Invitrogen

CD16 Monoclonal Antibody (B73.1), PE, eBioscience™

1 Published Figure

9 References

View all (172) CD16 antibodies

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Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

FIGURE: 1 / 2

CD16 Antibody (12-0167-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) APC and Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD16 PE (right). Cells in the granulocyte gate were used for analysis.

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Product Details

12-0167-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B73.1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1603208

Product Specific Information

Description: This B73.1 monoclonal antibody reacts with human CD16, a member of the Fc receptor family also known as Fc gamma RIIIa and Fc gamma RIIIb. This 50-65 kDa receptor is expressed on natural killer cells, neutrophils, monocytes, and macrophages. Crosslinking of CD16 in natural killer cells and monocytes results in activation of the MAPK and PI3K signaling pathways, thus regulating the production of TNF alpha, IL-6, and IL-1 beta.

This monoclonal antibody has been shown to block Fc receptor functions.

Applications Reported: This B73.1 (B73.1.1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This B73.1 (B73.1.1) antibody has been pre-titrated and tested by flow cytometric analysis of normal peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Bone Marrow Mesenchymal Stromal Cell Treatment in Patients with Osteoarthritis Results in Overall Improvement in Pain and Symptoms and Reduces Synovial Inflammation.

Stem cells translational medicine

Chahal J,Gómez-Aristizábal A,Shestopaloff K,Bhatt S,Chaboureau A,Fazio A,Chisholm J,Weston A,Chiovitti J,Keating A,Kapoor M,Ogilvie-Harris DJ,Syed KA,Gandhi R,Mahomed NN,Marshall KW,Sussman MS,Naraghi AM,Viswanathan S

12-0167 was used in Flow cytometry/Cell sorting to provide important insights into BM-MSC dosing and BM-MSC reduction of synovial inflammation and cartilage degradation and provide a highly predictive donor selection criterion that will be critical in translating MSC therapy for osteoarthritis.

Thu Aug 01 00:00:00 UTC 2019

DKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation.

Nature medicine

Xiao Q,Wu J,Wang WJ,Chen S,Zheng Y,Yu X,Meeth K,Sahraei M,Bothwell ALM,Chen L,Bosenberg M,Chen J,Sexl V,Sun L,Li L,Tang W,Wu D

12-0167 was used in Flow cytometry/Cell sorting to identify a novel tumour immune-suppressive mechanism and immunotherapeutic targets particularly relevant for colorectal cancers and a subset of melanomas.

Thu Mar 01 00:00:00 UTC 2018

Mechanical strain determines the site-specific localization of inflammation and tissue damage in arthritis.

Nature communications

Cambré I,Gaublomme D,Burssens A,Jacques P,Schryvers N,De Muynck A,Meuris L,Lambrecht S,Carter S,de Bleser P,Saeys Y,Van Hoorebeke L,Kollias G,Mack M,Simoens P,Lories R,Callewaert N,Schett G,Elewaut D

12-0167 was used in Flow cytometry/Cell sorting to study the role of mechanical strain in controlling site-specific localisation of inflammation and tissue damage in arthritis.

Mon Nov 05 00:00:00 UTC 2018

The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence.

Cell death & disease

Ong SM,Hadadi E,Dang TM,Yeap WH,Tan CT,Ng TP,Larbi A,Wong SC

Thu Feb 15 00:00:00 UTC 2018

Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system.

Journal of neurovirology

Garcia-Mesa Y,Jay TR,Checkley MA,Luttge B,Dobrowolski C,Valadkhan S,Landreth GE,Karn J,Alvarez-Carbonell D

12-0167 was used in Flow cytometry/Cell sorting to show how the immortalization of primary microglia can provide a platform upon which to study HIV regulation in the central nervous system.

Wed Feb 01 00:00:00 UTC 2017

Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system.

Journal of neurovirology

Garcia-Mesa Y,Jay TR,Checkley MA,Luttge B,Dobrowolski C,Valadkhan S,Landreth GE,Karn J,Alvarez-Carbonell D

12-0167 was used in Flow cytometry/Cell sorting to show how the immortalization of primary microglia can provide a platform upon which to study HIV regulation in the central nervous system.

Wed Feb 01 00:00:00 UTC 2017

Cytoplasmic Localization of HTLV-1 HBZ Protein: A Biomarker of HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP).

PLoS neglected tropical diseases

Baratella M,Forlani G,Raval GU,Tedeschi A,Gout O,Gessain A,Tosi G,Accolla RS

12-0167 was used in Flow cytometry/Cell sorting to identify the cytoplasmic localization of HTLV-1 HBZ protein as a possible biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis.

Sun Jan 01 00:00:00 UTC 2017

The Phenotypic Characterization of the Human Renal Mononuclear Phagocytes Reveal a Co-Ordinated Response to Injury.

PloS one

Leone DA,Kozakowski N,Kornauth C,Waidacher T,Neudert B,Loeffler AG,Haitel A,Rees AJ,Kain R

12-0167 was used in Flow cytometry/Cell sorting to analyse the characteristics and constitution of mononuclear phagocyte populations in human kidneys.

Tue Aug 09 00:00:00 UTC 2016

Phosphoantigen/IL2 expansion and differentiation of Vγ2Vδ2 T cells increase resistance to tuberculosis in nonhuman primates.

PLoS pathogens

Chen CY,Yao S,Huang D,Wei H,Sicard H,Zeng G,Jomaa H,Larsen MH,Jacobs WR,Wang R,Letvin N,Shen Y,Qiu L,Shen L,Chen ZW

12-0167 was used in Flow cytometry/Cell sorting to assess the potential of γδ T-cell-targeted treatment of established TB.

Thu Aug 01 00:00:00 UTC 2013

Monocytes regulate the mechanism of T-cell death by inducing Fas-mediated apoptosis during bacterial infection.

PLoS pathogens

Daigneault M,De Silva TI,Bewley MA,Preston JA,Marriott HM,Mitchell AM,Mitchell TJ,Read RC,Whyte MK,Dockrell DH

12-0167 was used in Flow cytometry/Cell sorting to elucidate the mechanisms that regulate levels of activated T-cells at sites of acute bacterial infection.

Tue Jan 29 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: CD16-I; CD16-II; CD16a; CD16a antigen; CD16b; CD32; CD32 receptor 2; CDw32; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III-A; Fc gamma receptor IIIa; Fc gamma receptor IIIb; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; Fc-gamma RIIIa; fc-gamma RIIIb; Fc-gamma-RIIb; Fc-gamma-RIIc; FCG2; FcgammaRIIIA; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; IgG Fc receptor III-1; IgG Fc receptor III-2; IgG Fc receptor III-A; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; neutrophil-specific antigen NA; RP11-5K23.1

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Gene Aliases: CD16; CD16A; CD16B; FCG3; FCGR3; FCGR3A; FCGR3B; FCGRIII; FCR-10; FCRIII; FCRIIIA; FCRIIIb; IGFR3; IMD20

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UniProt ID: (Human) P08637, (Human) O75015

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Entrez Gene ID: (Human) 2214, (Human) 2215

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Function(s)

IgG binding immunoglobulin receptor superfamily

Process(es)

immune response Fc-gamma receptor signaling pathway involved in phagocytosis regulation of immune response positive regulation of defense response to virus by host mitophagy in response to mitochondrial depolarization xenophagy

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CD16 Monoclonal Antibody (B73.1), PE, eBioscience™

CD16 Antibody (12-0167-42) in Flow

Product Details

12-0167-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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