Invitrogen

CD11b Monoclonal Antibody (M1/70.15), Biotin

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CD11b Antibody (MA5-17858) in Flow

Flow cytometric analysis of C3H/HE mouse peritoneal macrophages (left) and bone marrow (right) were stained using a CD11b/ITGAM/Integrin alpha M monoclonal antibody (Product # MA5-17858) (filled histogram) or rat IgG2b isotype control (open histogram).

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产品信息

MA5-17858

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫组化（冰冻） (IHC (F))

Assay-dependent

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流式细胞分析 (Flow)

0.2 µg/1x10^6 cells

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亲和纯化 (AP)

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其他PubMed (Misc)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

M1/70.15

抗原

C57BL/10 spleen cell enriched for T lymphocytes

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偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Protein G

保存液

PBS with 4-5mg/mL BSA

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2539242

产品详细信息

MA5-17858 targets CD11b/ITGAM/Integrin alpha M in FACS, IHC (F), and IHC (P) applications and shows reactivity with Mouse samples.

The MA5-17858 immunogen is c57BL/10 spleen cell enriched for T lymphocytes.

靶标信息

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Excessive immunosuppression by regulatory T cells antagonizes T cell response to schistosome infection in PD-1-deficient mice.

PLoS pathogens

Lu L,Li T,Feng X,Liu Z,Liu Y,Chao T,Gu Y,Huang R,Zhang F,He L,Zhou B,Kong E,Liu Z,Wang X,Chen Z,Wang H,Malissen M,Malissen B,Zhang L,Liang Y

MA5-17858 was used in Affinity Purification to provide novel insights into prevention and treatment of schistosomiasis on the basis of modulating host adaptive immunity.

Wed Jun 01 00:00:00 UTC 2022

The presence of IL-17A and T helper 17 cells in experimental mouse brain tumors and human glioma.

PloS one

Wainwright DA,Sengupta S,Han Y,Ulasov IV,Lesniak MS

MA5-17858 was used in immunohistochemistry - frozen section to study Th17 cells in glia-derived tumors of the central nervous system

Sat Oct 23 00:00:00 UTC 2010

Chemokine receptor CXCR5 supports solitary intestinal lymphoid tissue formation, B cell homing, and induction of intestinal IgA responses.

Journal of immunology (Baltimore, Md. : 1950)

Velaga S,Herbrand H,Friedrichsen M,Jiong T,Dorsch M,Hoffmann MW,Förster R,Pabst O

MA5-17858 was used in flow cytometry to characterize and track solitary intestinal lymphoid tissues

Sun Mar 01 00:00:00 UTC 2009

Lack of antigen-specific tissue remodeling in mice deficient in the macrophage galactose-type calcium-type lectin 1/CD301a.

Blood

Sato K,Imai Y,Higashi N,Kumamoto Y,Onami TM,Hedrick SM,Irimura T

MA5-17858 was used in flow cytometry to investigate the contribution of macrophage galactose-type C-type lectin1 to granulation tissue formation

Fri Jul 01 00:00:00 UTC 2005

Granulation tissue formation by nonspecific inflammatory agent occurs independently of macrophage galactose-type C-type lectin-1.

Clinical immunology (Orlando, Fla.)

Sato K,Komatsu N,Higashi N,Imai Y,Irimura T

MA5-17858 was used in immunohistochemistry - frozen section to determine the role of a macrophage galactose-type calcium-type lectin-1 in antigen-independent granulation tissue formation

Fri Apr 01 00:00:00 UTC 2005

Host conditioning with total lymphoid irradiation and antithymocyte globulin prevents graft-versus-host disease: the role of CD1-reactive natural killer T cells.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Lan F,Zeng D,Higuchi M,Higgins JP,Strober S

MA5-17858 was used in flow cytometry to test if a total lymphoid irradiation and anti-thymocyte serum regimen can prevent graft verses host disease.

Sun Jun 01 00:00:00 UTC 2003

Transgenic expression of CD95 ligand on thyroid follicular cells confers immune privilege upon thyroid allografts.

Journal of immunology (Baltimore, Md. : 1950)

Tourneur L,Malassagne B,Batteux F,Fabre M,Mistou S,Lallemand E,Lores P,Chiocchia G

MA5-17858 was used in flow cytometry to report that ectopic FasL expression prevents thyroid allograft rejection.

Wed Aug 01 00:00:00 UTC 2001

Predominance of NK1.1+TCR alpha beta+ or DX5+TCR alpha beta+ T cells in mice conditioned with fractionated lymphoid irradiation protects against graft-versus-host disease: "natural suppressor" cells.

Journal of immunology (Baltimore, Md. : 1950)

Lan F,Zeng D,Higuchi M,Huie P,Higgins JP,Strober S

MA5-17858 was used in flow cytometry to develop a nonmyeloablative host conditioning regimen for transplantation.

Wed Aug 15 00:00:00 UTC 2001

Peritoneal B-cell development depends on strain, radiation, and time.

Experimental hematology

Stickler M,Harding F

MA5-17858 was used in flow cytometry to study generation of peritoneal B-1a cells after bone marrow transfer.

Thu Feb 01 00:00:00 UTC 2001

Surface antigen expression in spleen cells of C57B1/6 mice during ageing: influence of sex and parity.

Clinical and experimental immunology

Barrat F,Lesourd BM,Louise A,Boulouis HJ,Vincent-Naulleau S,Thibault D,Sanaa M,Neway T,Pilet CH

MA5-17858 was used in flow cytometry to test if pregnancies and gender influence the cellar changes observed during ageing in mice.

Sat Mar 01 00:00:00 UTC 1997

生物信息学

蛋白别名: CD11 antigen-like family member B; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; integrin alpha-M; leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; MGC117044

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基因别名: Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1

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Entrez Gene ID: (Mouse) 16409

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功能

opsonin binding glycoprotein binding heparin binding heparan sulfate proteoglycan binding metal ion binding protein heterodimerization activity integrin

参与通路

leukocyte migration involved in inflammatory response cell adhesion leukocyte cell-cell adhesion integrin-mediated signaling pathway microglia development single organismal cell-cell adhesion neutrophil chemotaxis cellular extravasation activated T cell proliferation