Invitrogen

CBX2 Polyclonal Antibody

CBX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CBX2 Antibody (PA5-30996) in IHC (P)

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using CBX2 (Product # PA5-30996) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

CBX2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CBX2 Antibody — Fig. 2 Inhibition of CBX2 impairs HGSOC cell proliferation. a Model describing basic protocol for establishing adherent, suspension, and spheroid growth environments. For adherent and suspension, two verified high grade serous ovarian carcinoma cell lines were initially grown on tissue culture plastic, then distributed to normal tissue culture dishes (adherent) and polyHEMA coated culture dish (suspension) growth environments. Distributed at 1:3 or 1:5 ratio to account for forced suspension induced cell death. Photographs show PEO1 cells after 7 days in suspension (left) and HGSOC cells directly derived from patient ascites. For spheroid formation, cells were grown in 3D in Matrigel for 12 days. A representative image of a resulting spheroid is shown at upper right. b Immunoblots against CBX2 protein from OVCAR4, and PEO1 cells grown in adherent and suspension settings (described in (a)) over 7 days. c RT-qPCR for CBX2 in OVCAR4 cells transduced with small hairpin RNA (shRNA) specific for CBX2. Representative mRNA expression of CBX2 in shControl, shCBX2#1, and shCBX2#2. Statistical test = ANOVA. d Immunoblots against CBX2 protein derived from OVCAR4 shControl, shCBX2#1, and shCBX2#2 transduced cells (beta-actin = loading control). e Proliferation assay of OVCAR4 cells with CBX2 knockdown and shControl, grown in adherent setting (tissue culture plastic) over 96 h, evaluated using gLuc activity. Statistical test = ANOVA. f Same as (e), crystal violet staining and subsequent mea {KD}

产品信息

PA5-30996

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 1 and 161 of Human CBX2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.9 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2548470

产品详细信息

Recommended positive controls: A431, Jurkat.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

CBX2 identified as driver of anoikis escape and dissemination in high grade serous ovarian cancer.

Oncogenesis

Wheeler LJ,Watson ZL,Qamar L,Yamamoto TM,Post MD,Berning AA,Spillman MA,Behbakht K,Bitler BG

Published figure using CBX2 polyclonal antibody (Product # PA5-30996) in Western Blot

Mon Nov 26 00:00:00 UTC 2018

Identification of the epigenetic reader CBX2 as a potential drug target in advanced prostate cancer.

Clinical epigenetics

Clermont PL,Crea F,Chiang YT,Lin D,Zhang A,Wang JZ,Parolia A,Wu R,Xue H,Wang Y,Ding J,Thu KL,Lam WL,Shah SP,Collins CC,Wang Y,Helgason CD

PA5-30996 was used in western blot to determine a potential drug target in advanced prostate cancer by identifying the epigenetic reader CBX2

Fri Apr 22 00:00:00 UTC 2016

Identification of the epigenetic reader CBX2 as a potential drug target in advanced prostate cancer.

Clinical epigenetics

Clermont PL,Crea F,Chiang YT,Lin D,Zhang A,Wang JZ,Parolia A,Wu R,Xue H,Wang Y,Ding J,Thu KL,Lam WL,Shah SP,Collins CC,Wang Y,Helgason CD

PA5-30996 was used in western blot to determine a potential drug target in advanced prostate cancer by identifying the epigenetic reader CBX2

Fri Apr 22 00:00:00 UTC 2016

生物信息学

蛋白别名: cell division cycle associated 6; chromobox homolog 2; chromobox homolog 2 (Pc class homolog, Drosophila); Chromobox protein homolog 2; hromobox homolog 2; M33; M33 polycomb-like protein; modifier 3; Modifier 3 protein; Pc class homolog; polycomb

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基因别名: CBX2; CDCA6; M33; MOD2; pc; SRXY5

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UniProt ID: (Human) Q14781, (Mouse) P30658

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Entrez Gene ID: (Human) 84733, (Mouse) 12416

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功能

DNA binding chromatin binding protein binding methylated histone binding

参与通路

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated chromatin modification development of primary sexual characteristics covalent chromatin modification protein sumoylation cell differentiation