Invitrogen

CD106 (VCAM-1) Monoclonal Antibody (429), Functional Grade, eBioscience™

CD106 (VCAM-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

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CD106 (VCAM-1) Antibody (16-1061-82) in ICC/IF, Flow

Nature communications 2017 - Figure 3 Loss of endothelial SENP1 inhibits EC activation. ( a ) Grafts from WT or SENP1-ecKO mice were harvested 3 days post-transplantation. The induction of endothelial adhesion molecules was demonstrated by immunofluorescence staining of ICAM-1, VCAM-1, or P-selectin and PECAM-1 with DAPI labelling of the nuclei. Bar represents 50 mum. ( b - e ) Attenuated induction of adhesion molecules in SENP1-ecKO M... View More

CD106 (VCAM-1) Antibody in Immunohistochemistry (IHC)

CD106 (VCAM-1) Antibody in Flow Cytometry (Flow)

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产品信息

16-1061-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看9篇已发表文章 9篇已发表文章

中和实验 (Neu)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

功能检测 (Functional)

Assay-Dependent

抑制试剂 (IA)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

429

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_469044

产品详细信息

Description: The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin a4b7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported: The 429 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of CD106 in functional studies.

Applications Tested: The 429 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

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Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 16-1061-82) in Flow Cytometry

Sun Apr 17 00:00:00 UTC 2022

Inhibition of the JNK/MAPK signaling pathway by myogenesis-associated miRNAs is required for skeletal muscle development.

Cell death and differentiation

Xie SJ,Li JH,Chen HF,Tan YY,Liu SR,Zhang Y,Xu H,Yang JH,Liu S,Zheng LL,Huang MB,Guo YH,Zhang Q,Zhou H,Qu LH

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 16-1061-82) in Flow Cytometry

Sat Sep 01 00:00:00 UTC 2018

Low-Dose Radiotherapy Has No Harmful Effects on Key Cells of Healthy Non-Inflamed Joints.

International journal of molecular sciences

Deloch L,Rückert M,Fietkau R,Frey B,Gaipl US

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 16-1061-82) in Flow Cytometry

Tue Oct 16 00:00:00 UTC 2018

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 16-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 16-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 16-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 16-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

16-1061 was used in Blocking experiments to studying Treg cell engagement with the lymphotoxin beta receptor in transendothelial migration.

Tue Jun 21 00:00:00 UTC 2016

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

16-1061 was used in Blocking experiments to studying Treg cell engagement with the lymphotoxin beta receptor in transendothelial migration.

Tue Jun 21 00:00:00 UTC 2016

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

16-1061 was used in Blocking experiments to studying Treg cell engagement with the lymphotoxin beta receptor in transendothelial migration.

Tue Jun 21 00:00:00 UTC 2016

Distinct in vitro properties of embryonic and extraembryonic fibroblast-like cells are reflected in their in vivo behavior following grafting in the adult mouse brain.

Cell transplantation

Costa R,Bergwerf I,Santermans E,De Vocht N,Praet J,Daans J,Le Blon D,Hoornaert C,Reekmans K,Hens N,Goossens H,Berneman Z,Parolini O,Alviano F,Ponsaerts P

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 16-1061-82) in Flow Cytometry

Mon Nov 16 00:00:00 UTC 2015

Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.

Cell

Haldar M,Kohyama M,So AY,Kc W,Wu X,Briseño CG,Satpathy AT,Kretzer NM,Arase H,Rajasekaran NS,Wang L,Egawa T,Igarashi K,Baltimore D,Murphy TL,Murphy KM

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 16-1061-82) in Flow Cytometry

Thu Mar 13 00:00:00 UTC 2014

Effects of intra-abdominal sepsis on atherosclerosis in mice.

Critical care (London, England)

Kaynar AM,Yende S,Zhu L,Frederick DR,Chambers R,Burton CL,Carter M,Stolz DB,Agostini B,Gregory AD,Nagarajan S,Shapiro SD,Angus DC

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 16-1061-82) in Immunofluorescence

Wed Sep 03 00:00:00 UTC 2014

Nrf2 regulates haematopoietic stem cell function.

Nature cell biology

Tsai JJ,Dudakov JA,Takahashi K,Shieh JH,Velardi E,Holland AM,Singer NV,West ML,Smith OM,Young LF,Shono Y,Ghosh A,Hanash AM,Tran HT,Moore MA,van den Brink MR

16-1061 was used in Flow cytometry/Cell sorting to investigate the link between Nrf2 and CXCR4, thought to partially contribute to the maintenance of haematopoietic stem cell function.

Fri Mar 01 00:00:00 UTC 2013

Rhodamine-loaded intercellular adhesion molecule-1-targeted microbubbles for dual-modality imaging under controlled shear stresses.

Circulation. Cardiovascular imaging

Wu Z,Curaj A,Fokong S,Liehn EA,Weber C,Lammers T,Kiessling F,Zandvoort van M

16-1061 was used in Immunohistochemistry on frozen tissues to demonstrate the benefits of molecular ultrasound imaging for visualising blood vessels with high shear stress and flow rates.

Fri Nov 01 00:00:00 UTC 2013

Rhodamine-loaded intercellular adhesion molecule-1-targeted microbubbles for dual-modality imaging under controlled shear stresses.

Circulation. Cardiovascular imaging

Wu Z,Curaj A,Fokong S,Liehn EA,Weber C,Lammers T,Kiessling F,Zandvoort van M

16-1061 was used in Immunohistochemistry on frozen tissues to demonstrate the benefits of molecular ultrasound imaging for visualising blood vessels with high shear stress and flow rates.

Fri Nov 01 00:00:00 UTC 2013

Rhodamine-loaded intercellular adhesion molecule-1-targeted microbubbles for dual-modality imaging under controlled shear stresses.

Circulation. Cardiovascular imaging

Wu Z,Curaj A,Fokong S,Liehn EA,Weber C,Lammers T,Kiessling F,Zandvoort van M

16-1061 was used in Immunohistochemistry on frozen tissues to demonstrate the benefits of molecular ultrasound imaging for visualising blood vessels with high shear stress and flow rates.