Invitrogen

CD100 (SEMA4D) Monoclonal Antibody (eBio133-1C6 (133-1C6)), Alexa Fluor™ 488, eBioscience™

CD100 (SEMA4D) Antibody in Flow Cytometry (Flow)

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CD100 (SEMA4D) Antibody (53-1009-42) in Flow

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD100 Alexa Fluor® 488 (filled histogram). Cells in the lymphocyte gate were used for analysis.

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产品信息

53-1009-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

eBio133-1C6 (133-1C6)

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10853965

产品详细信息

Description: The eBio133-1C6 monoclonal antibody reacts with human CD100, a member of the class IV semaphorin family also called sema4D. CD100 promotes angiogenesis, invasive growth, proliferation, and anti-apoptosis of cancer cells in vitro. Furthermore CD100 is a costimulatory molecule in adaptive immune responses by switching off negative signalling from CD72. In addition to CD72 another ligand is Plexin-B1. It has been shown that higher expression levels of CD100 are correlated with poor survival in soft tissue sarcoma patients. Expression is found in both hematopoietic and non-hematopoietic cells.CD100 is expressed on resting T cells and NK cells and B cell. CD100 can also exist as a soluble form.

Applications Reported: This eBio133-1C6 (133-1C6) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio133-1C6 (133-1C6) antibody has been pre-titrated and tested by flow cytometric of human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Tax and Semaphorin 4D Released from Lymphocytes Infected with Human Lymphotropic Virus Type 1 and Their Effect on Neurite Growth.

AIDS research and human retroviruses

Quintremil S,Alberti C,Rivera M,Medina F,Puente J,Cartier L,Ramírez E,Tanaka Y,Valenzuela MA

53-1009 was used in Flow cytometry/Cell sorting to demonstrate that OSMRβ in neurons is critical for neuronal survival during cerebral ischemic/reperfusion.

Fri Jan 01 00:00:00 UTC 2016

生物信息学

蛋白别名: A8; BB18; CD100; GR3; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; Semaphorin-4D

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基因别名: C9orf164; CD100; coll-4; M-sema-G; SEMA4D; SEMAJ

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UniProt ID: (Human) Q92854

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Entrez Gene ID: (Human) 10507

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功能

receptor activity transmembrane signaling receptor activity receptor binding protein binding semaphorin receptor binding neuropilin binding chemorepellent activity membrane-bound signaling molecule intercellular signal molecule

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