Invitrogen

CD14 Monoclonal Antibody (TuK4), PE

图: 1 / 5

CD14 Antibody (MHCD1404) in Flow

Human peripheral blood monocytes stained using R-PE of anti-human CD14 monoclonal antibody (clone Tuk4). The negative control profiles represent unstained cells.

Product Image Gallery

产品信息

MHCD1404

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

查看7篇已发表文章 7篇已发表文章

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human

已发表种属

Dog, Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

TuK4

抗原

Human CD14

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with 10% sucrose, 4mg/mL BSA

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_10373109

产品详细信息

R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.

靶标信息

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

miR-181a Modulation of ERK-MAPK Signaling Sustains DC-SIGN Expression and Limits Activation of Monocyte-Derived Dendritic Cells.

Cell reports

Lim CX,Lee B,Geiger O,Passegger C,Beitzinger M,Romberger J,Stracke A,Högenauer C,Stift A,Stoiber H,Poidinger M,Zebisch A,Meister G,Williams A,Flavell RA,Henao-Mejia J,Strobl H

MHCD1404 was used in Flow cytometry/Cell sorting to demonstrate that high DC-SIGN expression levels and a high activation threshold in monocyte-derived dendritic cells are linked and simultaneously maintained by miR-181a.

Tue Mar 17 00:00:00 UTC 2020

Physical exercise and human adipose-derived mesenchymal stem cells ameliorate motor disturbances in a male rat model of Parkinson's disease.

Journal of neuroscience research

Cucarián JD,Berrío JP,Rodrigues C,Zancan M,Wink MR,de Oliveira A

Published figure using CD14 monoclonal antibody (Product # MHCD1404) in Flow Cytometry

Sun Sep 01 00:00:00 UTC 2019

Eradication of Canine Diffuse Large B-Cell Lymphoma in a Murine Xenograft Model with CD47 Blockade and Anti-CD20.

Cancer immunology research

Weiskopf K,Anderson KL,Ito D,Schnorr PJ,Tomiyasu H,Ring AM,Bloink K,Efe J,Rue S,Lowery D,Barkal A,Prohaska S,McKenna KM,Cornax I,O'Brien TD,O'Sullivan MG,Weissman IL,Modiano JF

MHCD1404-4 was used in Flow cytometry/Cell sorting to demonstrate, using a mouse xenograft model, the effectiveness of combining CD47-blockade with anti-CD20 as treatment against canine lymphoma.

Thu Dec 01 00:00:00 UTC 2016

Analysis of the safety of mesenchymal stromal cells secretome for glioblastoma treatment.

Cytotherapy

Onzi GR,Ledur PF,Hainzenreder LD,Bertoni AP,Silva AO,Lenz G,Wink MR

MHCD1404 was used in flow cytometry to assess the use of human adipose-derived stem cells to treat cancer

Fri Jul 01 00:00:00 UTC 2016

Antibacterial responses by peritoneal macrophages are enhanced following vitamin D supplementation.

PloS one

Bacchetta J,Chun RF,Gales B,Zaritsky JJ,Leroy S,Wesseling-Perry K,Boregaard N,Rastogi A,Salusky IB,Hewison M

MHCD1404 was used in Flow cytometry/Cell sorting to show that vitamin D supplementation in vitro and in vivo promotes innate immune responses that may enhance macrophage antibacterial responses in patients undergoing PD.

Fri Aug 28 00:00:00 UTC 2015

Coexpression of CD14 and CD326 discriminate hepatic precursors in the human fetal liver.

Stem cells and development

Fomin ME,Tai LK,Bárcena A,Muench MO

Published figure using CD14 monoclonal antibody (Product # MHCD1404) in Flow Cytometry

Fri Jul 01 00:00:00 UTC 2011

The endogenous danger signals HSP70 and MICA cooperate in the activation of cytotoxic effector functions of NK cells.

Journal of cellular and molecular medicine

Elsner L,Flügge PF,Lozano J,Muppala V,Eiz-Vesper B,Demiroglu SY,Malzahn D,Herrmann T,Brunner E,Bickeböller H,Multhoff G,Walter L,Dressel R

MHCD1404-4 was used in Flow cytometry/Cell sorting to elucidate how the activity of two stress-inducible immunological danger signals leads to activation and enhanced cytotoxicity of human natural killer cells against tumour cells.

Thu Apr 01 00:00:00 UTC 2010

Preclinical evaluation of carcinoembryonic cell adhesion molecule (CEACAM) 6 as potential therapy target for pancreatic adenocarcinoma.

The Journal of pathology

Strickland LA,Ross J,Williams S,Ross S,Romero M,Spencer S,Erickson R,Sutcliffe J,Verbeke C,Polakis P,van Bruggen N,Koeppen H

MHCD1404 was used in flow cytometry to investigate targeting CEACAM6 for treatment of pancreatic adenocarcinomas.

Wed Jul 01 00:00:00 UTC 2009

Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy.

Journal of immunological methods

Canonico B,Zamai L,Burattini S,Granger V,Mannello F,Gobbi P,Felici C,Falcieri E,Reilly JT,Barnett D,Papa S

MHCD1404 was used in flow cytometry to assess the effects of a TransFix-based stabilization technique on leukocyte scatter characteristics, immunophenotyping, membrane permeability, absolute cell counting and morphology.

Wed Dec 01 00:00:00 UTC 2004

生物信息学

蛋白别名: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

查看更多收起

基因别名: CD14

查看更多收起

UniProt ID: (Human) P08571

查看更多收起

Entrez Gene ID: (Human) 929

查看更多收起

功能

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding transmembrane signal receptor

参与通路