Invitrogen

CD103 (Integrin alpha E) Monoclonal Antibody (OX62), PE, eBioscience™

CD103 (Integrin alpha E) Antibody in Flow Cytometry (Flow)

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CD103 (Integrin alpha E) Antibody (12-1030-82) in Flow

Staining of rat splenocytes with Anti-Rat CD4 FITC (Product # 11-0040-82) and 0.125 µg of Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (left) or 0.125 µg of Anti-Rat CD103 (Integrin alpha E) PE (right). Cells in the lymphocyte gate were used for analysis and events are displayed after gating out CD3+ T cells and CD45RB+ cells and gating on the remaining cells, which are MHC Class II+.

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产品信息

12-1030-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Rat

已发表种属

Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OX62

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1210757

产品详细信息

Description: The OX62 monoclonal antibody reacts with rat CD103, also known as alphaE2 integrin. CD103 was originally identified on rat veiled dendritic cells, and is expressed by dendritic epidermal T cells in the skin, intraepithelial lymphocytes in the small intestine and by dendritic cells associated with gammadelta T cells. Integrins are a family of heterodimeric proteins which contain non-covalently associated alpha and beta transmembrane glycoproteins. Integrins function as receptors for extracellular matrix and cell-cell adhesion molecules.

Applications Reported: This OX62 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX62 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD103 (ITGAE, Integrin alpha E) is molecule that is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. In particular, CD103 includes a 150kDa alpha chain and a 120kDa beta chain. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is part of the integrin family which are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103 protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Resveratrol regulates macrophage recruitment and M1 macrophage polarization and prevents corneal allograft rejection in rats.

Frontiers in medicine

Xu C,Guo R,Hou C,Ma M,Dong X,Ouyang C,Wu J,Huang T

12-1030-82 was used in Flow Cytometry to suggest that resveratrol can reduce corneal macrophage recruitment and M1 macrophage polarization after corneal transplantation in rats and prevent CGR.

Thu Nov 09 00:00:00 UTC 2023

Cell release during perfusion reflects cold ischemic injury in rat livers.

Scientific reports

de Vries RJ,Pendexter CA,Cronin SEJ,Marques B,Hafiz EOA,Muzikansky A,van Gulik TM,Markmann JF,Stott SL,Yeh H,Toner M,Uygun K,Tessier SN

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 12-1030-82) in Flow Cytometry

Fri Jan 24 00:00:00 UTC 2020

Role of CCR7 on dendritic cell‑mediated immune tolerance in the airways of allergy‑induced asthmatic rats.

Molecular medicine reports

Li Y,Du Y,Zhang A,Jiang R,Nie X,Xiong X

12-1030-82 was used in Flow cytometry to investigate the effects of CC chemokine receptor 7 on Dendritic cells‚mediated immune tolerance in allergic asthma.

Fri Nov 01 00:00:00 UTC 2019

Amelioration of collagen-induced arthritis using antigen-loaded dendritic cells modified with NF-κB decoy oligodeoxynucleotides.

Drug design, development and therapy

Jiang H,Hu H,Zhang Y,Yue P,Ning L,Zhou Y,Shi P,Yuan R

12-1030 was used in Flow cytometry/Cell sorting to investigate the therapeutic effect of dendritic cells loaded with bovine type II collagen on collagen-induced arthritis in rats.

Mon Jul 23 00:00:00 UTC 2018

Immunologically augmented skin flap as a novel dendritic cell vaccine against head and neck cancer in a rat model.

Cancer science

Inoue K,Saegusa N,Omiya M,Ashizawa T,Miyata H,Komiyama M,Iizuka A,Kume A,Sugino T,Yamaguchi K,Kiyohara Y,Nakagawa M,Akiyama Y

12-1030 was used in Flow cytometry/Cell sorting to suggest that a novel immunotherapy based on the immuno-flap method has the potential for clinical application to prevent the local recurrence of head and neck cancer patients.

Sun Feb 01 00:00:00 UTC 2015

Interleukin 23 production by intestinal CD103(+)CD11b(+) dendritic cells in response to bacterial flagellin enhances mucosal innate immune defense.

Immunity

Kinnebrew MA,Buffie CG,Diehl GE,Zenewicz LA,Leiner I,Hohl TM,Flavell RA,Littman DR,Pamer EG

12-1030 was used in Flow cytometry/Cell sorting to demonstrate cytokine expression by certain DCs drives IL-22-dependent RegIIIγ production for protection from bacterial colonisation.

Fri Feb 24 00:00:00 UTC 2012

生物信息学

蛋白别名: DC; DCs; integrin alpha E1, epithelial-associated; integrin alpha-E; integrin, alpha E, epithelial-associated; Intregrin alpha E2; MGC141996

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Entrez Gene ID: (Rat) 83577

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功能

molecular_function integrin

参与通路

cell adhesion integrin-mediated signaling pathway