Invitrogen

CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE-Cyanine7, eBioscience™

CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)

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CD107a (LAMP-1) Antibody (25-1079-42) in Flow

Staining of Jurkat cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD107a (LAMP-1) PE-Cyanine7 (purple histogram). Total cells were used for analysis.

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产品信息

25-1079-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioH4A3

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2573384

产品详细信息

Description: The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated protein of approximately 110kDa. It is predominantly expressed intracellularly in the lysosomal/endosomal membrane in nearly all cells. CD107a is transiently expressed on the cell surface of degranulating cytolytic T cells, and is also upregulated on the surface of activated platelets and some cancer cells.

Applications Reported: This eBioH4A3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBioH4A3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer’s Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Soluble NKG2D ligands impair CD8⁺ T cell antitumor function dependent of NKG2D downregulation in neuroblastoma.

Oncology letters

Zhang Y,Luo F,Dong K

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 25-1079-42) in Flow Cytometry

Sat Jul 01 00:00:00 UTC 2023

Is IFN expression by NK cells a hallmark of severe COVID-19?

Cytokine

Csordas BG,de Sousa Palmeira PH,Peixoto RF,Comberlang FCQDDS,de Medeiros IA,Azevedo FLAA,Veras RC,Janebro DI,Amaral IPG,Barbosa-Filho JM,Keesen TSL

25-1079-42 was used in Flow cytometry/Cell sorting to demonstrate that CD3-CD56+ NK cells frequency in the volunteers who recovered from mild COVID-19 (Mild CoV) presented a significant increase compared to the healthy control (HC) and individuals recovering from severe COVID-19 (Severe CoV) groups.

Thu Sep 01 00:00:00 UTC 2022

Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab.

Signal transduction and targeted therapy

Zheng G,Guo Z,Li W,Xi W,Zuo B,Zhang R,Wen W,Yang AG,Jia L

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 25-1079-42) in Flow Cytometry

Wed Jun 23 00:00:00 UTC 2021

Circulating prostate cancer cells have differential resistance to fluid shear stress-induced cell death.

Journal of cell science

Hope JM,Bersi MR,Dombroski JA,Clinch AB,Pereles RS,Merryman WD,King MR

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 25-1079-42) in Flow Cytometry

Mon Feb 22 00:00:00 UTC 2021

HLA-B*27:05 alters immunodominance hierarchy of universal influenza-specific CD8+ T cells.

PLoS pathogens

Sant S,Quiñones-Parra SM,Koutsakos M,Grant EJ,Loudovaris T,Mannering SI,Crowe J,van de Sandt CE,Rimmelzwaan GF,Rossjohn J,Gras S,Loh L,Nguyen THO,Kedzierska K

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 25-1079-42) in Flow Cytometry

Sat Aug 01 00:00:00 UTC 2020

PBRM1 and the glycosylphosphatidylinositol biosynthetic pathway promote tumor killing mediated by MHC-unrestricted cytotoxic lymphocytes.

Science advances

Menasche BL,Davis EM,Wang S,Ouyang Y,Li S,Yu H,Shen J

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 25-1079-42) in Flow Cytometry

Sun Nov 01 00:00:00 UTC 2020

MAVS Genetic Variation Is Associated with Decreased HIV-1 Replication In Vitro and Reduced CD4⁺ T Cell Infection in HIV-1-Infected Individuals.

Viruses

Stunnenberg M,van Pul L,Sprokholt JK,van Dort KA,Gringhuis SI,Geijtenbeek TBH,Kootstra NA

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 25-1079-42) in Flow Cytometry

Thu Jul 16 00:00:00 UTC 2020

PD1 is expressed on exhausted T cells as well as virus specific memory CD8+ T cells in the bone marrow of myeloma patients.

Oncotarget

Sponaas AM,Yang R,Rustad EH,Standal T,Thoresen AS,Dao Vo C,Waage A,Slørdahl TS,Børset M,Sundan A

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 25-1079-42) in Flow Cytometry

Fri Aug 10 00:00:00 UTC 2018

Complement receptor CD46 co-stimulates optimal human CD8⁺ T cell effector function via fatty acid metabolism.

Nature communications

Arbore G,West EE,Rahman J,Le Friec G,Niyonzima N,Pirooznia M,Tunc I,Pavlidis P,Powell N,Li Y,Liu P,Servais A,Couzi L,Fremeaux-Bacchi V,Placais L,Ferraro A,Walsh PR,Kavanagh D,Afzali B,Lavender P,Lachmann HJ,Kemper C

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 25-1079-42) in Flow Cytometry

Wed Oct 10 00:00:00 UTC 2018

T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma.

Oncoimmunology

Singh N,Kulikovskaya I,Barrett DM,Binder-Scholl G,Jakobsen B,Martinez D,Pawel B,June CH,Kalos MD,Grupp SA

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 25-1079-42) in Flow Cytometry

Tue Jan 12 00:00:00 UTC 2021

Inhibition of CD39 enzymatic function at the surface of tumor cells alleviates their immunosuppressive activity.

Cancer immunology research

Bastid J,Regairaz A,Bonnefoy N,Déjou C,Giustiniani J,Laheurte C,Cochaud S,Laprevotte E,Funck-Brentano E,Hemon P,Gros L,Bec N,Larroque C,Alberici G,Bensussan A,Eliaou JF

25-1079 was used in Flow cytometry/Cell sorting to address whether CD39 is expressed by tumour cells and whether CD39+ tumour cells mediate immunosuppression via the adenosine pathway.

Sun Mar 01 00:00:00 UTC 2015

生物信息学

蛋白别名: CD107 antigen-like family member A; CD107a; LAMP-1; lysosomal-associated membrane protein 1; Lysosome-associated membrane glycoprotein 1; lysosome-associated membrane protein 1

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基因别名: CD107a; LAMP1; LAMPA; LGP120

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UniProt ID: (Human) P11279

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Entrez Gene ID: (Human) 3916

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功能

virus receptor activity protein binding enzyme binding protein domain specific binding membrane trafficking regulatory protein

参与通路