Invitrogen

CD163 Monoclonal Antibody (TNKUPJ), eBioscience™

CD163 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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CD163 Antibody (14-1631-82) in IHC (F)

Immunohistochemistry of frozen mouse spleen stained with 5 µg/mL of Rat IgG2a kappa Isotype Control (Product # 14-4321-82) (left) or 5 µg/mL of Anti-Mouse CD163 (right) followed by Anti-Rat IgG TRITC (Product # 26-4826-82). Nuclei are stained with DAPI.

CD163 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

CD163 Antibody in Immunohistochemistry (IHC)

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产品信息

14-1631-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

5 µg/mL

流式细胞分析 (Flow)

查看11篇已发表文章 11篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

TNKUPJ

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_2716934

产品详细信息

Description: This TNKUPJ monoclonal antibody recognizes mouse CD163. CD163 is a 130 kDa surface receptor expressed by certain subsets of tissue macrophages, including splenic red pulp macrophages, Kupffer cells, intestinal lamina propria macrophages and a small fraction of peritoneal macrophages. In contrast to human blood monocytes, mouse monocytes do not express CD163. Also, unlike human CD163, mouse CD163 is not as readily induced by M2 polarizing cytokines, and it is not a good marker of M2 macrophages. No common cell lines of monocytic or macrophage origin express mouse CD163. In humans, CD163 has been shown to be proteolytically cleaved and shed from the cell surface, and it acts as a soluble anti-inflammatory factor.

This TNKUPJ antibody will detect CD163 on fixed and permeabilized cells allowing for staining of the intracellular pool of this receptor. Although CD163 is relatively stable to collagenase digestion, aggressive tissue dissociation protocols might potentially decrease the amount of surface CD163. In these cases intracellular detection is recommended.

Applications Reported: This TNKUPJ antibody has been reported for use in immunohistochemical staining of frozen tissue sections. Fluorochrome-conjugated TNKUPJ is recommended for use in flow cytometry.

Applications Tested: This TNKUPJ antibody has been tested by immunohistochemical staining of frozen tissue sections. This may be used at less than or equal to 5.0 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

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Cellular and molecular gastroenterology and hepatology

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Pericyte stem cells induce Ly6G⁺ cell accumulation and immunotherapy resistance in pancreatic cancer.

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Oral Delivery of Transformable Bilirubin Self-Assembled System for Targeted Therapy of Colitis.

Advanced healthcare materials

Chen Z,Chen Y,Hao W,Shui M,Zhang J,Zhou H,Zhang C,Wang Y,Wang S

14-1631-82 was used in Immunohistochemistry to provide a colon-targeted and transformable BSNM that is simple to prepare and is useful as an efficient targeted UC therapy.

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An enriched environment re-establishes metabolic homeostasis by reducing obesity-induced inflammation.

Disease models & mechanisms

Díaz de León-Guerrero S,Salazar-León J,Meza-Sosa KF,Valle-Garcia D,Aguilar-León D,Pedraza-Alva G,Pérez-Martínez L

14-1631-82 was used in Immunohistochemistry to explore whether an enriched environment is capable of restoring energy balance in obese mice that previously presented metabolic alterations.

Wed Jun 01 00:00:00 UTC 2022

Exosomal AP000439.2 from clear cell renal cell carcinoma induces M2 macrophage polarization to promote tumor progression through activation of STAT3.

Cell communication and signaling : CCS

Shen T,Miao S,Zhou Y,Yi X,Xue S,Du B,Tang C,Qu L,Fu D,Jia R,He H

Published figure using CD163 monoclonal antibody (Product # 14-1631-82) in Flow Cytometry

Sat Sep 24 00:00:00 UTC 2022

Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy.

Cell reports

Wang W,Wu S,Cen Z,Zhang Y,Chen Y,Huang Y,Cillo AR,Prokopec JS,Quarato G,Vignali DAA,Stewart-Ornstein J,Li S,Lu B,Gong YN

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Restoring Prohealing/Remodeling-Associated M2a/c Macrophages Using ON101 Accelerates Diabetic Wound Healing.

JID innovations : skin science from molecules to population health

Lin CW,Chen CC,Huang WY,Chen YY,Chen ST,Chou HW,Hung CM,Chen WJ,Lu CS,Nian SX,Chen SG,Chang HW,Chang VHS,Liu LY,Kuo ML,Chang SC

Published figure using CD163 monoclonal antibody (Product # 14-1631-82) in Flow Cytometry

Thu Sep 01 00:00:00 UTC 2022

The regional distribution of resident immune cells shapes distinct immunological environments along the murine epididymis.

eLife

Pleuger C,Ai D,Hoppe ML,Winter LT,Bohnert D,Karl D,Guenther S,Epelman S,Kantores C,Fijak M,Ravens S,Middendorff R,Mayer JU,Loveland KL,Hedger M,Bhushan S,Meinhardt A

Published figure using CD163 monoclonal antibody (Product # 14-1631-82) in Immunohistochemistry

Wed Dec 14 00:00:00 UTC 2022

Tumor cell-derived asymmetric dimethylarginine regulates macrophage functions and polarization.

Cancer cell international

Chen YL,Lowery AT,Lin S,Walker AM,Chen KE

Published figure using CD163 monoclonal antibody (Product # 14-1631-82) in Flow Cytometry

Tue Nov 15 00:00:00 UTC 2022

Tumour-associated macrophages drive stromal cell-dependent collagen crosslinking and stiffening to promote breast cancer aggression.

Nature materials

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14-1631-82 was used in Immunohistochemistry (Paraffin) to link tissue inflammation, stromal cell-mediated collagen crosslinking and stiffening to tumour aggression and identify lysyl hydroxylase 2 as a stromal biomarker.

Thu Apr 01 00:00:00 UTC 2021

Tumour-associated macrophages drive stromal cell-dependent collagen crosslinking and stiffening to promote breast cancer aggression.

Nature materials

Thu Apr 01 00:00:00 UTC 2021

Tumour-associated macrophages drive stromal cell-dependent collagen crosslinking and stiffening to promote breast cancer aggression.

Nature materials

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Exosomes derived from inflammatory myoblasts promote M1 polarization and break the balance of myoblast proliferation/differentiation.

World journal of stem cells

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Pulmonary transplantation of alpha-1 antitrypsin (AAT)-transgenic macrophages provides a source of functional human AAT in vivo.

Gene therapy

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Published figure using CD163 monoclonal antibody (Product # 14-1631-82) in Flow Cytometry

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Magnoliae Cortex Alleviates Muscle Wasting by Modulating M2 Macrophages in a Cisplatin-Induced Sarcopenia Mouse Model.

International journal of molecular sciences

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Convallatoxin Promotes M2 Macrophage Polarization to Attenuate Atherosclerosis Through PPARγ-Integrin α_vβ₅ Signaling Pathway.

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CCL2 and CXCL12 Derived from Mesenchymal Stromal Cells Cooperatively Polarize IL-10+ Tissue Macrophages to Mitigate Gut Injury.

Cell reports

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CD163+ macrophages promote angiogenesis and vascular permeability accompanied by inflammation in atherosclerosis.

The Journal of clinical investigation

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