Invitrogen

CD158a/h/g Monoclonal Antibody (HP-MA4), PerCP-Cyanine5.5, eBioscience™

CD158a/h/g Antibody in Flow Cytometry (Flow)

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CD158a/h/g Antibody (45-1589-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42) and Mouse IgG2b K Isotype Control PerCP-Cyanine5-5 (Product # 45-4732-82) (left) or Anti-Human CD158a/h/g PerCP-Cyanine5-5 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

45-1589-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看2篇已发表文章 2篇已发表文章

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

HP-MA4

偶联物

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

激发/发射光谱

489/679 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1311133

产品详细信息

Description: The monoclonal antibody HP-MA4 recognizes several CD158 proteins, also known as KIR2D, specifically KIR2DL1 (CD158a), KIR2DS1 (CD158h), KIR2DS3, and KIR2DS5 (CD158g). It does not recognize KIR2DL2 (CD158b1), KIR2DS2 (CD158j), KIR2DL4 (CD158d) or KIR2DS4 (CD158i) and crossreactivity to KIR2DL3 (CD158b2) and KIR2DL5 (CD158f) are unknown. CD158 proteins are members of the killer cell immunoglobulin-like receptors (KIR) family of transmembrane glycoproteins that are expressed on NK cells and a subset of T cells. Each KIR has an isoform with either a short (S) or long (L) cytoplasmic domain which transduces either an activating or inhibitory signal, respectively. The crossreactivity of the HP-MA4 antibody to KIR2DS3 and KIR2DS5 was published in 2013.

Applications Reported: This HP-MA4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HP-MA4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes. The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

A Conserved HIV-1-Derived Peptide Presented by HLA-E Renders Infected T-cells Highly Susceptible to Attack by NKG2A/CD94-Bearing Natural Killer Cells.

PLoS pathogens

Davis ZB,Cogswell A,Scott H,Mertsching A,Boucau J,Wambua D,Le Gall S,Planelles V,Campbell KS,Barker E

45-1589 was used in Flow cytometry/Cell sorting to provide a mechanistic basis to guide these future clinical studies, suggesting that ex vivo-expanded NKG2A/CD94(+) KIR2DL(-) NK cells may be uniquely beneficial.

Mon Feb 01 00:00:00 UTC 2016

Expansion and homing of adoptively transferred human natural killer cells in immunodeficient mice varies with product preparation and in vivo cytokine administration: implications for clinical therapy.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Miller JS,Rooney CM,Curtsinger J,McElmurry R,McCullar V,Verneris MR,Lapteva N,McKenna D,Wagner JE,Blazar BR,Tolar J

45-1589 was used in Flow cytometry/Cell sorting to study whether natural killer cell product manipulations can optimise the kinetics and homing of natural killer cells in vivo.

Fri Aug 01 00:00:00 UTC 2014

A comprehensive analysis of the binding of anti-KIR antibodies to activating KIRs.

Genes and immunity

Czaja K,Borer AS,Schmied L,Terszowski G,Stern M,Gonzalez A

Wed Jan 01 00:00:00 UTC 2014

生物信息学

蛋白别名: CD158; CD158 antigen-like family member A; CD158 antigen-like family member G; CD158 antigen-like family member H; CD158 antigen-like family member I; CD158 antigen-like family member J; CD158a; CD158B1; CD158g; CD158h; CD158i; CD158j; Killer cell immunoglobulin-like receptor 2DL1; Killer cell immunoglobulin-like receptor 2DS1; Killer cell immunoglobulin-like receptor 2DS2; Killer cell immunoglobulin-like receptor 2DS4; Killer cell immunoglobulin-like receptor 2DS5; killer cell immunoglobulin-like receptor KIR2DS2; killer cell immunoglobulin-like receptor KIRDS1; killer cell immunoglobulin-like receptor two domains short cytoplasmic tail 1; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 1; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 2; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 5; killer Ig receptor; killer inhibitory receptor 2-2-1; killer inhibitory receptor 4-1-2; killer-cell Ig-like receptor; killer-cell immunoglobulin-like receptor; killer-cell immunoglobulin-like receptor two domains short tail 2 protein; KIR antigen 2DS4; MHC class I NK cell receptor; MHC class I NK cell receptor Eb6 ActI; natural killer associated transcript 5; natural killer cell inhibitory receptor; natural killer cell receptor; Natural killer-associated transcript 1; Natural killer-associated transcript 5; Natural killer-associated transcript 8; Natural killer-associated transcript 9; NK receptor 183 ActI; NKAT-1; NKAT-5; NKAT-8; NKAT-9; NKAT6; p50 killer cell activating receptor KAR-K1d; p50.3; p58 killer cell inhibitory receptor KIR-K64; p58 killer cell inhibitory receptor KIR-K7a; p58 natural killer cell receptor clone CL-49; P58 natural killer cell receptor clones CL-39/CL-17; p58 natural killer cell receptor clones CL-42/47.11; p58 NK cell inhibitory receptor NKR-K6; p58 NK receptor CL-39/CL-17; p58 NK receptor CL-42/47.11; p58 NK receptor CL-49; p58.1 MHC class-I-specific NK receptor; XXbac-BCX195L8.1

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基因别名: 183ActI; CD158A; CD158b; CD158G; CD158H; CD158I; CD158J; cl-49; KIR-2DS2; KIR-2DS4; KIR-K64; KIR1D; KIR221; KIR2DL1; KIR2DP1DL1; KIR2DS1; KIR2DS2; KIR2DS4; KIR2DS5; KIR412; KKA3; NKAT; NKAT-1; NKAT-5; NKAT-8; NKAT1; NKAT5; NKAT8; NKAT9; p50.1; p58.1