Invitrogen

CCL3 Polyclonal Antibody, PeproTech®

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CCL3 Antibody (500-P77-1MG) in WB

Western Blot: To detect Rat MIP-1alpha by Western Blot analysis CCL3 Polyclonal Antibody (Product # 500-P77-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat MIP-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P77-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

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中和实验 (Neu)

- -

免疫染色 (IS)

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产品规格

种属反应

Rat

已发表种属

Rat

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Rat MIP-1alpha (CCL3)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930756

产品详细信息

AA Sequence of recombinant protein: APYGADTPTA CCFSYGRQIP RKFIADYFET SSLCSQPGVI FLTKRNRQIC ADPKETWVQE YITELELNA.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat MIP-1alpha (CCL3). Anti-Rat MIP-1alpha (CCL3)-specific antibody was purified by affinity chromatography employing an immobilized Rat MIP-1alpha (CCL3) matrix.

Sandwich ELISA: To detect Rat MIP-1alpha (CCL3) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat MIP-1alpha (CCL3) (500-P77Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat MIP-1alpha (CCL3).

Western Blot: To detect Rat MIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat MIP-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.

Journal of immunology (Baltimore, Md. : 1950)

Rius C,Abu-Taha M,Hermenegildo C,Piqueras L,Cerda-Nicolas JM,Issekutz AC,Estañ L,Cortijo J,Morcillo EJ,Orallo F,Sanz MJ

Wed Sep 15 00:00:00 UTC 2010

Menopause and ovariectomy cause a low grade of systemic inflammation that may be prevented by chronic treatment with low doses of estrogen or losartan.

Journal of immunology (Baltimore, Md. : 1950)

Abu-Taha M,Rius C,Hermenegildo C,Noguera I,Cerda-Nicolas JM,Issekutz AC,Jose PJ,Cortijo J,Morcillo EJ,Sanz MJ

Wed Jul 15 00:00:00 UTC 2009

A critical role for TNFalpha in the selective attachment of mononuclear leukocytes to angiotensin-II-stimulated arterioles.

Blood

Mateo T,Naim Abu Nabah Y,Losada M,Estellés R,Company C,Bedrina B,Cerdá-Nicolás JM,Poole S,Jose PJ,Cortijo J,Morcillo EJ,Sanz MJ

Sat Sep 15 00:00:00 UTC 2007

Possible involvement of MIP-1alpha in the recruitment of osteoclast progenitors to the distal tibia in rats with adjuvant-induced arthritis.

Laboratory investigation; a journal of technical methods and pathology

Toh K,Kukita T,Wu Z,Kukita A,Sandra F,Tang QY,Nomiyama H,Iijima T

Wed Sep 01 00:00:00 UTC 2004

Hypoxic-ischemic injury induces macrophage inflammatory protein-1alpha expression in immature rat brain.

Stroke

Cowell RM,Xu H,Galasso JM,Silverstein FS

Fri Mar 01 00:00:00 UTC 2002

Hypoxic-ischemic injury induces macrophage inflammatory protein-1alpha expression in immature rat brain.

Stroke

Cowell RM,Xu H,Galasso JM,Silverstein FS

Fri Mar 01 00:00:00 UTC 2002

Synergistic enhancement of chemokine generation and lung injury by C5a or the membrane attack complex of complement.

The American journal of pathology

Czermak BJ,Lentsch AB,Bless NM,Schmal H,Friedl HP,Ward PA

Sat May 01 00:00:00 UTC 1999

生物信息学

蛋白别名: C-C motif chemokine 3; H-MIP-1-alpha; LD78-alpha; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3); Macrophage inflammatory protein 1-alpha; MIP-1-alpha; mip1 alpha; RP23-320E6.7; small inducible cytokine A3; Small-inducible cytokine A3

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基因别名: Ccl3; MIP-1a; Mip1a; Scya3

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UniProt ID: (Rat) P50229

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Entrez Gene ID: (Rat) 25542

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功能