Invitrogen

CD14 Monoclonal Antibody (TuK4), APC

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CD14 Antibody (MHCD1405) in Flow

Human peripheral blood monocytes stained using APC of anti-human CD14 monoclonal antibody (clone Tuk4). The negative control profiles represent unstained cells.

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产品信息

MHCD1405

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

TuK4

抗原

Human CD14

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with sucrose, 4mg/mL BSA

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_10372796

产品详细信息

Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients.

靶标信息

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Characterization of INCB086550: A Potent and Novel Small-Molecule PD-L1 Inhibitor.

Cancer discovery

Koblish HK,Wu L,Wang LS,Liu PCC,Wynn R,Rios-Doria J,Spitz S,Liu H,Volgina A,Zolotarjova N,Kapilashrami K,Behshad E,Covington M,Yang YO,Li J,Diamond S,Soloviev M,O'Hayer K,Rubin S,Kanellopoulou C,Yang G,Rupar M,DiMatteo D,Lin L,Stevens C,Zhang Y,Thekkat P,Geschwindt R,Marando C,Yeleswaram S,Jackson J,Scherle P,Huber R,Yao W,Hollis G

MHCD1405 was used in Flow Cytometry to identify and characterise INCB086550, a novel, oral, small-molecule programmed cell death protein 1 (PD-1) inhibitor.

Thu Jun 02 00:00:00 UTC 2022

A Framework to Identify Antigen-Expanded T Cell Receptor Clusters Within Complex Repertoires.

Frontiers in immunology

Ceglia V,Kelley EJ,Boyle AS,Zurawski S,Mead HL,Harms CE,Blanck JP,Flamar AL,Kirschman JH,Ogongo P,Ernst JD,Levy Y,Zurawski G,Altin JA

MHCD1405 was used in Flow cytometry/Cell sorting to show that clonotypes within each CET respond to the same epitope, and that protein antigens stimulate multiple CETs reactive to constituent peptides.

Fri Feb 04 00:00:00 UTC 2022

BRAF^V600E-induced senescence drives Langerhans cell histiocytosis pathophysiology.

Nature medicine

Bigenwald C,Le Berichel J,Wilk CM,Chakraborty R,Chen ST,Tabachnikova A,Mancusi R,Abhyankar H,Casanova-Acebes M,Laface I,Akturk G,Jobson J,Karoulia Z,Martin JC,Grout J,Rafiei A,Lin H,Manz MG,Baccarini A,Poulikakos PI,Brown BD,Gnjatic S,Lujambio A,McClain KL,Picarsic J,Allen CE,Merad M

MHCD1405 was used in Flow cytometry/Cell sorting to identify senescent cells as a new target for the treatment of Langerhans cell histiocytosis.

Sat May 01 00:00:00 UTC 2021

BRAF^V600E-induced senescence drives Langerhans cell histiocytosis pathophysiology.

Nature medicine

MHCD1405 was used in Flow cytometry/Cell sorting to identify senescent cells as a new target for the treatment of Langerhans cell histiocytosis.

Sat May 01 00:00:00 UTC 2021

AllergoOncology: Generating a canine anticancer IgE against the epidermal growth factor receptor.

The Journal of allergy and clinical immunology

Fazekas-Singer J,Singer J,Ilieva KM,Matz M,Herrmann I,Spillner E,Karagiannis SN,Jensen-Jarolim E

MHCD1405 was used in Flow cytometry/Cell sorting to evaluate the cytotoxic killing potency of can225IgE-l by MPT-1 canine mast cells against A431 and measured specific tumor cell killing.

Sat Sep 01 00:00:00 UTC 2018

Characterization of porous, dexamethasone-releasing polyurethane coatings for glucose sensors.

Acta biomaterialia

Vallejo-Heligon SG,Klitzman B,Reichert WM

MHCD1405 was used in Flow cytometry/Cell sorting to characterise porous, dexamethasone-releasing polyurethane coatings for glucose sensors.

Sat Nov 01 00:00:00 UTC 2014

A microfabricated electrical differential counter for the selective enumeration of CD4+ T lymphocytes.

Lab on a chip

Watkins NN,Sridhar S,Cheng X,Chen GD,Toner M,Rodriguez W,Bashir R

MHCD1405 was used in Flow cytometry/Cell sorting to develop a microfabricated biochip that enumerates CD4+ T lymphocytes from leukocyte populations, with potential applications in quantitative HIV/AIDS diagnostics.

Thu Apr 21 00:00:00 UTC 2011

Inhibition of the Bcl-xL deamidation pathway in myeloproliferative disorders.

The New England journal of medicine

Zhao R,Follows GA,Beer PA,Scott LM,Huntly BJ,Green AR,Alexander DR

MHCD1405 was used in Flow cytometry/Cell sorting to study how BCR-ABL and mutant JAK2 inhibit the Bcl-x(L) deamidation pathway and the apoptotic response to DNA damage in primary cells from patients with CML or polycythemia vera.

Thu Dec 25 00:00:00 UTC 2008

生物信息学

蛋白别名: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

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基因别名: CD14

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UniProt ID: (Human) P08571

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Entrez Gene ID: (Human) 929

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功能

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding transmembrane signal receptor

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