Invitrogen

CD137 (4-1BB) Monoclonal Antibody (17B5), PE, eBioscience™

CD137 (4-1BB) Antibody in Flow Cytometry (Flow)

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CD137 (4-1BB) Antibody (12-1371-82) in Flow

Staining of 3-day unstimulated (left) and 3-day ConA-stimulated (right) BALB/c splenocytes with 0.125 µg of Golden Syrian Hamster IgG Isotype Control PE (Product # 12-4914-81) (open histogram) or 0.125 µg of Anti-Mouse CD137 (4-1BB) PE (filled histogram). Total viable cells were used for analysis.

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产品信息

12-1371-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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功能检测 (Functional)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Syrian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

17B5

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_465864

产品详细信息

Description: The 17B5 monoclonal antibody reacts with mouse CD137, also known as 4-1BB, a member of the TNFR superfamily. CD137 is expressed as monomers, dimers, and tetramers on the surface of activated T cells around day 4-6 after activation through the TCR complex. Interaction of CD137 with its ligand, 4-1BBL, is reported to deliver a costimulatory signal important in the T-APC interaction. Recent data suggests CD317 is expressed on Tregs and may play a role in their proliferation. The monoclonal antibody 17B5 has strong antagonistic effect in in vitro functional assays.

Applications Reported: The 17B5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 17B5 antibody has been tested by flow cytometric analysis of activated mouse splenocytes cultures. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Novel lectin-based chimeric antigen receptors target Gb3-positive tumour cells.

Cellular and molecular life sciences : CMLS

Meléndez AV,Velasco Cárdenas RM,Lagies S,Strietz J,Siukstaite L,Thomas OS,Tomisch J,Weber W,Kammerer B,Römer W,Minguet S

Published figure using CD137 (4-1BB) monoclonal antibody (Product # 12-1371-82) in Flow Cytometry

Mon Sep 12 00:00:00 UTC 2022

STARTRAC analyses of scRNAseq data from tumor models reveal T cell dynamics and therapeutic targets.

The Journal of experimental medicine

Bhatt D,Kang B,Sawant D,Zheng L,Perez K,Huang Z,Sekirov L,Wolak D,Huang JY,Liu X,DeVoss J,Manzanillo PS,Pierce N,Zhang Z,Symons A,Ouyang W

Published figure using CD137 (4-1BB) monoclonal antibody (Product # 12-1371-82) in Flow Cytometry

Mon Jun 07 00:00:00 UTC 2021

PD-1 and LAG-3 blockade improve anti-tumor vaccine efficacy.

Oncoimmunology

Zahm CD,Moseman JE,Delmastro LE,G Mcneel D

Published figure using CD137 (4-1BB) monoclonal antibody (Product # 12-1371-82) in Flow Cytometry

Wed Apr 21 00:00:00 UTC 2021

SLAMF6 deficiency augments tumor killing and skews toward an effector phenotype revealing it as a novel T cell checkpoint.

eLife

Hajaj E,Eisenberg G,Klein S,Frankenburg S,Merims S,Ben David I,Eisenhaure T,Henrickson SE,Villani AC,Hacohen N,Abudi N,Abramovich R,Cohen JE,Peretz T,Veillette A,Lotem M

12-1371-82 was used in Flow Cytometry to support the notion that SLAMF6 is an inhibitory immune receptor whose absence enables powerful CD8+ T cells to eradicate tumors.

Tue Mar 03 00:00:00 UTC 2020

Fas mutation reduces obesity by increasing IL-4 and IL-10 expression and promoting white adipose tissue browning.

Scientific reports

Choi EW,Lee M,Song JW,Kim K,Lee J,Yang J,Lee SH,Kim IY,Choi JH,Seong JK

Published figure using CD137 (4-1BB) monoclonal antibody (Product # 12-1371-82) in Flow Cytometry

Mon Jul 20 00:00:00 UTC 2020

Pulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer.

Cell reports

Choi H,Deng J,Li S,Silk T,Dong L,Brea EJ,Houghton S,Redmond D,Zhong H,Boiarsky J,Akbay EA,Smith PD,Merghoub T,Wong KK,Wolchok JD

Published figure using CD137 (4-1BB) monoclonal antibody (Product # 12-1371-82) in Flow Cytometry

Tue Apr 16 00:00:00 UTC 2019

PD-1 Blockade and CD27 Stimulation Activate Distinct Transcriptional Programs That Synergize for CD8⁺ T-Cell-Driven Antitumor Immunity.

Clinical cancer research : an official journal of the American Association for Cancer Research

Buchan SL,Fallatah M,Thirdborough SM,Taraban VY,Rogel A,Thomas LJ,Penfold CA,He LZ,Curran MA,Keler T,Al-Shamkhani A

12-1371 was used in Flow cytometry/Cell sorting to show that suboptimal T-cell invigoration in cancer patients undergoing treatment with PD-1 checkpoint blockers will be improved by dual PD-1 blockade and CD27 agonism.

