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-Flow-20170216130641.jpg?time=20240412 "CD166 (ALCAM) Antibody in Flow Cytometry (Flow)")
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CD166 (ALCAM) Antibody (14-1661-82) in Flow
Staining of C57BL/6 splenocytes with 0.125 µg of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.125 µg of Anti-Mouse CD166 (ALCAM) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
-Flow-20170216130641.jpg?time=20240412)
-Flow-20170216130641.jpg?time=20240412?time=20240412 "CD166 (ALCAM) Antibody (14-1661-82) in Flow")
产品信息
14-1661-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBioALC48
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_823127
产品详细信息
Description: Activated leukocyte cell adhesion molecule (ALCAM; CD166) is a 105 kD cell-surface adhesion molecule belonging to the Ig superfamily of receptors. ALCAM is a type I transmembrane glycoprotein, possessing five extracellular Ig domains, a transmembrane domain, and a short cytoplasmic tail. ALCAM is involved in homotypic adhesion, as well as heterotypic adhesion with its natural ligand, CD6. A soluble isoform of ALCAM has been shown to inhibit homophilic adhesive interactions. ALCAM is expressed on a variety of cell types, including activated T and B cells, monocyte-derived dendritic cells, and thymic epithelial cells. ALCAM expression has also been used as a marker for the isolation of pluripotent stem cells.
Applications Reported: This eBioALC-48 antibody has been reported for use in flow cytometric analysis and immunohistochemistry on paraffin-embedded tissue sections.
Applications Tested: The eBioALC-48 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Activated leukocyte cell adhesion molecule; BEN; CD166; CD166 antigen; FLJ38514; MGC71733; Protein DM-GRASP
基因别名: AI853494; Alcam; BEN; CD166; DM-GRASP; MuSC; SC1
UniProt ID: (Mouse) Q61490
Entrez Gene ID: (Mouse) 11658
