Invitrogen

CD144 (VE-cadherin) Monoclonal Antibody (16B1), Biotin, eBioscience™

CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)

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CD144 (VE-cadherin) Antibody (13-1449-82) in Flow

Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.25 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.125 µg of Anti-Human CD144 (VE-Cadherin) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD144 (VE-cadherin) Antibody in Western Blot (WB)

CD144 (VE-cadherin) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

CD144 (VE-cadherin) Antibody in Immunocytochemistry (ICC/IF)

CD144 (VE-cadherin) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD144 (VE-cadherin) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD144 (VE-cadherin) Antibody in Immunohistochemistry (IHC)

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产品信息

13-1449-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

Assay-Dependent

查看5篇已发表文章 5篇已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看12篇已发表文章 12篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

16B1

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_466611

产品详细信息

Description: The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.

Applications Reported: This 16B1 antibody has been reported for use in flow cytometric analysis, and immunocytochemistry.

Applications Tested: This 16B1 antibody has been tested by flow cytometric analysis of Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Vascular remodelling in idiopathic pulmonary fibrosis patients and its detrimental effect on lung physiology: potential role of endothelial-to-mesenchymal transition.

ERJ open research

Gaikwad AV,Lu W,Dey S,Bhattarai P,Chia C,Larby J,Haug G,Myers S,Jaffar J,Westall G,Singhera GK,Hackett TL,Markos J,Eapen MS,Sohal SS

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunohistochemistry (Paraffin)

Sat Jan 01 00:00:00 UTC 2022

Upregulation of ETV2 Expression Promotes Endothelial Differentiation of Human Dental Pulp Stem Cells.

Cell transplantation

Li J,Zhu Y,Li N,Wu T,Zheng X,Heng BC,Zou D,Xu J

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Tue Dec 21 00:00:00 UTC 2021

Deciphering the Role of Extracellular Vesicles Derived from ZIKV-Infected hcMEC/D3 Cells on the Blood-Brain Barrier System.

Viruses

Fikatas A,Dehairs J,Noppen S,Doijen J,Vanderhoydonc F,Meyen E,Swinnen JV,Pannecouque C,Schols D

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Western Blot

Thu Nov 25 00:00:00 UTC 2021

Endothelial specific YY1 deletion restricts tumor angiogenesis and tumor growth.

Scientific reports

Liu H,Qiu Y,Pei X,Chitteti R,Steiner R,Zhang S,Jin ZG

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunohistochemistry

Tue Nov 24 00:00:00 UTC 2020

Targeting QKI-7 in vivo restores endothelial cell function in diabetes.

Nature communications

Yang C,Eleftheriadou M,Kelaini S,Morrison T,González MV,Caines R,Edwards N,Yacoub A,Edgar K,Moez A,Ivetic A,Zampetaki A,Zeng L,Wilkinson FL,Lois N,Stitt AW,Grieve DJ,Margariti A

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Thu Jul 30 00:00:00 UTC 2020

Vascular Tumor Recapitulated in Endothelial Cells from hiPSCs Engineered to Express the SERPINE1-FOSB Translocation.

Cell reports. Medicine

van IJzendoorn DGP,Salvatori DCF,Cao X,van den Hil F,Briaire-de Bruijn IH,de Jong D,Mei H,Mummery CL,Szuhai K,Bovée JVMG,Orlova VV

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Tue Dec 22 00:00:00 UTC 2020

Angiogenic and Restorative Abilities of Human Mesenchymal Stem Cells Were Reduced Following Treatment With Serum From Diabetes Mellitus Type 2 Patients.

Journal of cellular biochemistry

Rezaie J,Mehranjani MS,Rahbarghazi R,Shariatzadeh MA

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Mon Jan 01 00:00:00 UTC 2018

Enhanced Therapeutic and Long-Term Dynamic Vascularization Effects of Human Pluripotent Stem Cell-Derived Endothelial Cells Encapsulated in a Nanomatrix Gel.

Circulation

Lee SJ,Sohn YD,Andukuri A,Kim S,Byun J,Han JW,Park IH,Jun HW,Yoon YS

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Tue Nov 14 00:00:00 UTC 2017

MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells.

Stem cells translational medicine

Almalki SG,Llamas Valle Y,Agrawal DK

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Mon May 01 00:00:00 UTC 2017

ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells.

Stem cell research & therapy

Almalki SG,Agrawal DK

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Fri May 12 00:00:00 UTC 2017

Challenges in enumeration of CTCs in breast cancer using techniques independent of cytokeratin expression.

