Invitrogen

CD100 (SEMA4D) Monoclonal Antibody (BMA12 (BMA-12)), eBioscience™

CD100 (SEMA4D) Antibody in Flow Cytometry (Flow)

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CD100 (SEMA4D) Antibody (14-1001-82) in Flow

Staining of C57BL/6 splenocytes with 0.125 µg of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.125 µg of Anti-Mouse CD100 (SEMA4D) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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产品信息

14-1001-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

Assay-Dependent

流式细胞分析 (Flow)

0.25 µg/test

免疫沉淀 (IP)

Assay-Dependent

功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

BMA12 (BMA-12)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_1603189

产品详细信息

Description: The BMA12 monoclonal antibody reacts with mouse CD100, also known as Sema4D. Sema4D/CD100 is a 150kDa transmembrane protein expressed constitutively on T cells and is involved in the activation of B cells through its interaction with CD72. Interaction of these two proteins diminishes the negative signaling normally effected by CD72. CD100 is already expressed strongly on resting T cells and somewhat weakly on B cells, but activation upregulates expression on both cell types. Antigen expression has been reported in Spleen, thymus and lymph nodes.

Applications Reported: The BMA12 (BMA-12) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. It has also been reported for use in neutralization of mouse CD100. (Please use Functional Grade purified BMA12, Product # 16-1001, in functional assays).

Applications Tested: The BMA12 (BMA-12) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

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