Invitrogen

CD122 Monoclonal Antibody (TM-b1 (TM-beta1)), eBioscience™

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CD122 Antibody (14-1222-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and 0.25 µg of Rat IgG2b K Isotype Control Purified (Product # 14-4031-82) (left) or 0.5 µg of Anti-Mouse CD122 Purified (right) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

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产品信息

14-1222-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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免疫沉淀 (IP)

Assay-Dependent

中和实验 (Neu)

Assay-Dependent

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功能检测 (Functional)

Assay-Dependent

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

TM-b1 (TM-beta1)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_467447

产品详细信息

Description: The TM-b1 monoclonal antibody reacts with mouse CD122, the 90-110 kDa interleukin-2 receptor beta chain (IL-2R beta). CD122 is expressed by NK cells and some T cells in the periphery, and is upregulated by activation. CD122 associates with CD25 and CD132 (common gamma chain) to form the high affinity IL-2 receptor. High affinity binding of IL-2 to the IL-2 receptor is inhibited by the TM-b1 antibody.

Applications Reported: The TM-b1 antibody has been reported for use in flow cytometric analysis, cytokine neutralization, and immunoprecipitation.

Applications Tested: The TM-beta1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD122 (Interleukin 2 receptor) is involved in T cell-mediated immune responses, primarily expressed in the hematopoietic system and is present in 3 forms. The low affinity form of CD122 is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from CD122. The protein encoded by the CD122 gene represents the beta subunit and is a type I membrane protein. Diseases associate with CD122 protein dysfunction include oligoarticular juvenile idiopathic arthritis and rheumatoid factor negative juvenile idiopathic arthritis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

TBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival.

Nature communications

Zhu L,Xie X,Zhang L,Wang H,Jie Z,Zhou X,Shi J,Zhao S,Zhang B,Cheng X,Sun SC

14-1222 was used in Flow cytometry/Cell sorting to study the mechanism of IL-15 function in autophagy and natural killer cell survival.

Wed Jul 18 00:00:00 UTC 2018

A New Immunosuppressive Molecule Emodin Induces both CD4⁺FoxP3⁺ and CD8⁺CD122⁺ Regulatory T Cells and Suppresses Murine Allograft Rejection.

Frontiers in immunology

Qiu F,Liu H,Liang CL,Nie GD,Dai Z

14-1222 was used in Functional assays to investigate the capacity of emodin to prolong transplant survival in a mouse model and explore the cellular and molecular mechanisms underlying its action.

Mon Aug 10 00:00:00 UTC 2020

IL-1β and IL-23 Promote Extrathymic Commitment of CD27⁺CD122^- γδ T Cells to γδT17 Cells.

Journal of immunology (Baltimore, Md. : 1950)

Muschaweckh A,Petermann F,Korn T

14-1222 was used in Flow cytometry/Cell sorting to study the mechanisms by which the peripheral pool of γδ T cells retain a considerable degree of plasticity.

Sun Oct 15 00:00:00 UTC 2017

Blood Stem Cell Activity Is Arrested by Th1-Mediated Injury Preventing Engraftment following Nonmyeloablative Conditioning.

Journal of immunology (Baltimore, Md. : 1950)

Müller AM,Florek M,Kohrt HE,Küpper NJ,Filatenkov A,Linderman JA,Hadeiba H,Negrin RS,Shizuru JA

14-1222 was used in Flow cytometry/Cell sorting to shed new light on the effects of CD4+ T cells on HSC biology.

Tue Nov 15 00:00:00 UTC 2016

T-box Transcription Factors Combine with the Cytokines TGF-β and IL-15 to Control Tissue-Resident Memory T Cell Fate.

Immunity

Mackay LK,Wynne-Jones E,Freestone D,Pellicci DG,Mielke LA,Newman DM,Braun A,Masson F,Kallies A,Belz GT,Carbone FR

14-1222 was used in Flow cytometry/Cell sorting to indicate that the T-box TFs control the two cytokines, TGF-β and IL-15, which are pivotal for CD8(+)CD103(+) Trm cell development and survival.

Tue Dec 15 00:00:00 UTC 2015

Pak2 Links TCR Signaling Strength to the Development of Regulatory T Cells and Maintains Peripheral Tolerance.

