Invitrogen

CD137 (4-1BB) Monoclonal Antibody (17B5), Biotin, eBioscience™

CD137 (4-1BB) Antibody in Flow Cytometry (Flow)

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CD137 (4-1BB) Antibody (13-1371-82) in Flow

Staining of ConA-stimulated mouse splenocytes with Anti-Mouse CD137 (4-1BB) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

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产品信息

13-1371-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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功能检测 (Functional)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Syrian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

17B5

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_466604

产品详细信息

Description: The 17B5 monoclonal antibody reacts with mouse CD137, also known as 4-1BB, a member of the TNFR superfamily. CD137 is expressed as monomers, dimers, and tetramers on the surface of activated T cells around day 4-6 after activation through the TCR complex. Interaction of CD137 with its ligand, 4-1BBL, is reported to deliver a costimulatory signal important in the T-APC interaction. Recent data suggests CD317 is expressed on Tregs and may play a role in their proliferation. The monoclonal antibody 17B5 has strong antagonistic effect in in vitro functional assays.

Applications Reported: The 17B5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 17B5 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Genetic deletion of HVEM in a leukemia B cell line promotes a preferential increase of PD-1^- stem cell-like T cells over PD-1⁺ T cells curbing tumor progression.

Frontiers in immunology

Del Rio ML,de Juan CY,Roncador G,Caleiras E,Álvarez-Esteban R,Pérez-Simón JA,Rodriguez-Barbosa JI

13-1371-82 was used in Flow cytometry/Cell sorting to highlight the role of co-inhibitory signals delivered by Herpes Virus Entry Mediator (HVEM, TNFRSF14) upon engagement of BTLA on T cells and NK cells, placing HVEM/BTLA interaction in the spotlight as a novel immune checkpoint for the reinforcement of the anti-tumor responses in malignancies of hematopoietic origin.

Thu Aug 10 00:00:00 UTC 2023

Novel lectin-based chimeric antigen receptors target Gb3-positive tumour cells.

Cellular and molecular life sciences : CMLS

Meléndez AV,Velasco Cárdenas RM,Lagies S,Strietz J,Siukstaite L,Thomas OS,Tomisch J,Weber W,Kammerer B,Römer W,Minguet S

Published figure using CD137 (4-1BB) monoclonal antibody (Product # 13-1371-82) in Flow Cytometry

Mon Sep 12 00:00:00 UTC 2022

STARTRAC analyses of scRNAseq data from tumor models reveal T cell dynamics and therapeutic targets.

The Journal of experimental medicine

Bhatt D,Kang B,Sawant D,Zheng L,Perez K,Huang Z,Sekirov L,Wolak D,Huang JY,Liu X,DeVoss J,Manzanillo PS,Pierce N,Zhang Z,Symons A,Ouyang W

Published figure using CD137 (4-1BB) monoclonal antibody (Product # 13-1371-82) in Flow Cytometry

Mon Jun 07 00:00:00 UTC 2021

PD-1 and LAG-3 blockade improve anti-tumor vaccine efficacy.

Oncoimmunology

Zahm CD,Moseman JE,Delmastro LE,G Mcneel D

Published figure using CD137 (4-1BB) monoclonal antibody (Product # 13-1371-82) in Flow Cytometry

Wed Apr 21 00:00:00 UTC 2021

Fas mutation reduces obesity by increasing IL-4 and IL-10 expression and promoting white adipose tissue browning.

Scientific reports

Choi EW,Lee M,Song JW,Kim K,Lee J,Yang J,Lee SH,Kim IY,Choi JH,Seong JK

Published figure using CD137 (4-1BB) monoclonal antibody (Product # 13-1371-82) in Flow Cytometry

Mon Jul 20 00:00:00 UTC 2020

Pulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer.

Cell reports

Choi H,Deng J,Li S,Silk T,Dong L,Brea EJ,Houghton S,Redmond D,Zhong H,Boiarsky J,Akbay EA,Smith PD,Merghoub T,Wong KK,Wolchok JD

Published figure using CD137 (4-1BB) monoclonal antibody (Product # 13-1371-82) in Flow Cytometry

Tue Apr 16 00:00:00 UTC 2019

Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies.

