Invitrogen

CD19 Monoclonal Antibody (SJ25C1), PE-Cyanine7, eBioscience™

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CD19 Antibody (25-0198-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0037-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human CD19 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD19 Antibody — Antibody clone (SJ25C1) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in CD19 KO cells (blue histogram) compared to the control Cas9 cells (pink histogram) using CD19 antibody (SJ25C1). Yellow histogram represents staining with the isotype control. {KO}

产品信息

25-0198-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看16篇已发表文章 16篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

SJ25C1

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10671548

产品详细信息

Description: The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express this molecule. CD19, along with CD21, CD81, Leu13, and MHC class II, form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.

Applications Reported: This SJ25C1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SJ25C1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Tyrosine Kinase Inhibitors Target B Lymphocytes.

Biomolecules

Upfold NLE,Petakh P,Kamyshnyi A,Oksenych V

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Sat Feb 25 00:00:00 UTC 2023

Evaluation of peripheral lymphocyte subsets in acne vulgaris patients before and after systemic isotretinoin treatment.

Indian journal of pharmacology

Gunaydin SD,Tezcan I

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Wed Dec 21 00:00:00 UTC 2022

Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.

Cancer discovery

Du W,Hua F,Li X,Zhang J,Li S,Wang W,Zhou J,Wang W,Liao P,Yan Y,Li G,Wei S,Grove S,Vatan L,Zgodziński W,Majewski M,Wallner G,Chen H,Kryczek I,Fang JY,Zou W

25-0198-42 was used in Flow Cytometry to show that optineurin is a shared node between the IFN and MHC pathways and predicts colorectal cancer patient outcome.

Thu Jul 01 00:00:00 UTC 2021

Innate-like self-reactive B cells infiltrate human renal allografts during transplant rejection.

Nature communications

Asano Y,Daccache J,Jain D,Ko K,Kinloch A,Veselits M,Wolfgeher D,Chang A,Josephson M,Cunningham P,Tambur A,Khan AA,Pillai S,Chong AS,Clark MR

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Fri Jul 16 00:00:00 UTC 2021

Human chimeric antigen receptor macrophages for cancer immunotherapy.

Nature biotechnology

Klichinsky M,Ruella M,Shestova O,Lu XM,Best A,Zeeman M,Schmierer M,Gabrusiewicz K,Anderson NR,Petty NE,Cummins KD,Shen F,Shan X,Veliz K,Blouch K,Yashiro-Ohtani Y,Kenderian SS,Kim MY,O'Connor RS,Wallace SR,Kozlowski MS,Marchione DM,Shestov M,Garcia BA,June CH,Gill S

25-0198-42 was used in Flow Cytometry to show that in humanized mouse models, CAR-Ms were further shown to induce a pro-inflammatory tumor microenvironment and boost anti-tumor T cell activity.

Sat Aug 01 00:00:00 UTC 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

25-0198 was used in Flow cytometry/Cell sorting to investigate if downregulated membrane expression of CD18 characterises a primitive population of human hematopoietic repopulating cells.

Tue Apr 28 00:00:00 UTC 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

25-0198 was used in Flow cytometry/Cell sorting to investigate if downregulated membrane expression of CD18 characterises a primitive population of human hematopoietic repopulating cells.

Tue Apr 28 00:00:00 UTC 2020

Human C. difficile toxin-specific memory B cell repertoires encode poorly neutralizing antibodies.

JCI insight

Shah HB,Smith K,Scott EJ,Larabee JL,James JA,Ballard JD,Lang ML

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Thu Aug 20 00:00:00 UTC 2020

Identifying the Pathological Domain of Alpha- Synuclein as a Therapeutic for Parkinson's Disease.

International journal of molecular sciences

Shen N,Song G,Yang H,Lin X,Brown B,Hong Y,Cai J,Cao C

25-0198-42 was used in Flow Cytometry to identify the most potent therapeutic domain of alpha synuclein as a therapeutic target.

Sat May 11 00:00:00 UTC 2019

Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.

The Journal of experimental medicine

Carpier JM,Zucchetti AE,Bataille L,Dogniaux S,Shafaq-Zadah M,Bardin S,Lucchino M,Maurin M,Joannas LD,Magalhaes JG,Johannes L,Galli T,Goud B,Hivroz C

25-0198 was used in Flow cytometry/Cell sorting to study the retrograde traffic of linker for activation of T cells from the plasma membrane to the Golgi-trans-Golgi network.

Mon Apr 02 00:00:00 UTC 2018

Efficient Non-viral Gene Delivery into Human Hematopoietic Stem Cells by Minicircle Sleeping Beauty Transposon Vectors.

Molecular therapy : the journal of the American Society of Gene Therapy

Holstein M,Mesa-Nuñez C,Miskey C,Almarza E,Poletti V,Schmeer M,Grueso E,Ordóñez Flores JC,Kobelt D,Walther W,Aneja MK,Geiger J,Bonig HB,Izsvák Z,Schleef M,Rudolph C,Mavilio F,Bueren JA,Guenechea G,Ivics Z

25-0198 was used in Flow cytometry/Cell sorting to improve Sleeping Beauty gene transfer into hard-to-transfect human CD34+ cells.

Wed Apr 04 00:00:00 UTC 2018

Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation.

Journal of immunology (Baltimore, Md. : 1950)

Dai J,Luftig MA

25-0198-42 was used in Flow Cytometry to find that naive and memory B cells were BCL-2-dependent, whereas germinal center B cells were MCL-1-dependent and plasma cells were BCL-XL-dependent.

Thu Mar 01 00:00:00 UTC 2018

Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation.

Journal of immunology (Baltimore, Md. : 1950)

Dai J,Luftig MA

25-0198-42 was used in Flow Cytometry to find that naive and memory B cells were BCL-2-dependent, whereas germinal center B cells were MCL-1-dependent and plasma cells were BCL-XL-dependent.

Thu Mar 01 00:00:00 UTC 2018

ILC2-modulated T cell-to-MDSC balance is associated with bladder cancer recurrence.

The Journal of clinical investigation

Chevalier MF,Trabanelli S,Racle J,Salomé B,Cesson V,Gharbi D,Bohner P,Domingos-Pereira S,Dartiguenave F,Fritschi AS,Speiser DE,Rentsch CA,Gfeller D,Jichlinski P,Nardelli-Haefliger D,Jandus C,Derré L

25-0198 was used in Flow cytometry/Cell sorting to underline the ILC2/IL-13 axis as a targetable pathway to curtail the M-MDSC compartment and improve bladder cancer treatment.

Tue Aug 01 00:00:00 UTC 2017

Antibody-Dependent NK Cell Activation Differentially Targets EBV-Infected Cells in Lytic Cycle and Bystander B Lymphocytes Bound to Viral Antigen-Containing Particles.

Journal of immunology (Baltimore, Md. : 1950)

López-Montañés M,Alari-Pahissa E,Sintes J,Martínez-Rodríguez JE,Muntasell A,López-Botet M

25-0198 was used in Flow cytometry/Cell sorting to support that Ab-dependent NK cell activation plays an important role in the control of EBV, enhancing NK cell effector functions against infected B cells in the lytic cycle.