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CCL4 (MIP-1 beta) Antibody (17-7540-42) in Flow
Normal human peripheral blood monocytes were unstimulated (left) or stimulated with LPS in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (right). Cells were fixed and stained intracellularly with Anti-Human CD14 PE (Product # 12-0149-42) and Anti-Human CCL4 APC using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00). Cells in the monocyte gate we... View More
产品信息
17-7540-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
FL34Z3L
抗原
Recombinant Human CCL4
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2573264
产品详细信息
Description: This FL34Z3L monoclonal antibody reacts with human CCL4. CCL4, also known as MIP-1 beta (Macrophage Inflammatory Protein 1 beta), is a member of the CC- subfamily of chemokines and is most closely related to CCL3, or MIP-1 alpha. These proteins play critical roles in the recruitment of leukocytes to the site of inflammation. While both CCL3 and CCL4 are chemoattractant for monocytes, macrophages, and dendritic cells, CCL4 preferentially attracts CD4+ T cells, while CD8+ T cells are more responsive to CCL3. In addition to its chemotactic functions, CCL4 induces the release of proinflammatory cytokine, mast cells degranulation, and NK cell activation.
CCL4 signaling is mediated by the G protein-coupled receptors CCR1, CCR4, and CCR5, which are shared with CCL3 and CCL5 (RANTES). CCR5 is the primary co-receptor for HIV entry, which the virus binds through the gp120 envelope protein. All CCR5 ligands demonstrate potent inhibition of virus entry into the cell, both through steric hindrance of gp120-CCR5 interaction, and ligand-mediated receptor internalization.
Applications Reported: This FL34Z3L antibody has been reported for use in surface and intracellular staining followed by flow cytometric analysis.
Applications Tested: This FL34Z3L antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood monocytes. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family. Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins. CCL4 recruits and stimulates various inflammatory cells at sites of inflammation. CCL4 is produced by lymphocytes, macrophages and dendritic cells. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ, with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells. CCL4 binds with high affinity to CCR5 receptors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: ACT-2; C-C motif chemokine 4; chemokine (C-C motif) ligand 4; G-26 T-lymphocyte-secreted protein; HC21; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MGC104418; MGC126025; MGC126026; MIP-1-beta; MIP-1-beta(1-69); MIP1-beta; MIP1beta; PAT 744; Protein H400; RP23-320E6.8; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2
基因别名: ACT2; AT744.1; CCL4; G-26; HC21; LAG-1; LAG1; MIP-1-beta; MIP1B; MIP1B1; SCYA2; SCYA4
UniProt ID: (Human) P13236
Entrez Gene ID: (Human) 6351
monocyte chemotaxis
movement of cell or subcellular component
inflammatory response
immune response
cell adhesion
establishment or maintenance of cell polarity
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
response to virus
response to toxic substance
neutrophil chemotaxis
positive regulation of GTPase activity
negative regulation by host of viral transcription
positive regulation of inflammatory response
positive regulation of calcium-mediated signaling
positive regulation of calcium ion transport
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
positive regulation of natural killer cell chemotaxis
