Invitrogen

CD19 Monoclonal Antibody (LC1), eBioscience™

CD19 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD19 Antibody (14-0190-82) in IHC (P)

Immunohistochemical analysis of CD19 was performed using formalin-fixed paraffin-embedded mouse thymus and mouse brain tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for ... View More

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CD19 Antibody — Antibody specificity was demonstrated by the relative expression of target protein in different tissues by IHC. Differential expression of CD19 in mouse thymus and mouse brain tissues using CD19 Monoclonal Antibody (LC1), eBioscience™ (Product # 14-0190-82) shows strong staining of CD19 in mouse thymus tissue and no staining in mouse brain tissue. {RE}

产品信息

14-0190-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-5 µg/mL

免疫组化（石蜡） (IHC (P))

1-10 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

LC1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_11219274

产品详细信息

Description: The LC1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multi-molecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The antibody recognizes a sequence near the carboxy terminus.

Applications Reported: This LC1 antibody has been reported for use in western blotting and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This LC1 antibody has been tested by western blot and can be used at less than or equal to 5 µg/mL. This antibody has also been tested by immunohistochemistry on FFPE human tonsil using high pH antigen retrieval at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。