Invitrogen

CD18 Monoclonal Antibody (TA-4)

CD18 Antibody in Immunocytochemistry (ICC/IF)

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CD18 Antibody (MA1807) in ICC/IF

Immunofluorescence analysis of CD18 Monoclonal Antibody (TA-4) was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD18 (TA-4) Mouse Monoclonal Antibody (Product # MA1807) at 2 µg/mL in 0.1% BSA, incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 con... View More

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产品信息

MA1807

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

Assay-dependent

酶联免疫吸附实验 (ELISA)

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中和实验 (Neu)

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产品规格

种属反应

Human, Rat

已发表种属

Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

TA-4

抗原

Rat beta2 Integrin

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_223589

产品详细信息

MA1807 targets CD18 in FACS applications and shows reactivity with Rat samples.

The MA1807 immunogen is rat beta2 Integrin.

MA1807 detects CD18 which has a predicted molecular weight of approximately 83 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

靶标信息

CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes. CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling. CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the CD18 protein have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

VCAM-1 has a tissue-specific role in mediating interleukin-4-induced eosinophil accumulation in rat models: evidence for a dissociation between endothelial-cell VCAM-1 expression and a functional role in eosinophil migration.

Blood

Larbi KY,Allen AR,Tam FW,Haskard DO,Lobb RR,Silva PM,Nourshargh S

MA1807 was used in ELISA to investigate the role of endothelial-cell VCAM-1 in eosinophil accumulation

Wed Nov 15 00:00:00 UTC 2000

Intratracheal injection of endotoxin and cytokines. IX. Contribution of CD11a/ICAM-1 to neutrophil emigration.

The American journal of physiology

Tang WW,Yi ES,Remick DG,Wittwer A,Yin S,Qi M,Ulich TR

MA1807 was used in blocking/activating experiment to investigate the mechanism of neutrophil emigration induced by lipopolysaccharide and tumor necrosis factor

Wed Nov 01 00:00:00 UTC 1995

生物信息学

蛋白别名: CD18; CD18 leukocyte adhesion molecule; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; Complement receptor C3 subunit beta; integrin beta chain, beta 2; Integrin beta-2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; macrophage antigen 1 (mac-1) beta subunit)

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基因别名: CD18; ITGB2; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7

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UniProt ID: (Human) P05107

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Entrez Gene ID: (Human) 3689, (Rat) 309684

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功能

glycoprotein binding protein binding protein kinase binding ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding

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