Invitrogen

CD13 Monoclonal Antibody (WM-15 (WM15)), FITC, eBioscience™

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CD13 Antibody (11-0138-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD13 FITC (purple histogram). Cells in the granulocyte gate were used for analysis.

CD13 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

CD13 Antibody in Immunocytochemistry (ICC/IF)

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产品信息

11-0138-42

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

5 µL (1 µg)/test

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

WM-15 (WM15)

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11043278

产品详细信息

Description: The WM-15 monoclonal antibody reacts with human CD13, also known as aminopeptidase N. CD13 is a transmembrane ectoenzyme occurring on monocytes and granulocytes but not lymphocytes in blood. It is also reportedly on a wide variety of cells and in a wide variety of tissues, including endothelial cells, epithelial cells, fibroblasts, kidney proximal tubules, intestine, and placenta. CD13 is reported to have many different functions including degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and adhesion and migration of cells.

Applications Reported: The WM-15 (WM15) antibody) has been reported for use in flow cytometric analysis.

Applications Tested: This WM-15 (WM15) antibody has been pre-titrated and tested by flow cytometric analysis of lysed whole blood. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD13 (ANPEP) is a 150-170 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. CD13 is a metalloprotease that preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 also has a role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is expressed by granulocytes, monocytes, myeloid leukemia cells, and is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas. CD13 functions as an aminopeptidase enzyme and is also alpha receptor for coronavirus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

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Journal for immunotherapy of cancer

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Published figure using CD13 monoclonal antibody (Product # 11-0138-42) in Flow Cytometry

Wed Mar 01 00:00:00 UTC 2023

JIB-04, a Pan-Inhibitor of Histone Demethylases, Targets Histone-Lysine-Demethylase-Dependent AKT Pathway, Leading to Cell Cycle Arrest and Inhibition of Cancer Stem-Like Cell Properties in Hepatocellular Carcinoma Cells.

International journal of molecular sciences

Lee J,Kim JS,Cho HI,Jo SR,Jang YK

11-0138-42 was used in Flow cytometry/Cell sorting to suggest that the inhibitory effect of JIB-04 on HCC malignancy and the maintenance of LCSCs is mediated via targeting the KDM6B-AKT2 pathway, indicating the therapeutic potential of JIB-04.

Mon Jul 11 00:00:00 UTC 2022

miR-378-3p Knockdown Recapitulates Many of the Features of Myelodysplastic Syndromes.

The American journal of pathology

Wang D,Lin M,Utz B,Bosompem A,Guo Y,Daneshbod Y,Alford CE,Nettles SA,Scher J,Gagne EY,O'Neill M,Barrow L,Wojciechowska N,Keegan J,Mosse CA,Lederer JA,Kim AS

11-0138-42 was used in Flow Cytometry to exhibit a transcriptional profile indicating aberrant maturation and function, immunophenotypic and morphologic dysplasia, and aberrant growth that characterizes MDS.

Mon Nov 01 00:00:00 UTC 2021

N¹-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Nature communications

Wang Y,Wang J,Li X,Xiong X,Wang J,Zhou Z,Zhu X,Gu Y,Dominissini D,He L,Tian Y,Yi C,Fan Z

11-0138-42 was used in Flow Cytometry, Immunocytochemistry to show that N1-methyladenosine methylation (m1A) in tRNA is remarkably elevated in hepatocellular carcinoma (HCC) patient tumour tissues.

Tue Nov 02 00:00:00 UTC 2021

N¹-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Nature communications

Wang Y,Wang J,Li X,Xiong X,Wang J,Zhou Z,Zhu X,Gu Y,Dominissini D,He L,Tian Y,Yi C,Fan Z

11-0138-42 was used in Flow Cytometry, Immunocytochemistry to show that N1-methyladenosine methylation (m1A) in tRNA is remarkably elevated in hepatocellular carcinoma (HCC) patient tumour tissues.

Tue Nov 02 00:00:00 UTC 2021

A Small Molecule Inhibitor, OGP46, Is Effective against Imatinib-Resistant BCR-ABL Mutations via the BCR-ABL/JAK-STAT Pathway.

Molecular therapy oncolytics

Wei L,Yang Y,Gupta P,Wang A,Zhao M,Zhao Y,Qu M,Ke Y,Liu Y,Liu HM,Xu X,Sun Y,Chen ZS,Hu Z

11-0138 was used in Flow cytometry/Cell sorting to highlight that OGP46 is active against not only native BCR-ABL but also 11 clinically relevant BCR-ABL mutations, including T315I mutation, which are resistant to imatinib.

