Invitrogen

CD106 (VCAM-1) Monoclonal Antibody (eBioMR106 (MR106)), Biotin, eBioscience™

CD106 (VCAM-1) Antibody in Flow Cytometry (Flow)

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CD106 (VCAM-1) Antibody (13-1060-82) in Flow

Staining of rat bone marrow cells with 0.125 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (left) or 0.125 µg of Anti-Rat CD106 (VCAM-1) Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD106 (VCAM-1) Antibody in Immunocytochemistry (ICC/IF)

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产品信息

13-1060-82

应用

建议稀释比

已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.25 µg/test

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功能检测 (Functional)

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产品规格

种属反应

Rat

已发表种属

Human, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioMR106 (MR106)

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_529468

产品详细信息

Description: The eBioMR106 monoclonal antibody reacts with rat CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and further upregulate VCAM-1 upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported: This eBioMR106 (MR106) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioMR106 (MR106) antibody has been tested by flow cytometric analysis of rat bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Blood-brain barrier dysfunction in L-ornithine induced acute pancreatitis in rats and the direct effect of L-ornithine on cultured brain endothelial cells.

Fluids and barriers of the CNS

Walter FR,Harazin A,Tóth AE,Veszelka S,Santa-Maria AR,Barna L,Kincses A,Biczó G,Balla Z,Kui B,Maléth J,Cervenak L,Tubak V,Kittel Á,Rakonczay Z,Deli MA

13-1060-82 was used in Immunohistochemistry (PFA fixed) to suggest a direct effect of the cationic L-ornithine on brain endothelium. Endothelial surface glycocalyx injury was revealed both in vivo and in vitro, as an additional novel component of the BBB-related pathological changes in AP.

Thu Feb 17 00:00:00 UTC 2022

Blood-brain barrier dysfunction in L-ornithine induced acute pancreatitis in rats and the direct effect of L-ornithine on cultured brain endothelial cells.

Fluids and barriers of the CNS

Walter FR,Harazin A,Tóth AE,Veszelka S,Santa-Maria AR,Barna L,Kincses A,Biczó G,Balla Z,Kui B,Maléth J,Cervenak L,Tubak V,Kittel Á,Rakonczay Z,Deli MA

Thu Feb 17 00:00:00 UTC 2022

Early Proteome Shift and Serum Bioactivity Precede Diesel Exhaust-induced Impairment of Cardiovascular Recovery in Spontaneously Hypertensive Rats.

Scientific reports

Thompson LC,Shannahan JH,Perez CM,Haykal-Coates N,King C,Hazari MS,Brown JM,Farraj AK

13-1060-82 was used in Flow Cytometry to determine if exposure-induced cardiovascular dysfunction can be coupled with alterations in both serum bioactivity and the circulating proteome.

Fri May 03 00:00:00 UTC 2019

Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms.

Stem cells international

Labedz-Maslowska A,Kamycka E,Bobis-Wozowicz S,Madeja Z,Zuba-Surma EK

13-1060 was used in Flow cytometry/Cell sorting to identify multiantigenic phenotype and providing sorting protocols for isolation VSELs from rat BM tissue for further examining of their functional properties.

Fri Dec 04 00:00:00 UTC 2015

From the Cover: Disease-Induced Disparities in Formation of the Nanoparticle-Biocorona and the Toxicological Consequences.

Toxicological sciences : an official journal of the Society of Toxicology

Shannahan JH,Fritz KS,Raghavendra AJ,Podila R,Persaud I,Brown JM

Mon Aug 01 00:00:00 UTC 2016

From the Cover: Disease-Induced Disparities in Formation of the Nanoparticle-Biocorona and the Toxicological Consequences.

Toxicological sciences : an official journal of the Society of Toxicology

Shannahan JH,Fritz KS,Raghavendra AJ,Podila R,Persaud I,Brown JM

Mon Aug 01 00:00:00 UTC 2016

Temporal pattern of ICAM-I mediated regulatory T cell recruitment to sites of inflammation in adoptive transfer model of multiple sclerosis.

PloS one

Doerck S,Göbel K,Weise G,Schneider-Hohendorf T,Reinhardt M,Hauff P,Schwab N,Linker R,Mäurer M,Meuth SG,Wiendl H

13-1060 was used in Functional assays to explore the migration of pro-inflammatory and regulatory T cells towards target organs in an in vivo model of multiple sclerosis.