PloS one

Castle J,Morris K,Pritchard S,Kirwan CC

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunocytochemistry

Thu Apr 27 00:00:00 UTC 2017

Challenges in enumeration of CTCs in breast cancer using techniques independent of cytokeratin expression.

PloS one

Castle J,Morris K,Pritchard S,Kirwan CC

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunocytochemistry

Thu Apr 27 00:00:00 UTC 2017

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem cell reports

Li Y,Brauer PM,Singh J,Xhiku S,Yoganathan K,Zúñiga-Pflücker JC,Anderson MK

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Tue Sep 12 00:00:00 UTC 2017

Tumourigenic non-small-cell lung cancer mesenchymal circulating tumour cells: a clinical case study.

Annals of oncology : official journal of the European Society for Medical Oncology

Morrow CJ,Trapani F,Metcalf RL,Bertolini G,Hodgkinson CL,Khandelwal G,Kelly P,Galvin M,Carter L,Simpson KL,Williamson S,Wirth C,Simms N,Frankliln L,Frese KK,Rothwell DG,Nonaka D,Miller CJ,Brady G,Blackhall FH,Dive C

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunofluorescence

Wed Jun 01 00:00:00 UTC 2016

Tumourigenic non-small-cell lung cancer mesenchymal circulating tumour cells: a clinical case study.

Annals of oncology : official journal of the European Society for Medical Oncology

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunofluorescence

Wed Jun 01 00:00:00 UTC 2016

Rab11a Mediates Vascular Endothelial-Cadherin Recycling and Controls Endothelial Barrier Function.

Arteriosclerosis, thrombosis, and vascular biology

Yan Z,Wang ZG,Segev N,Hu S,Minshall RD,Dull RO,Zhang M,Malik AB,Hu G

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Mon Feb 01 00:00:00 UTC 2016

Rab11a Mediates Vascular Endothelial-Cadherin Recycling and Controls Endothelial Barrier Function.

Arteriosclerosis, thrombosis, and vascular biology

Yan Z,Wang ZG,Segev N,Hu S,Minshall RD,Dull RO,Zhang M,Malik AB,Hu G

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Mon Feb 01 00:00:00 UTC 2016

Vasculogenic mimicry in small cell lung cancer.

Nature communications

Williamson SC,Metcalf RL,Trapani F,Mohan S,Antonello J,Abbott B,Leong HS,Chester CP,Simms N,Polanski R,Nonaka D,Priest L,Fusi A,Carlsson F,Carlsson A,Hendrix MJ,Seftor RE,Seftor EA,Rothwell DG,Hughes A,Hicks J,Miller C,Kuhn P,Brady G,Simpson KL,Blackhall FH,Dive C

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunofluorescence

Wed Nov 09 00:00:00 UTC 2016

Vasculogenic mimicry in small cell lung cancer.

Nature communications

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunofluorescence

Wed Nov 09 00:00:00 UTC 2016

Direct conversion of human amniotic cells into endothelial cells without transitioning through a pluripotent state.

Nature protocols

Ginsberg M,Schachterle W,Shido K,Rafii S

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Tue Dec 01 00:00:00 UTC 2015

Direct conversion of human amniotic cells into endothelial cells without transitioning through a pluripotent state.

Nature protocols

Ginsberg M,Schachterle W,Shido K,Rafii S

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Tue Dec 01 00:00:00 UTC 2015

Mesenchymal stromal cells form vascular tubes when placed in fibrin sealant and accelerate wound healing in vivo.

Biomaterials

Mendez JJ,Ghaedi M,Sivarapatna A,Dimitrievska S,Shao Z,Osuji CO,Steinbacher DM,Leffell DJ,Niklason LE

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Sun Feb 01 00:00:00 UTC 2015

Histone Demethylases KDM4A and KDM4C Regulate Differentiation of Embryonic Stem Cells to Endothelial Cells.

Stem cell reports

Wu L,Wary KK,Revskoy S,Gao X,Tsang K,Komarova YA,Rehman J,Malik AB

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Tue Jul 14 00:00:00 UTC 2015

生物信息学

蛋白别名: 7B4 antigen; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; Vascular endothelial cadherin; VE-cadherin

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基因别名: 7B4; CD144; CDH5

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UniProt ID: (Human) P33151

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Entrez Gene ID: (Human) 1003

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功能

receptor binding calcium ion binding protein binding beta-catenin binding protein phosphatase binding ion channel binding cadherin

参与通路

blood vessel maturation cell-cell junction assembly homophilic cell adhesion via plasma membrane adhesion molecules transforming growth factor beta receptor signaling pathway negative regulation of cell proliferation single organismal cell-cell adhesion adherens junction organization positive regulation of establishment of endothelial barrier regulation of establishment of cell polarity