion channel
chemical synaptic transmission
neuromuscular junction development
visual perception
positive regulation of calcium ion transport
cardiac conduction
calcium ion import
calcium ion transmembrane transport
membrane depolarization during AV node cell action potential
regulation of heart rate by cardiac conduction
establishment of protein localization to plasma membrane
membrane depolarization during atrial cardiac muscle cell action potential
regulation of voltage-gated calcium channel activity
positive regulation of high voltage-gated calcium channel activity
positive regulation of calcium ion transmembrane transport via high voltage-gated calcium channel
transport
ion transport
calcium ion transport
regulation of ion transmembrane transport
