Invitrogen

CD100 Polyclonal Antibody

CD100 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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CD100 Antibody (PA5-47711) in IHC (F)

Immunohistochemical analysis of CD100 in immersion fixed frozen sections of mouse embryonic eye cup (15 d.p.c.). Samples were incubated in CD100 polyclonal antibody (Product # PA5-47711) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm.

CD100 Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-47711

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1 µg/mL

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

5-15 µg/mL

流式细胞分析 (Flow)

2.5 µg per million cells

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Sheep / IgG

分类

Polyclonal

类型

Antibody

抗原

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4D/CD100 Phe24-Thr657

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2610477

产品详细信息

In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Semaphorin 3A, rhSemaphorin 4B, and rhSemaphrin 6C is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

靶标信息

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Semaphorin 4D is upregulated in neurons of diseased brains and triggers astrocyte reactivity.

Journal of neuroinflammation

Evans EE,Mishra V,Mallow C,Gersz EM,Balch L,Howell A,Reilly C,Smith ES,Fisher TL,Zauderer M

PA5-47711 was used in Immunohistochemistry to suggest that SEMA4D blockade may ameliorate disease pathology by preserving normal astrocyte function and reducing the negative consequences of reactive astrogliosis.

Sat Aug 06 00:00:00 UTC 2022

Semaphorin 4D is upregulated in neurons of diseased brains and triggers astrocyte reactivity.

Journal of neuroinflammation

Evans EE,Mishra V,Mallow C,Gersz EM,Balch L,Howell A,Reilly C,Smith ES,Fisher TL,Zauderer M

Sat Aug 06 00:00:00 UTC 2022

Anti-Semaphorin 4D Rescues Motor, Cognitive, and Respiratory Phenotypes in a Rett Syndrome Mouse Model.

International journal of molecular sciences

Mao Y,Evans EE,Mishra V,Balch L,Eberhardt A,Zauderer M,Gold WA

PA5-47711 was used in Immunohistochemistry to suggest that anti-SEMA4D immunotherapy may be an effective treatment option to alleviate symptoms and improve cognitive and motor function in Rett syndrome.