Invitrogen

CBF beta Polyclonal Antibody

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CBF beta Antibody (PA1-317) in WB

Western blot detection of CBF beta from HeLa whole cell lysates using Product # PA1-317.

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产品信息

PA1-317

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues M(1) P R V V P D Q R S K F E N E E F F R K(20) of human CBF beta.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2070590

产品详细信息

PA1-317 detects CBF-beta in human samples.

PA1-317 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~22 kDa protein representing CBF-beta in HeLa whole cell lysates.

The PA1-317 immunogen is a synthetic peptide corresponding to residues M(1) P R V V P D Q R S K F E N E E F F R K(20) of human CBF beta. This sequence is conserved in mouse. PA1-317 immunizing peptide (Cat. # PEP-282) is available for use in neutralization and control experiments.

靶标信息

The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Cancer-Associated Exosomal CBFB Facilitates the Aggressive Phenotype, Evasion of Oxidative Stress, and Preferential Predisposition to Bone Prometastatic Factor of Breast Cancer Progression.

Disease markers

Hsu CH,Ma HP,Ong JR,Hsieh MS,Yadav VK,Yeh CT,Chao TY,Lee WH,Huang WC,Kuo KT,Fong IH,Lin CC,Su CM

PA1-317 was used in Western Blotting to reveale if core binding factor subunit β may promote bone metastasis in patients with breast cancer.

Mon Aug 01 00:00:00 UTC 2022

Inhibition of Vif-Mediated Degradation of APOBEC3G through Competitive Binding of Core-Binding Factor Beta.

Journal of virology

Miyagi E,Welbourn S,Sukegawa S,Fabryova H,Kao S,Strebel K

PA1-317 was used in Western Blotting to identified CBFβ as the key player involved in D/N interference by Vif.

Tue Mar 17 00:00:00 UTC 2020

CBFβ enhances de novo protein biosynthesis of its binding partners HIV-1 Vif and RUNX1 and potentiates the Vif-induced degradation of APOBEC3G.

Journal of virology

Miyagi E,Kao S,Yedavalli V,Strebel K

PA1-317 was used in western blot to study the mechanisms underlying the effects of CBF-beta on the synthesis of HIV1 Vif and cellular RUNX and on the degradation of APOBEC3G mediated by Vif

Thu May 01 00:00:00 UTC 2014

A stable transcription factor complex nucleated by oligomeric AML1-ETO controls leukaemogenesis.

Nature

Sun XJ,Wang Z,Wang L,Jiang Y,Kost N,Soong TD,Chen WY,Tang Z,Nakadai T,Elemento O,Fischle W,Melnick A,Patel DJ,Nimer SD,Roeder RG

PA1-317 was used in western blot to study the role of AML1-ETO complex formation in the mechanism by which this transcription factor promotes leukemogenesis

Thu Aug 01 00:00:00 UTC 2013

Novel function of the unique N-terminal region of RUNX1c in B cell growth regulation.

Nucleic acids research

Brady G,Elgueta Karstegl C,Farrell PJ

PA1-317 was used in western blot to study the role of the N-terminal sequence of RUNX1c in regulating B-cell growth

Fri Feb 01 00:00:00 UTC 2013

生物信息学

蛋白别名: CBF-beta; Core-binding factor subunit beta; PEA2-beta; PEBP2-beta; polyomavirus enhancer binding protein 2, beta subunit; Polyomavirus enhancer-binding protein 2 beta subunit; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit

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基因别名: CBFB; PEBP2B

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UniProt ID: (Human) Q13951

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Entrez Gene ID: (Human) 865

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功能

DNA binding transcription factor activity, sequence-specific DNA binding transcription cofactor activity transcription coactivator activity protein binding

参与通路

protein polyubiquitination osteoblast differentiation regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter lymphocyte differentiation myeloid cell differentiation positive regulation of transcription from RNA polymerase II promoter cell maturation definitive hemopoiesis