Tue May 15 00:00:00 UTC 2018

Ablation of Transcription Factor IRF4 Promotes Transplant Acceptance by Driving Allogenic CD4⁺ T Cell Dysfunction.

Immunity

Wu J,Zhang H,Shi X,Xiao X,Fan Y,Minze LJ,Wang J,Ghobrial RM,Xia J,Sciammas R,Li XC,Chen W

12-1371-82 was used in Flow Cytometry to demonstrate that interferon regulatory factor 4 was a key transcriptional determinant controlling T cell responses during transplantation.

Tue Dec 19 00:00:00 UTC 2017

Anti-CD137 Suppresses Tumor Growth by Blocking Reverse Signaling by CD137 Ligand.

Cancer research

Kang SW,Lee SC,Park SH,Kim J,Kim HH,Lee HW,Seo SK,Kwon BS,Cho HR,Kwon B

12-1371-82 was used in Flow Cytometry to report findings related to reverse signaling by CD137 ligand (CD137L) in antigen-presenting dendritic cells (DC) in tumors that address these paradoxical results.

Wed Nov 01 00:00:00 UTC 2017

Vaccination with High-Affinity Epitopes Impairs Antitumor Efficacy by Increasing PD-1 Expression on CD8⁺ T Cells.

Cancer immunology research

Zahm CD,Colluru VT,McNeel DG

Published figure using CD137 (4-1BB) monoclonal antibody (Product # 12-1371-82) in Flow Cytometry

Tue Aug 01 00:00:00 UTC 2017

Impact of enzymatic tissue disintegration on the level of surface molecule expression and immune cell function.

European journal of microbiology & immunology

Autengruber A,Gereke M,Hansen G,Hennig C,Bruder D

12-1371 was used in Flow cytometry/Cell sorting to indicate that enzymatic tissue disintegration can have profound effects on the expression of a variety of cell-surface molecules.

Fri Jun 01 00:00:00 UTC 2012

CD137 ligand activated microglia induces oligodendrocyte apoptosis via reactive oxygen species.

Journal of neuroinflammation

Yeo YA,Martínez Gómez JM,Croxford JL,Gasser S,Ling EA,Schwarz H

12-1371 was used in Flow cytometry/Cell sorting to show the activating effects of CD137L signaling to microglia,and show that the CD137 system may be a mediator of neuroinflammatory and neurodegenerative disease.

Mon Jul 16 00:00:00 UTC 2012

Reduced Treg frequency in LFA-1-deficient mice allows enhanced T effector differentiation and pathology in EAE.

European journal of immunology

Gültner S,Kuhlmann T,Hesse A,Weber JP,Riemer C,Baier M,Hutloff A

12-1371 was used in Flow cytometry/Cell sorting to suggest a substantial role of LFA-1 in Treg generation and subsequent expansion of effector T cells and highlight the importance of Treg in limiting EAE.

Wed Dec 01 00:00:00 UTC 2010

Regulation of granulocyte and macrophage populations of murine bone marrow cells by G-CSF and CD137 protein.

PloS one

Jiang D,Schwarz H

12-1371 was used in Flow cytometry/Cell sorting to confirm the role of CD137 ligand signalling in regulating myelopoiesis, and demonstrate that this growth promoting influence is restricted to the monocytic lineage.

Mon Dec 13 00:00:00 UTC 2010

Pancreatic lipase-related protein 2 (PLRP2) induction by IL-4 in cytotoxic T lymphocytes (CTLs) and reevaluation of the negative effects of its gene ablation on cytotoxicity.

Journal of leukocyte biology

Alves BN,Leong J,Tamang DL,Elliott V,Edelnant J,Redelman D,Singer CA,Kuhn AR,Miller R,Lowe ME,Hudig D

12-1371 was used in Flow cytometry/Cell sorting to study how extracellular PLRP2 lipase is unable to increase the cytotoxicity lost through PLRP2 ablation, and the overall role of PLRP2 in CD8 T cells..

Tue Sep 01 00:00:00 UTC 2009

The 4-1BB costimulation augments the proliferation of CD4+CD25+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Zheng G,Wang B,Chen A

Sun Aug 15 00:00:00 UTC 2004

生物信息学

蛋白别名: 4 1 BB; 4-1BB ligand receptor; 41BB; CD137; CD137 antigen; s4 1 BB; s4 1BB; s41BB; sCD137; secreted CD137 antigen; soluble 4 1 BB; soluble 4 1BB; soluble 41BB; soluble CD137; T-cell antigen 4-1BB; Tumor necrosis factor receptor superfamily member 9

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基因别名: 4-1BB; A930040I11Rik; AA408498; AI325004; Cd137; CDw137; ILA; Ly63; Tnfrsf9

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UniProt ID: (Mouse) P20334

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Entrez Gene ID: (Mouse) 21942

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功能

receptor activity cytokine binding transmembrane signal receptor

参与通路

regulation of cell proliferation protein homotrimerization negative regulation of interleukin-10 secretion negative regulation of interleukin-12 secretion