Invitrogen

CD133 Polyclonal Antibody

CD133 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

CD133 Antibody (PA5-38014) in ICC/IF

Immunocytochemistry of PROM1 in Jurkat cells with CD133 Polyclonal Antibody (Product # PA5-38014) at 2.5 µg/mL.

Product Image Gallery

产品信息

PA5-38014

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

2.5 µg/mL

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

查看2篇已发表文章 2篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

18 amino acid peptide near the carboxy terminus of human PROM1

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2554618

产品详细信息

A recommended positive control is Jurkat cell lysate.

Multiple isoforms of PROM1 are known to exist.

靶标信息

The CD133 (AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. CD133 is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. CD133 has been shown to be expressed in cancers, including some leukemias and brain tumors, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of the CD133 gene is also associated with several types of cancer. CD133 is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner, and multiple transcript variants encoding different isoforms have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Exosome-mediated inhibition of microRNA-449a promotes the amplification of mouse retinal progenitor cells and enhances their transplantation in retinal degeneration mouse models.

Molecular therapy. Nucleic acids

Guo CJ,Cao XL,Zhang YF,Yue KY,Han J,Yan H,Han H,Zheng MH

PA5-38014 was used in Western Blotting to show that exosome-miR-449a inhibitor can effectively promote the expansion of RPCs in vitro and enhance transplanted RPCs survival in vivo, which might provide a novel intervention strategy for retinal degenerations in the future.

Tue Mar 14 00:00:00 UTC 2023

Exosome-mediated inhibition of microRNA-449a promotes the amplification of mouse retinal progenitor cells and enhances their transplantation in retinal degeneration mouse models.

Molecular therapy. Nucleic acids

Guo CJ,Cao XL,Zhang YF,Yue KY,Han J,Yan H,Han H,Zheng MH

Tue Mar 14 00:00:00 UTC 2023

An atlas of healthy and injured cell states and niches in the human kidney.

Nature

Lake BB,Menon R,Winfree S,Hu Q,Melo Ferreira R,Kalhor K,Barwinska D,Otto EA,Ferkowicz M,Diep D,Plongthongkum N,Knoten A,Urata S,Mariani LH,Naik AS,Eddy S,Zhang B,Wu Y,Salamon D,Williams JC,Wang X,Balderrama KS,Hoover PJ,Murray E,Marshall JL,Noel T,Vijayan A,Hartman A,Chen F,Waikar SS,Rosas SE,Wilson FP,Palevsky PM,Kiryluk K,Sedor JR,Toto RD,Parikh CR,Kim EH,Satija R,Greka A,Macosko EZ,Kharchenko PV,Gaut JP,Hodgin JB,Eadon MT,Dagher PC,El-Achkar TM,Zhang K,Kretzler M,Jain S

PA5-38014 was used in Immunohistochemistry to define 28 cellular states across nephron segments and interstitium that were altered in kidney injury, encompassing cycling, adaptive (successful or maladaptive repair), transitioning and degenerative states.

Sat Jul 01 00:00:00 UTC 2023

Organ-specific extracellular matrix directs trans-differentiation of mesenchymal stem cells and formation of salivary gland-like organoids in vivo.

Stem cell research & therapy

Tran ON,Wang H,Li S,Malakhov A,Sun Y,Abdul Azees PA,Gonzalez AO,Cao B,Marinkovic M,Singh BB,Dean DD,Yeh CK,Chen XD

PA5-38014 was used in Flow cytometry/Cell sorting to test the hypothesis that multipotent bone marrow-derived mesenchymal stem cells (BM-MSCs) in a rat model are capable of trans-differentiating to the salivary gland epithelial cell lineage when induced by a native SG-specific extracellular matrix (SG-ECM) and thus may be a viable substitute for repairing damaged SGs.

Fri Jul 15 00:00:00 UTC 2022

Organ-specific extracellular matrix directs trans-differentiation of mesenchymal stem cells and formation of salivary gland-like organoids in vivo.

Stem cell research & therapy

Tran ON,Wang H,Li S,Malakhov A,Sun Y,Abdul Azees PA,Gonzalez AO,Cao B,Marinkovic M,Singh BB,Dean DD,Yeh CK,Chen XD

Fri Jul 15 00:00:00 UTC 2022

Alkaloids of fascaplysin are effective conventional chemotherapeutic drugs, inhibiting the proliferation of C6 glioma cells and causing their death in vitro.

Oncology letters

Bryukhovetskiy I,Lyakhova I,Mischenko P,Milkina E,Zaitsev S,Khotimchenko Y,Bryukhovetskiy A,Polevshchikov A,Kudryavtsev I,Khotimchenko M,Zhidkov M

PA538014 was used in immunocytochemistry to compare the efficiency of fascaplysin with other drugs used to treat glial tumors

Wed Feb 01 00:00:00 UTC 2017

miR-200b inhibits CD133⁺ glioma cells by targeting the AKT pathway.

Oncology letters

Liu A,Yu Q,Peng Z,Huang Y,Diao S,Cheng J,Wang W,Hong M

PA5-38014 was used in Magnetic cell separation to explore the fundamental role of miR-200b and CD133 in glioma stem cell behavior.

Thu Jun 01 00:00:00 UTC 2017

Blockade of IDO-kynurenine-AhR metabolic circuitry abrogates IFN-γ-induced immunologic dormancy of tumor-repopulating cells.

Nature communications

Liu Y,Liang X,Yin X,Lv J,Tang K,Ma J,Ji T,Zhang H,Dong W,Jin X,Chen D,Li Y,Zhang S,Xie HQ,Zhao B,Zhao T,Lu J,Hu ZW,Cao X,Qin FX,Huang B

Published figure using CD133 polyclonal antibody (Product # PA5-38014) in Flow Cytometry

Wed May 10 00:00:00 UTC 2017

LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4.

Journal of experimental & clinical cancer research : CR

Han S,Wang C,Qin X,Xia J,Wu A

PA5-38014 was used in Western Blotting to examine the ability of lipopolysaccharise to affect glioma and glioma stem-like cells in vitro and to induce antitumour immunity in vivo and the role of TLR4 in these processes.

Thu Jun 22 00:00:00 UTC 2017

LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4.

Journal of experimental & clinical cancer research : CR

Han S,Wang C,Qin X,Xia J,Wu A

Thu Jun 22 00:00:00 UTC 2017

生物信息学

蛋白别名: Antigen AC133; Antigen AC133 homolog; antigen CD133; CD107b; CD133; fudenine; hematopoietic stem cell antigen; hProminin; Lamp II; LAMP-2; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; LGP-96; LGP-B; Mac3; Prominin-1; prominin-like 1; Prominin-like protein 1; Prominin1

查看更多收起

基因别名: 4932416E19Rik; AC133; CD133; CORD12; MCDR2; MSTP061; Prom; Prom-1; PROM1; PROML1; RP41; STGD4

查看更多收起

UniProt ID: (Human) Q6SV49, (Mouse) O54990

查看更多收起

Entrez Gene ID: (Human) 8842, (Mouse) 19126, (Rat) 60357

查看更多收起

功能

protein binding actinin binding cadherin binding molecular_function

参与通路

retina layer formation photoreceptor cell maintenance retina morphogenesis in camera-type eye camera-type eye photoreceptor cell differentiation glomerular visceral epithelial cell differentiation glomerular parietal epithelial cell differentiation positive regulation of nephron tubule epithelial cell differentiation biological_process