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CD101 Antibody (MA5-55405) in WB
Western blot analysis of lysates from 293T-CD101 cells, using CD101 Recombinant Monoclonal Antibody (Product # MA5-55405) at 1:1,000 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
产品信息
MA5-55405
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
5D6V4
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 21-410 of human CD101.
偶联物
形式
Liquid
浓度
1.08 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
内含物
0.05% ProClin 300
保存条件
-20° C, Avoid Freeze/Thaw Cycles
产品详细信息
Immunogen Sequence: QREVTVQKGP LFRAEGYPVS IGCNVTGHQG PSEQHFQWSV YLPTNPTQEV QIISTKDAAF SYAVYTQRVR SGDVYVERVQ GNSVLLHISK LQMKDAGEYE CHTPNTDEKY YGSYSAKTNL IVIPDTLSAT MSSQTLGKEE GEPLALTCEA SKATAQHTHL SVTWYLTQDG GGSQATEIIS LSKDFILVPG PLYTERFAAS DVQLNKLGPT TFRLSIERLQ SSDQGQLFCE ATEWIQDPDE TWMFITKKQT DQTTLRIQPA VKDFQVNITA DSLFAEGKPL ELVCLVVSSG RDPQLQGIWF FNGTEIAHID AGGVLGLKND YKERASQGEL QVSKLGPKAF SLKIFSLGPE DEGAYRCVVA EVMKTRTGSW QVLQRKQSPD SHVHLRKPAA
靶标信息
CD101 also known as V7 antigen, a member of the Ig superfamily. CD101 is a disulfide linked homodimer of unknown function. Expression of CD101 is found on monocytes, granulocytes and dendritic cells (Langerhan-like cells HLA-DR, CD1a, CD1c). In addition expression on T lymphocytes is important for cell activation. CD101+ CD28+ cells are very responsive to CD28 signaling. In combination with anti-CD28 or suboptimal levels of anti-CD3, anti-CD101 can increase proliferation thereby suggesting an activating role. The monoclonal antibody BB27 has been shown to inhibit the T cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures. Recently it has been demonstrated that mouse CD101 is found on a subpopulation of regulatory T cells (CD4, CD25, Foxp3 positive) that have high suppressor activity. Expression of CD101 on human PBMCs shows staining of about 30% of the Foxp3 positive cells. Studies have not confirmed higher suppressor activity in the human CD101 population.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD101; Cell surface glycoprotein V7; EWI-101; Glu-Trp-Ile EWI motif-containing protein 101; IgSF2; Immunoglobulin superfamily member 2; leukocyte surface protein; RP11-27K13.2
基因别名: CD101; EWI-101; EWI101; IGSF2; V7
UniProt ID: (Human) Q93033
Entrez Gene ID: (Human) 9398