Cancer cell

Arce Vargas F,Furness AJS,Litchfield K,Joshi K,Rosenthal R,Ghorani E,Solomon I,Lesko MH,Ruef N,Roddie C,Henry JY,Spain L,Ben Aissa A,Georgiou A,Wong YNS,Smith M,Strauss D,Hayes A,Nicol D,O'Brien T,Mårtensson L,Ljungars A,Teige I,Frendéus B,Pule M,Marafioti T,Gore M,Larkin J,Turajlic S,Swanton C,Peggs KS,Quezada SA

13-1371 was used in Flow cytometry/Cell sorting to examine the importance of Fc-gamma receptor binding in contributing to the activity of anti-CTLA4 antibodies.

Mon Apr 09 00:00:00 UTC 2018

Vaccination with High-Affinity Epitopes Impairs Antitumor Efficacy by Increasing PD-1 Expression on CD8⁺ T Cells.

Cancer immunology research

Zahm CD,Colluru VT,McNeel DG

Published figure using CD137 (4-1BB) monoclonal antibody (Product # 13-1371-82) in Flow Cytometry

Tue Aug 01 00:00:00 UTC 2017

Concomitant targeting of programmed death-1 (PD-1) and CD137 improves the efficacy of radiotherapy in a mouse model of human BRAFV600-mutant melanoma.

Cancer immunology, immunotherapy : CII

Kroon P,Gadiot J,Peeters M,Gasparini A,Deken MA,Yagita H,Verheij M,Borst J,Blank CU,Verbrugge I

13-1371 was used in Flow cytometry/Cell sorting to examine the extent to which T cell co-stimulation and/or stereotactic body radiation therapy could enhance the therapeutic efficacy of T cell checkpoint blockade.

Wed Jun 01 00:00:00 UTC 2016

4-1BB ligand signaling to T cells limits T cell activation.

Journal of immunology (Baltimore, Md. : 1950)

Eun SY,Lee SW,Xu Y,Croft M

13-1371 was used in Flow cytometry/Cell sorting to show that 4-1BBL can transmit signals that limit T cell effector activity under tolerogenic conditions.

Thu Jan 01 00:00:00 UTC 2015

Tissues in different anatomical sites can sculpt and vary the tumor microenvironment to affect responses to therapy.

Molecular therapy : the journal of the American Society of Gene Therapy

Devaud C,Westwood JA,John LB,Flynn JK,Paquet-Fifield S,Duong CP,Yong CS,Pegram HJ,Stacker SA,Achen MG,Stewart TJ,Snyder LA,Teng MW,Smyth MJ,Darcy PK,Kershaw MH

13-1371 was used in Flow cytometry/Cell sorting to explore the relationship between tumour microenvironments and efficacy of immunotherapy.

Wed Jan 01 00:00:00 UTC 2014

The 4-1BB costimulation augments the proliferation of CD4+CD25+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Zheng G,Wang B,Chen A

Sun Aug 15 00:00:00 UTC 2004

生物信息学

蛋白别名: 4 1 BB; 4-1BB ligand receptor; 41BB; CD137; CD137 antigen; s4 1 BB; s4 1BB; s41BB; sCD137; secreted CD137 antigen; soluble 4 1 BB; soluble 4 1BB; soluble 41BB; soluble CD137; T-cell antigen 4-1BB; Tumor necrosis factor receptor superfamily member 9

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基因别名: 4-1BB; A930040I11Rik; AA408498; AI325004; Cd137; CDw137; ILA; Ly63; Tnfrsf9

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UniProt ID: (Mouse) P20334

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Entrez Gene ID: (Mouse) 21942

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功能

receptor activity cytokine binding transmembrane signal receptor

参与通路

regulation of cell proliferation protein homotrimerization negative regulation of interleukin-10 secretion negative regulation of interleukin-12 secretion