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CCL4 Antibody (500-P213-1MG) in WB
Western Blot: To detect Murine MIP-1beta (CCL4) by Western Blot analysis CCL4 Polyclonal Antibody (Product # 500-P213-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine MIP-1beta (CCL4) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P213-1MG
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Murine MIP-1beta (CCL4)
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929844
产品详细信息
AA Sequence of recombinant protein: APMGSDPPTS CCFSYTSRQL HRSFVMDYYE TSSLCSKPAV VFLTKRGRQI CANPSEPWVT EYMSDLELN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MIP-1beta (CCL4). Anti-Murine MIP-1beta (CCL4)-specific antibody was purified by affinity chromatography employing an immobilized Murine MIP-1beta (CCL4) matrix.
Sandwich ELISA: To detect Murine MIP-1beta (CCL4) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine MIP-1beta (CCL4) (500-P213Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MIP-1beta (CCL4).
Western Blot: To detect Murine MIP-1beta (CCL4) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine MIP-1beta (CCL4) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family. Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins. CCL4 recruits and stimulates various inflammatory cells at sites of inflammation. CCL4 is produced by lymphocytes, macrophages and dendritic cells. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ, with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells. CCL4 binds with high affinity to CCR5 receptors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ACT-2; ACT2; C-C motif chemokine 4; Immune activation protein 2; Macrophage inflammatory protein 1-beta; MGC104418; MGC126025; MGC126026; MIP-1 beta; MIP-1-beta; MIP1-beta; MIP1beta; Protein H400; RP23-320E6.8; SIS-gamma; small inducible cytokine A4; Small-inducible cytokine A4
基因别名: Act-2; AT744.1; Ccl4; MIP-1B; Mip1b; Scya4
UniProt ID: (Mouse) P14097
Entrez Gene ID: (Mouse) 20303
chemotaxis
inflammatory response
immune response
response to toxic substance
leukocyte chemotaxis
positive regulation of tumor necrosis factor production
cellular response to drug
positive regulation of vascular permeability
negative regulation by host of viral transcription
positive regulation of calcium-mediated signaling
positive regulation of calcium ion transport
positive regulation of natural killer cell chemotaxis
