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AA Sequence of recombinant protein: SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MEC (CCL28). Anti-Human MEC (CCL28)-specific antibody was purified by affinity chromatography employing an immobilized Human MEC (CCL28) matrix.
Sandwich ELISA: To detect Human MEC (CCL28) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MEC (CCL28) (500-P297Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MEC (CCL28).
Western Blot: To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMEC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor, and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: C-C motif chemokine 28; CC chemokine CCL28; CCK 1; CCK1 protein; CCL 28; chemokine (C-C motif) ligand 28 splice variant chi; MEC; Mucosae-associated epithelial chemokine; Protein CCK1; small inducible cytokine A28; small inducible cytokine subfamily A (Cys-Cys), member 28; Small-inducible cytokine A28
基因别名: CCK1; CCL28; MEC; SCYA28
UniProt ID: (Human) Q9NRJ3
Entrez Gene ID: (Human) 56477