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CCL28 Antibody (500-P297BT-25UG) in WB
Western Blot: To detect hMEC by Western Blot analysis CCL28 Polyclonal Antibody, Biotin (Product # 500-P297BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMEC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P297BT-25UG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human MEC (CCL28)
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930288
产品详细信息
AA Sequence of recombinant protein: SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MEC (CCL28). Anti-Human MEC (CCL28)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hMEC by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MEC (500-P297) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMEC.
Western Blot: To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMEC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P297BT-1MG will be provided as 2 x 500 µg
靶标信息
MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor, and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C-C motif chemokine 28; CC chemokine CCL28; CCK 1; CCK1 protein; CCL 28; chemokine (C-C motif) ligand 28 splice variant chi; MEC; Mucosae-associated epithelial chemokine; Protein CCK1; small inducible cytokine A28; small inducible cytokine subfamily A (Cys-Cys), member 28; Small-inducible cytokine A28
基因别名: CCK1; CCL28; MEC; SCYA28
UniProt ID: (Human) Q9NRJ3
Entrez Gene ID: (Human) 56477
