Invitrogen

CD106 (VCAM-1) Monoclonal Antibody (429), eFluor™ 660, eBioscience™

CD106 (VCAM-1) Antibody in Flow Cytometry (Flow)

图: 1 / 9

CD106 (VCAM-1) Antibody (50-1061-82) in Flow

Staining of C57Bl/6 bone marrow cells with 0.25 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (blue histogram) or 0.25 µg of Anti-Mouse CD106 (VCAM-1) eFluor® 660 (purple histogram). Total viable cells were used for analysis.

CD106 (VCAM-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD106 (VCAM-1) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

50-1061-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看9篇已发表文章 9篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

429

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11219864

产品详细信息

Description: The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4 beta1 (VLA-4, CD49d/CD29) and Integrin a4b7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported: This 429 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 429 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Extracellular HMGB-1 activates inflammatory signaling in tendon cells and tissues.

Therapeutic advances in chronic disease

Zhang C,Gu X,Zhao G,Wang W,Shao J,Zhu J,Yuan T,Sun J,Nie D,Zhou Y

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 50-1061-82) in Flow Cytometry

Sun Apr 17 00:00:00 UTC 2022

Inhibition of the JNK/MAPK signaling pathway by myogenesis-associated miRNAs is required for skeletal muscle development.

Cell death and differentiation

Xie SJ,Li JH,Chen HF,Tan YY,Liu SR,Zhang Y,Xu H,Yang JH,Liu S,Zheng LL,Huang MB,Guo YH,Zhang Q,Zhou H,Qu LH

50-1061 was used in Flow cytometry/Cell sorting to reveal a double-negative feedback regulatory pattern of cell-specific miRNAs by targeting phosphorylation kinase signaling cascade responsible for skeletal muscle development.

Sat Sep 01 00:00:00 UTC 2018

Low-Dose Radiotherapy Has No Harmful Effects on Key Cells of Healthy Non-Inflamed Joints.

International journal of molecular sciences

Deloch L,Rückert M,Fietkau R,Frey B,Gaipl US

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 50-1061-82) in Flow Cytometry

Tue Oct 16 00:00:00 UTC 2018

Mesenchymal stromal cells-derived matrix Gla protein contribute to the alleviation of experimental colitis.

Cell death & disease

Feng Y,Liao Y,Huang W,Lai X,Luo J,Du C,Lin J,Zhang Z,Qiu D,Liu Q,Shen H,Xiang AP,Zhang Q

50-1061 was used in Flow cytometry/Cell sorting to study the role of matrix Gla protein as a novel mediator of mesenchymal stromal cell-mediated immunomodulation in treating Crohn's disease.

Thu Jun 07 00:00:00 UTC 2018

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 50-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 50-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 50-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 50-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis.

Cell adhesion & migration

Hintermann E,Bayer M,Ehser J,Aurrand-Lions M,Pfeilschifter JM,Imhof BA,Christen U

50-1061 was used in Flow cytometry/Cell sorting to show that during liver fibrogenesis, JAM-B and JAM-C expression increases on the vascular endothelium.

Sun Jul 03 00:00:00 UTC 2016

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 50-1061-82) in Flow Cytometry

Tue Jun 21 00:00:00 UTC 2016

Distinct in vitro properties of embryonic and extraembryonic fibroblast-like cells are reflected in their in vivo behavior following grafting in the adult mouse brain.

Cell transplantation

Costa R,Bergwerf I,Santermans E,De Vocht N,Praet J,Daans J,Le Blon D,Hoornaert C,Reekmans K,Hens N,Goossens H,Berneman Z,Parolini O,Alviano F,Ponsaerts P