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产品信息
14-1779-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
MEM-166 (MEM166)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_657647
产品详细信息
Description: The MEM-166 monoclonal antibody reacts with human CD177, a 58-64 kDa GPI-anchored glycoprotein. The human CD177 locus codes for at least two alleles, NB1 and PRV-1, which differ by only 4 amino acids. CD177 is expressed on granulocytes, early erythroblasts, megakaryocytes, promyelocytes and myelocytes. CD177 mRNA levels are elevated in several different myeloproliferative disorders including polycythemia vera, and antibodies to CD177 are involved in neonatal alloimmune neutropenia, autoimmune drug inducedneutropenia, and graft failure following marrow transplantation.
Applications Reported: This MEM-166 (MEM166) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB) (non-reducing conditions).
Applications Tested: This MEM-166 (MEM166) antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD177; CD177 antigen; cell surface receptor; HNA-2a; Human neutrophil alloantigen 2a; NB1 glycoprotein; NB1 GP; Polycythemia rubra vera protein 1; PRV-1
基因别名: CD177; HNA-2a; HNA2A; NB1; NB1 GP; PRV-1; PRV1; UNQ595/PRO1181
UniProt ID: (Human) Q8N6Q3
Entrez Gene ID: (Human) 57126