Journal of immunology (Baltimore, Md. : 1950)

O'Hagan KL,Choi J,Pryshchep O,Chernoff J,Phee H

14-1222 was used in Flow cytometry/Cell sorting to show a novel role for the actin cytoskeletal remodeling protein, p21-activated kinase 2, in Treg development and homeostasis.

Sat Aug 15 00:00:00 UTC 2015

α-1-Antitrypsin (AAT)-modified donor cells suppress GVHD but enhance the GVL effect: a role for mitochondrial bioenergetics.

Blood

Marcondes AM,Karoopongse E,Lesnikova M,Margineantu D,Welte T,Dinarello CA,Hockenbery D,Janciauskiene S,Deeg HJ

14-1222 was used in Flow cytometry/Cell sorting to investigate the role of α-1-antitrypsin in graft-versus-host disease, showing that α-1-Antitrypsin-modified donor cells suppress graft-versus-host disease but enhance the graft-versus-leukemia effect.

Thu Oct 30 00:00:00 UTC 2014

The transcription factor E4bp4/Nfil3 controls commitment to the NK lineage and directly regulates Eomes and Id2 expression.

The Journal of experimental medicine

Male V,Nisoli I,Kostrzewski T,Allan DS,Carlyle JR,Lord GM,Wack A,Brady HJ

14-1222 was used in Flow cytometry/Cell sorting to propose that E4bp4 is required for commitment to the NK lineage and can promote NK development by directly regulating the expression of the downstream transcription factors Eomes and Id2.

Mon Apr 07 00:00:00 UTC 2014

Inhibition of the TRAIL death receptor by CMV reveals its importance in NK cell-mediated antiviral defense.

PLoS pathogens

Verma S,Loewendorf A,Wang Q,McDonald B,Redwood A,Benedict CA

14-1222 was used in Flow cytometry/Cell sorting to highlight the critical importance for CMV to have evolved a strategy to inhibit TRAIL-DR signaling to thwart NK-mediated defenses.

Fri Aug 01 00:00:00 UTC 2014

Two separate defects affecting true naive or virtual memory T cell precursors combine to reduce naive T cell responses with aging.

Journal of immunology (Baltimore, Md. : 1950)

Renkema KR,Li G,Wu A,Smithey MJ,Nikolich-Žugich J

14-1222 was used in Flow cytometry/Cell sorting to study the causes and components of the age-related erosion of naive T cell responses.

Wed Jan 01 00:00:00 UTC 2014

Cutaneous immunosurveillance and regulation of inflammation by group 2 innate lymphoid cells.

Nature immunology

Roediger B,Kyle R,Yip KH,Sumaria N,Guy TV,Kim BS,Mitchell AJ,Tay SS,Jain R,Forbes-Blom E,Chen X,Tong PL,Bolton HA,Artis D,Paul WE,Fazekas de St Groth B,Grimbaldeston MA,Le Gros G,Weninger W

14-1222 was used in Flow cytometry/Cell sorting to report the identification in normal mouse dermis of an abundant, phenotypically unique group 2 innate lymphoid cell subset that depended on interleukin 7 and constitutively produced IL-13.

Sat Jun 01 00:00:00 UTC 2013

Different NK cell developmental events require different levels of IL-15 trans-presentation.

Journal of immunology (Baltimore, Md. : 1950)

Lee GA,Liou YH,Wang SW,Ko KL,Jiang ST,Liao NS

14-1222 was used in Blocking experiments to demonstrate that different levels of IL-15 trans-presentation are required for different NK cell developmental events.

Mon Aug 01 00:00:00 UTC 2011

生物信息学

蛋白别名: CD122; High affinity IL-2 receptor subunit beta; IL-15 receptor beta chain; IL-2 receptor beta chain; IL-2 receptor subunit beta; IL-2/15 receptor-beta; IL-2R subunit beta; IL-2RB; Interleukin-2 receptor subunit beta; p70-75

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基因别名: CD122; IL-15Rbeta; Il-2/15Rbeta; Il-2Rbeta; IL15Rbeta; Il2rb; p70

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UniProt ID: (Mouse) P16297

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Entrez Gene ID: (Mouse) 16185

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功能

cytokine receptor activity interleukin-2 receptor activity interleukin-2 binding transmembrane signal receptor

参与通路

cytokine-mediated signaling pathway natural killer cell activation interleukin-2-mediated signaling pathway negative regulation of apoptotic process