Invitrogen

CD14 Monoclonal Antibody (Sa2-8), PE-Cyanine7, eBioscience™

图: 1 / 28

CD14 Antibody (25-0141-82) in Flow

Staining of mouse thioglycolate-elicited peritoneal exudate cells with 0.5 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (blue histogram) or 0.5 µg of Anti-Mouse CD14 PE-Cyanine7 (purple histogram). Total viable cells were used for analysis.

Product Image Gallery

产品信息

25-0141-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

查看21篇已发表文章 21篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

Sa2-8

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11220077

产品详细信息

Description: The Sa2-8 monoclonal antibody reacts with mouse CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is a receptor for the complexes of LPS and LBP (LPS-Binding Protein) and is shown to associate with Toll-Like Receptor 4 (TLR4) and participate in the signaling and cellular response to bacterial LPS. In mouse, CD14 is expressed on the surface of macrophages and under certain conditions is also found in the serum in a secreted form. Sa2-8 has weak antagonistic activity (in NF-kappaB activation or TNF alpha production with LPS stimulation).

Applications Reported: This Sa2-8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This Sa2-8 antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Fasudil-modified macrophages reduce inflammation and regulate the immune response in experimental autoimmune encephalomyelitis.

Neural regeneration research

Liu C,Guo S,Liu R,Guo M,Wang Q,Chai Z,Xiao B,Ma C

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Fri Mar 01 00:00:00 UTC 2024

Umbilical cord mesenchymal stem cell-derived apoptotic extracellular vesicles ameliorate cutaneous wound healing in type 2 diabetic mice via macrophage pyroptosis inhibition.

Stem cell research & therapy

Wang Y,Jing L,Lei X,Ma Z,Li B,Shi Y,Zhang W,Li Y,Zhou H,Hu K,Xue Y,Jin Y

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Tue Sep 19 00:00:00 UTC 2023

Optimization of differentiation and transcriptomic profile of THP-1 cells into macrophage by PMA.

PloS one

Liu T,Huang T,Li J,Li A,Li C,Huang X,Li D,Wang S,Liang M

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Wed Jul 19 00:00:00 UTC 2023

Macrophage polarization is involved in liver fibrosis induced by β ₁-adrenoceptor autoantibody.

Acta biochimica et biophysica Sinica

Wu Y,Fan X,Yu H,Liu J,Duan Y,Zhang S,Yan L,Du Y,Liu H

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Thu Aug 25 00:00:00 UTC 2022

Genomic and epigenomic adaptation in SP-R210 (Myo18A) isoform-deficient macrophages.

Immunobiology

Yau E,Chen Y,Song C,Webb J,Carillo M,Kawasawa YI,Tang Z,Takahashi Y,Umstead TM,Dovat S,Chroneos ZC

25-0141-82 was used in Flow Cytometry to suggest that the inflammatory differences seen in SP-R210L-deficient macrophages are a result of transcriptional differences that are mediated by epigenetic changes brought about by differential expression of the SP-R210 isoforms.

Mon Nov 01 00:00:00 UTC 2021

Early activation of the cardiac CX3CL1/CX3CR1 axis delays β-adrenergic-induced heart failure.

Scientific reports

Flamant M,Mougenot N,Balse E,Le Fèvre L,Atassi F,Gautier EL,Le Goff W,Keck M,Nadaud S,Combadière C,Boissonnas A,Pavoine C

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Thu Sep 09 00:00:00 UTC 2021

CD14⁺CD16^- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK.

Arthritis research & therapy

Xue J,Xu L,Zhu H,Bai M,Li X,Zhao Z,Zhong H,Cheng G,Li X,Hu F,Su Y

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Mon Sep 21 00:00:00 UTC 2020

CNS Immune Profiling in a Dengue Virus-Infected Immunocompetent Outbred ICR Mice Strain.

Frontiers in cellular and infection microbiology

Shen TJ,Chen CL,Jhan MK,Tseng PC,Lin CF

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Thu Jun 17 00:00:00 UTC 2021

Leucine-rich repeats and calponin homology containing 4 (Lrch4) regulates the innate immune response.

The Journal of biological chemistry

Aloor JJ,Azzam KM,Guardiola JJ,Gowdy KM,Madenspacher JH,Gabor KA,Mueller GA,Lin WC,Lowe JM,Gruzdev A,Henderson MW,Draper DW,Merrick BA,Fessler MB

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Fri Feb 08 00:00:00 UTC 2019

Chanzyme TRPM7 Mediates the Ca²⁺ Influx Essential for Lipopolysaccharide-Induced Toll-Like Receptor 4 Endocytosis and Macrophage Activation.

