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CD10 Antibody (PA5-47075) in ICC/IF
Immunofluorescence analysis of CD10 was performed using LNCaP and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD10 Goat Polyclonal Antibody (Product # PA5-47075) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Rabbit anti-Goat IgG Heavy Chain Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27012) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green)... View More
产品信息
PA5-47075
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Sf 21-derived recombinant mouse Neprilysin/CD10 Tyr52-Trp750
偶联物
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2607390
产品详细信息
In sandwich ELISAs, less than 35% cross-reactivity with recombinant human (rh) Neprilysin is observed and less than 0.2% cross-reactivity with rhNeprilysin-2 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
靶标信息
CD10 (Common Acute Lymphocytic Leukemia Antigen, CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells. CD10 is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. Further, CD10 is a 100 kDa type II transmembrane glycoprotein that exists in a single copy of greater than 45 kb. The 5' untranslated region of the CD10 gene is alternatively spliced, resulting in four separate mRNA transcripts and the coding region is not affected by alternative splicing. Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie tooth Disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Atriopeptidase; CALLA; CD10; common acute lymphoblastic leukemia antigen; Common acute lymphocytic leukemia antigen; Enkephalinase; membrane metallo endopeptidase; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); membrane metallo-endopeptidase (neutral endopeptidase/enkephalinase); membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; NEP; Neprilysin; neprilysin-390; neprilysin-411; Neutral endopeptidase 24.11; SFE; Skin fibroblast elastase
基因别名: 6030454K05Rik; C85356; CALLA; CD10; CMT2T; EPN; MME; NEP; SFE
UniProt ID: (Human) P08473, (Mouse) Q61391, (Rat) P07861
Entrez Gene ID: (Human) 4311, (Mouse) 17380, (Rat) 24590
