Invitrogen

CBX7 Recombinant Rabbit Monoclonal Antibody (JM44-63)

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CBX7 Antibody (MA5-32767) in WB

Western blot was performed using Anti-CBX7 Recombinant Rabbit Monoclonal Antibody (JM44-63) (Product # MA5-32767) and a band at 30 kDa corresponding to CBX7 was observed across the cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of A-431 (Lane 1), Hep G2 (Lane 2), HeLa (Lane 3) and COLO 205 (Lane 4) were electrophoresed using Novex® NuPAGE® 12% % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB... View More

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CBX7 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with CBX7 siRNA and reduction of signal was observed in Western Blot using CBX7 Recombinant Rabbit Monoclonal Antibody (JM44-63) (Product # MA5-32767). {KD}

产品信息

MA5-32767

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JM44-63

抗原

Recombinant protein within Human cbx7 aa 1-190

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2810044

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。