Invitrogen

CD195 (CCR5) Monoclonal Antibody (HM-CCR5 (7A4)), PerCP-eFluor™ 710, eBioscience™

CD195 (CCR5) Antibody in Flow Cytometry (Flow)

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CD195 (CCR5) Antibody (46-1951-82) in Flow

Staining of FcR-blocked (Anti-Mouse CD16/CD32 Purified, Product # 14-0161-82) BALB/c peritoneal exudate cells with Anti-Mouse CD11b FITC (Product # 11-0112-41) and 0.125 µg of Armenian Hamster IgG Isotype Control PerCP-eFluor® 710 (Product # 46-4888-82) (left) or 0.125 µg of Anti-Mouse CD195 (CCR5) PerCP-eFluor® 710 (right). Cells in the large scatter population were used for analysis.

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产品信息

46-1951-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看5篇已发表文章 5篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

HM-CCR5 (7A4)

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11149119

产品详细信息

Description: CCR5 is the major co-receptor for macrophage-tropic isolates of HIV-1. CCR5, also known as CD195, is a seven transmembrane chemokine receptor capable of binding RANTES, MIP-1 alpha and MIP-1 beta. Knockout mice show a decreased recruitment of macrophages and increased susceptibility to infection. Protein and mRNA has been found in macrophages from thioglycolate-treated and activated T cells.

The HM-CCR5(7A4) monoclonal antibody binds the N terminal extracellular domain of mouse CCR5 with no crossreactivity to human CCR5.

Applications Reported: This HM-CCR5 (7A4) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HM-CCR5 (7A4) antibody has been tested by flow cytometric analysis of mouse thioglycolated- induced peritoneal exudate cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The beta-chemokines RANTES, MIP-alpha and MIP-beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

BCL-xL inhibition potentiates cancer therapies by redirecting the outcome of p53 activation from senescence to apoptosis.

Cell reports

Bharti V,Watkins R,Kumar A,Shattuck-Brandt RL,Mossing A,Mittra A,Shen C,Tsung A,Davies AE,Hanel W,Reneau JC,Chung C,Sizemore GM,Richmond A,Weiss VL,Vilgelm AE

46-1951-82 was used in Flow Cytometry to show a crosstalk between the mitochondrial apoptotic pathway and cell cycle regulation that can be targeted to augment therapeutic efficacy in cancers with wild-type p53.

Tue Dec 20 00:00:00 UTC 2022

Role of nuclear localization in the regulation and function of T-bet and Eomes in exhausted CD8 T cells.

Cell reports

McLane LM,Ngiow SF,Chen Z,Attanasio J,Manne S,Ruthel G,Wu JE,Staupe RP,Xu W,Amaravadi RK,Xu X,Karakousis GC,Mitchell TC,Schuchter LM,Huang AC,Freedman BD,Betts MR,Wherry EJ

46-1951-82 was used in Flow cytometry-immunoflourescence to investigate if subcellular localization of T-bet and Eomes dictate their regulatory activity in exhausted T cells (TEXs).

Tue May 11 00:00:00 UTC 2021

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

Published figure using CD195 (CCR5) monoclonal antibody (Product # 46-1951-82) in Flow Cytometry

Tue Mar 05 00:00:00 UTC 2019

CD4⁺ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema.

Nature communications

García Nores GD,Ly CL,Cuzzone DA,Kataru RP,Hespe GE,Torrisi JS,Huang JJ,Gardenier JC,Savetsky IL,Nitti MD,Yu JZ,Rehal S,Mehrara BJ

Published figure using CD195 (CCR5) monoclonal antibody (Product # 46-1951-82) in Flow Cytometry

Thu May 17 00:00:00 UTC 2018

Mitogen-Activated Protein Kinase 2 Signaling Shapes Macrophage Plasticity in Aggregatibacter actinomycetemcomitans-Induced Bone Loss.

Infection and immunity

Herbert BA,Steinkamp HM,Gaestel M,Kirkwood KL

46-1951 was used in Flow cytometry/Cell sorting to suggest that MK2 in macrophages contributes to regulation of chemokine signaling during A. actinomycetemcomitans-induced inflammation and bone loss.