Invitrogen

CASK Polyclonal Antibody

CASK Antibody in Immunocytochemistry (ICC/IF)

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CASK Antibody (PA1-544) in ICC/IF

Immunofluorescent analysis of CASK was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CASK Rabbit Polyclonal Antibody (Product # PA1-544) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More

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产品信息

PA1-544

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-2 µg/mL

免疫组化 (IHC)

Assay-dependent

免疫细胞化学 (ICC/IF)

2 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: C(578) E R D S P S T S R Q S P A N(592)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2243867

产品详细信息

PA1-544 detects calmodulin sensitive kinase (CASK) from mouse and rat tissues and cells.

PA1-544 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects a 112 kDa protein representing CASK from AtT20 cell lysate. Immunohistochemical staining of CASK in mouse brain with PA1-544 results in staining of the choroid plexus.

PA1-544 immunizing peptide corresponds to amino acid residues 578-592 from human CASK. This sequence is completely conserved between rat, mouse, and human CASK.

PA1-544 can be used with blocking peptide PEP-110.

靶标信息

CASK is a calcium/calmodulin-dependent serine protein kinase which is a MAGUK (membrane-associated guanylate kinase) protein family member. CASK is a scaffold protein and the encoded protein is located at synapses in the brain. CASK associates with FG syndrome 4, mental retardation, microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked mental retardation. CASK functions as a cytoskeletal membrane scaffold that coordinates signal transduction pathways within the cortical cytoskeleton.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: calcium/calmodulin-dependent serin protein kinase; Calcium/calmodulin-dependent serine protein kinase; calcium/calmodulin-dependent serine protein kinase (MAGUK family); calcium/calmodulin-dependent serine protein kinase membrane-associated guanylate kinase; hCASK; MGC7449; OTTHUMP00000023157; OTTHUMP00000023161; OTTHUMP00000023162; Peripheral plasma membrane protein CASK; Protein lin-2 homolog; RP23-278L4.1; trinucleotide repeat containing 8

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基因别名: CAGH39; CAMGUK; CASK; CMG; DXPri1; DXRib1; FGS4; LIN-2; LIN2; MICPCH; mLin-2; MRXSNA; Pals3; TNRC8

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UniProt ID: (Human) O14936, (Rat) Q62915, (Mouse) O70589

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Entrez Gene ID: (Human) 8573, (Rat) 29647, (Mouse) 12361

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