Immunity

Schappe MS,Szteyn K,Stremska ME,Mendu SK,Downs TK,Seegren PV,Mahoney MA,Dixit S,Krupa JK,Stipes EJ,Rogers JS,Adamson SE,Leitinger N,Desai BN

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Tue Jan 16 00:00:00 UTC 2018

TIM‑4 blockade of KCs combined with exogenous TGF‑β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts.

International journal of molecular medicine

Wu H,Xu X,Li J,Gong J,Li M

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Sun Jul 01 00:00:00 UTC 2018

MicroRNA-10a/b are regulators of myeloid differentiation and acute myeloid leukemia.

Oncology letters

Bi L,Sun L,Jin Z,Zhang S,Shen Z

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Sun Apr 01 00:00:00 UTC 2018

Butyrate upregulates the TLR4 expression and the phosphorylation of MAPKs and NK-κB in colon cancer cell in vitro.

Oncology letters

Xiao T,Wu S,Yan C,Zhao C,Jin H,Yan N,Xu J,Wu Y,Li C,Shao Q,Xia S

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Mon Oct 01 00:00:00 UTC 2018

Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling.

Nature communications

Pal B,Chen Y,Vaillant F,Jamieson P,Gordon L,Rios AC,Wilcox S,Fu N,Liu KH,Jackling FC,Davis MJ,Lindeman GJ,Smyth GK,Visvader JE

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Mon Nov 20 00:00:00 UTC 2017

Distinct Kinase-Independent Role of RIPK3 in CD11c⁺ Mononuclear Phagocytes in Cytokine-Induced Tissue Repair.

Cell reports

Moriwaki K,Balaji S,Bertin J,Gough PJ,Chan FK

25-0141 was used in Flow cytometry/Cell sorting to study the distinct kinase-independent role of RIPK3 in cytokine-induced tissue repair.

Tue Mar 07 00:00:00 UTC 2017

T3 Regulates a Human Macrophage-Derived TSH-β Splice Variant: Implications for Human Bone Biology.

Endocrinology

Baliram R,Latif R,Morshed SA,Zaidi M,Davies TF

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Thu Sep 01 00:00:00 UTC 2016

Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.

Stem cell reports

Mucci A,Kunkiel J,Suzuki T,Brennig S,Glage S,Kühnel MP,Ackermann M,Happle C,Kuhn A,Schambach A,Trapnell BC,Hansen G,Moritz T,Lachmann N

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Tue Aug 09 00:00:00 UTC 2016

Mechanisms of Toll-like Receptor 4 Endocytosis Reveal a Common Immune-Evasion Strategy Used by Pathogenic and Commensal Bacteria.

Immunity

Tan Y,Zanoni I,Cullen TW,Goodman AL,Kagan JC

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Tue Nov 17 00:00:00 UTC 2015

Role of caveolin-3 in lymphocyte activation.

Life sciences

Tran C,Stary CM,Schilling JM,Bentley B,Patel HH,Roth DM

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Thu Jan 15 00:00:00 UTC 2015

SP-R210 (Myo18A) Isoforms as Intrinsic Modulators of Macrophage Priming and Activation.

PloS one

Yang L,Carrillo M,Wu YM,DiAngelo SL,Silveyra P,Umstead TM,Halstead ES,Davies ML,Hu S,Floros J,McCormack FX,Christensen ND,Chroneos ZC

25-0141 was used in Flow cytometry/Cell sorting to demonstrate that SP-R210 isoforms differentially regulate trafficking, expression, and activation of innate immune receptors on macrophages.

Thu Feb 04 00:00:00 UTC 2016

Circadian mechanisms in murine and human bone marrow mesenchymal stem cells following dexamethasone exposure.

Bone

Wu X,Yu G,Parks H,Hebert T,Goh BC,Dietrich MA,Pelled G,Izadpanah R,Gazit D,Bunnell BA,Gimble JM

Published figure using CD14 monoclonal antibody (Product # 25-0141-82) in Flow Cytometry

Thu May 01 00:00:00 UTC 2008

生物信息学

蛋白别名: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

查看更多收起

基因别名: Cd14

查看更多收起

UniProt ID: (Mouse) P10810

查看更多收起

Entrez Gene ID: (Mouse) 12475

查看更多收起

功能

lipopolysaccharide binding lipoteichoic acid binding transmembrane signal receptor

参与通路