Invitrogen

CD11b Monoclonal Antibody (M1/70), PE

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CD11b Antibody (MA1-10082) in Flow

Separation of murine CD11b positive myeloid cells (red-filled) from mouse lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of mouse peritoneum fluid cell suspension stained using anti-mouse CD11b (M1/70) PE Monoclonal antibody (Product # MA1-10082. Concentration in sample 0.1 µg/mL.

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产品信息

MA1-10082

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1-2 µg/mL

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其他PubMed (MISC)

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产品规格

种属反应

Human, Mouse, Non-human primate, Rabbit

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

M1/70

抗原

B10 mouse spleen cells enriched for T cells

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偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Size-exclusion chromatography

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11154207

产品详细信息

This antibody detects an extracellular epitope of CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.

靶标信息

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Human placental mesenchymal stem cells regulate inflammation via the NF‑κB signaling pathway.

Experimental and therapeutic medicine

Liu Y,Zhang X,Hu Y,Kang M,Wu Y,Wang Y,Deng C

MA1-10082 was used in Flow Cytometry experiments to indicate that HPL-MSCs may act as a novel target for the treatment of inflammation-related diseases.

Tue Nov 01 00:00:00 UTC 2022

PML-RARA requires DNA methyltransferase 3A to initiate acute promyelocytic leukemia.

The Journal of clinical investigation

Cole CB,Verdoni AM,Ketkar S,Leight ER,Russler-Germain DA,Lamprecht TL,Demeter RT,Magrini V,Ley TJ

MA110082 was used in flow cytometry to study how DNMT3A is involved in the function of PML-RARA, RUNX1-RUNX1T1, and MLL-AF9

Fri Jan 01 00:00:00 UTC 2016

The RNA-binding protein TTP is a global post-transcriptional regulator of feedback control in inflammation.

Nucleic acids research

Tiedje C,Diaz-Muñoz MD,Trulley P,Ahlfors H,Laaß K,Blackshear PJ,Turner M,Gaestel M

MA1-10082 was used in Flow cytometry/Cell sorting to uncover a role of TTP as a suppressor of feedback inhibitors of inflammation and highlight the importance of TTP activity-regulation by MK2 to control the pro-inflammatory response.

Tue Sep 06 00:00:00 UTC 2016

Type I IFNs induce anti-tumor polarization of tumor associated neutrophils in mice and human.

International journal of cancer

Andzinski L,Kasnitz N,Stahnke S,Wu CF,Gereke M,von Köckritz-Blickwede M,Schilling B,Brandau S,Weiss S,Jablonska J

MA110082 was used in flow cytometry to study the effects of IFN signaling on neutrophil polarization

Fri Apr 15 00:00:00 UTC 2016

Exposure to p,p'-DDE Alters Macrophage Reactivity and Increases Macrophage Numbers in Adipose Stromal Vascular Fraction.

Toxicological sciences : an official journal of the Society of Toxicology

Mangum LH,Crow JA,Stokes JV,Howell GE,Ross MK,Pruett SB,Chambers JE

MA110082 was used in flow cytometry to test if DDE perturbs adipose tissue homeostasis through modulation of macrophage function

Tue Mar 01 00:00:00 UTC 2016

Interleukin-10 From Marginal Zone Precursor B-Cell Subset Is Required for Costimulatory Blockade-Induced Transplantation Tolerance.

Transplantation

Lal G,Nakayama Y,Sethi A,Singh AK,Burrell BE,Kulkarni N,Brinkman CC,Iwami D,Zhang T,Bromberg JS

MA110082 was used in flow cytometry to determine if specific B cell subsets or if B cell-derived interleukin-10 contributes to tolerance

Tue Sep 01 00:00:00 UTC 2015

Chitin induces accumulation in tissue of innate immune cells associated with allergy.

Nature

Reese TA,Liang HE,Tager AM,Luster AD,Van Rooijen N,Voehringer D,Locksley RM

MA1-10082 was used in flow cytometry to characterize the effects of chitin on immune responses

Thu May 03 00:00:00 UTC 2007

Suppressor of cytokine signaling-1 regulates acute inflammatory arthritis and T cell activation.

The Journal of clinical investigation

Egan PJ,Lawlor KE,Alexander WS,Wicks IP

MA1-10082 was used in flow cytometry to examine the role of SOCS-1 in regulating inflammatory and immune responses in acute inflammatory arthritis.

Sat Mar 01 00:00:00 UTC 2003

Ligand-activated natural killer T lymphocytes promptly produce IL-3 and GM-CSF in vivo: relevance to peripheral myeloid recruitment.

European journal of immunology

Leite-de-Moraes MC,Lisbonne M,Arnould A,Machavoine F,Herbelin A,Dy M,Schneider E

MA1-10082 was used in flow cytometry to report that activated NKT cells recruit myeloid progenitors and granulocytes to the periphery through their production of hematopoietic growth factors.

Mon Jul 01 00:00:00 UTC 2002

Immature multipotent hemopoietic progenitors lacking long-term bone marrow-reconstituting activity in the aorta-gonad-mesonephros region of murine day 10 fetuses.

Journal of immunology (Baltimore, Md. : 1950)

Ohmura K,Kawamoto H,Lu M,Ikawa T,Ozaki S,Nakao K,Katsura Y

MA1-10082 was used in flow cytometry to examine multipotent progenitors from early fetuses.

Thu Mar 01 00:00:00 UTC 2001

A peptide derived from bovine beta-casein modulates functional properties of bone marrow-derived macrophages from germfree and human flora-associated mice.

The Journal of nutrition

Sandré C,Gleizes A,Forestier F,Gorges-Kergot R,Chilmonczyk S,Léonil J,Moreau MC,Labarre C

MA1-10082 was used in flow cytometry to report that beta-CN peptide enhances antimicrobial activity of macrophages without proinflammatory effects.

Thu Nov 01 00:00:00 UTC 2001

Emergence of T cell progenitors without B cell or myeloid differentiation potential at the earliest stage of hematopoiesis in the murine fetal liver.

Journal of immunology (Baltimore, Md. : 1950)

Kawamoto H,Ohmura K,Fujimoto S,Katsura Y

MA1-10082 was used in flow cytometry to assess if progenitors colonizing the thymus are T cell lineage restricted.

Mon Mar 01 00:00:00 UTC 1999

A developmental pathway involving four phenotypically and functionally distinct subsets of CD3-CD4-CD8- triple-negative adult mouse thymocytes defined by CD44 and CD25 expression.

Journal of immunology (Baltimore, Md. : 1950)

Godfrey DI,Kennedy J,Suda T,Zlotnik A

MA1-10082 was used in flow cytometry to use CD44 and CD25 to create T cell subsets and then characterize them.

Sat May 15 00:00:00 UTC 1993

生物信息学

蛋白别名: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; integrin alpha M; Integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); Leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; macrophage antigen alpha polypeptide; MGC117044; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit

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基因别名: CD11B; CD11b/CD18; CR3; CR3A; F730045J24Rik; ITGAM; Ly-40; MAC-1; Mac-1a; MAC1; MAC1A; MO1A; SLEB6

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UniProt ID: (Human) P11215

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Entrez Gene ID: (Rabbit) 100351865, (Human) 3684, (Mouse) 16409

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功能

glycoprotein binding protein binding metal ion binding opsonin binding heparin binding heparan sulfate proteoglycan binding protein heterodimerization activity

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