Fri Sep 25 00:00:00 UTC 2020

A Small Molecule Inhibitor, OGP46, Is Effective against Imatinib-Resistant BCR-ABL Mutations via the BCR-ABL/JAK-STAT Pathway.

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Wei L,Yang Y,Gupta P,Wang A,Zhao M,Zhao Y,Qu M,Ke Y,Liu Y,Liu HM,Xu X,Sun Y,Chen ZS,Hu Z

Fri Sep 25 00:00:00 UTC 2020

A Small Molecule Inhibitor, OGP46, Is Effective against Imatinib-Resistant BCR-ABL Mutations via the BCR-ABL/JAK-STAT Pathway.

Molecular therapy oncolytics

Wei L,Yang Y,Gupta P,Wang A,Zhao M,Zhao Y,Qu M,Ke Y,Liu Y,Liu HM,Xu X,Sun Y,Chen ZS,Hu Z

Fri Sep 25 00:00:00 UTC 2020

SRPK1 maintains acute myeloid leukemia through effects on isoform usage of epigenetic regulators including BRD4.

Nature communications

Tzelepis K,De Braekeleer E,Aspris D,Barbieri I,Vijayabaskar MS,Liu WH,Gozdecka M,Metzakopian E,Toop HD,Dudek M,Robson SC,Hermida-Prado F,Yang YH,Babaei-Jadidi R,Garyfallos DA,Ponstingl H,Dias JML,Gallipoli P,Seiler M,Buonamici S,Vick B,Bannister AJ,Rad R,Prinjha RK,Marioni JC,Huntly B,Batson J,Morris JC,Pina C,Bradley A,Jeremias I,Bates DO,Yusa K,Kouzarides T,Vassiliou GS

11-0138 was used in Flow cytometry/Cell sorting to demonstrate that genetic/pharmacological inhibition of SRPK1 leads to cell cycle arrest, leukaemic cell differentiation and prolonged survival of mice transplanted with MLL-rearranged AML.

Wed Dec 19 00:00:00 UTC 2018

Wogonin reversed resistant human myelogenous leukemia cells via inhibiting Nrf2 signaling by Stat3/NF-κB inactivation.

Scientific reports

Xu X,Zhang X,Zhang Y,Yang L,Liu Y,Huang S,Lu L,Kong L,Li Z,Guo Q,Zhao L

11-0138 was used in Flow cytometry/Cell sorting to investigate the mechanism of reduction of Nrf2 mRNA by Wogonin.

Thu Feb 02 00:00:00 UTC 2017

LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.

Nature communications

Zhu P,Wang Y,Wu J,Huang G,Liu B,Ye B,Du Y,Gao G,Tian Y,He L,Fan Z

11-0138 was used in Immunofluorescence to investigate the biology of hepatic cancer stem cells.

Thu Dec 01 00:00:00 UTC 2016

ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells.

The Journal of clinical investigation

Zhu P,Wang Y,He L,Huang G,Du Y,Zhang G,Yan X,Xia P,Ye B,Wang S,Hao L,Wu J,Fan Z

11-0138 was used in Flow cytometry/Cell sorting to investigate the biology of hepatic cancer stem cells.

Thu Oct 01 00:00:00 UTC 2015

ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells.

The Journal of clinical investigation

Zhu P,Wang Y,He L,Huang G,Du Y,Zhang G,Yan X,Xia P,Ye B,Wang S,Hao L,Wu J,Fan Z

11-0138 was used in Flow cytometry/Cell sorting to investigate the biology of hepatic cancer stem cells.

Thu Oct 01 00:00:00 UTC 2015

生物信息学

蛋白别名: alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; Aminopeptidase M; Aminopeptidase N; AP-M; AP-N; CD13; gp150; h AP-N; hAPN; membrane alanyl aminopeptidase; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13

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基因别名: ANPEP; APN; CD13; GP150; LAP1; P150; PEPN

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UniProt ID: (Human) P15144

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Entrez Gene ID: (Human) 290

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功能

virus receptor activity aminopeptidase activity receptor activity metallopeptidase activity zinc ion binding peptide binding metalloaminopeptidase activity metalloprotease

参与通路

angiogenesis proteolysis cellular aromatic compound metabolic process cell differentiation negative regulation of renal sodium excretion peptide catabolic process viral entry into host cell