Invitrogen

CD196 (CCR6) Monoclonal Antibody (R6H1), eBioscience™

CD196 (CCR6) Antibody in Western Blot (WB)

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CD196 (CCR6) Antibody (14-1969-82) in WB

Immunoblotting of 10e6 normal peripheral blood cell lysates with 5 µg/mL of Anti-Human CD196 (CCR6) Purified. The band was visualized using Anti-Mouse IgG HRP.

CD196 (CCR6) Antibody in Flow Cytometry (Flow)

CD196 (CCR6) Antibody in Immunocytochemistry (ICC/IF)

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产品信息

14-1969-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

5 µg/mL

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

Assay-Dependent

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免疫沉淀 (IP)

Assay-Dependent

其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

R6H1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_1257199

产品详细信息

Description: This R6H1 monoclonal antibody reacts with CD196 (also known as CCR6), a seven transmembrane G protein-coupled receptor expressed on T, B, dendritic, natural killer, and Langerhans cells. This CC chemokine receptor uniquely binds MIP-3a/CCL20, a chemoattractant for dendritic cells, effector/memory T cells, and B cells. CD196 is also involved in host defense and inflammation at epithelial sites. Furthermore, this receptor has been implicated in Th17 differentiation and CD4+FoxP3+ regulatory T cell development.

Applications Reported: This R6H1 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunoprecipitation.

Applications Tested: This R6H1 antibody has been tested by western blot on lysates prepared from normal human peripheral blood cells. This antibody can be used at less than or equal to 5 µg/mL.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Common transcriptional programs and the role of chemokine (C-C motif) ligand 20 (CCL20) in cell migration of cholangiocarcinoma.

EXCLI journal

Win Maung HM,Chan-On W,Kunkeaw N,Khaenam P

14-1969-82 was used in Western Blot to analyze multiple microarray datasets to identify the common transcriptional networks in CCA and select a possible biomarker for functional study in CCA cell lines.

Mon Sep 28 00:00:00 UTC 2020

Common transcriptional programs and the role of chemokine (C-C motif) ligand 20 (CCL20) in cell migration of cholangiocarcinoma.

EXCLI journal

Win Maung HM,Chan-On W,Kunkeaw N,Khaenam P

Mon Sep 28 00:00:00 UTC 2020

Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.

Genes

Zou M,Zhang X,Jiang D,Zhao Y,Wu T,Gong Q,Su H,Wu D,Moreland L,Li G

Published figure using CD196 (CCR6) monoclonal antibody (Product # 14-1969-82) in Western Blot

Mon Dec 21 00:00:00 UTC 2020

The Calcineurin Inhibitor Tacrolimus Specifically Suppresses Human T Follicular Helper Cells.

Frontiers in immunology

Wallin EF,Hill DL,Linterman MA,Wood KJ

14-1969 was used in Flow cytometry/Cell sorting to study the impact of the immunosuppressive agent Tacrolimuson T follicular helper cells differentiationand function in transplant recipients.

Fri Aug 16 00:00:00 UTC 2019

The Rheumatoid Arthritis Risk Variant CCR6DNP Regulates CCR6 via PARP-1.

PLoS genetics

Li G,Cunin P,Wu D,Diogo D,Yang Y,Okada Y,Plenge RM,Nigrovic PA

14-1969 was used in Western Blotting to investigate whether CCR6DNP is causal for rheumatoid arthritis.

Thu Sep 01 00:00:00 UTC 2016

Expression of CCL20 and Its Corresponding Receptor CCR6 Is Enhanced in Active Inflammatory Bowel Disease, and TLR3 Mediates CCL20 Expression in Colonic Epithelial Cells.

PloS one

Skovdahl HK,Granlund Av,Østvik AE,Bruland T,Bakke I,Torp SH,Damås JK,Sandvik AK

Tue Jun 14 00:00:00 UTC 2016

CCR6 is a prognostic marker for overall survival in patients with colorectal cancer, and its overexpression enhances metastasis in vivo.

PloS one

Liu J,Ke F,Xu Z,Liu Z,Zhang L,Yan S,Wang Z,Wang H,Wang H

Published figure using CD196 (CCR6) monoclonal antibody (Product # 14-1969-82) in Western Blot

Thu Feb 12 00:00:00 UTC 2015

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD196 (CCR6) monoclonal antibody (Product # 14-1969-82) in Immunofluorescence

Fri Oct 13 00:00:00 UTC 2017

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD196 (CCR6) monoclonal antibody (Product # 14-1969-82) in Immunofluorescence

Fri Oct 13 00:00:00 UTC 2017

生物信息学

蛋白别名: C-C chemokine receptor type 6; C-C CKR-6; CD196; chemokine (C-C motif) receptor 6; chemokine (C-C) receptor 6; Chemokine receptor-like 3; CKR-L3; CKR6; DRY-6; DRY6; G protein-coupled receptor 29; G-protein coupled receptor 29; GPR-CY4; GPRCY4; LARC receptor; seven-transmembrane receptor, lymphocyte, 22

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基因别名: BN-1; C-C CKR-6; CC-CKR-6; CCR-6; CCR6; CD196; CKR-L3; CKRL3; CMKBR6; DCR2; DRY6; GPR29; GPRCY4; STRL22

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UniProt ID: (Human) P51684

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Entrez Gene ID: (Human) 1235

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功能

receptor activity chemokine receptor activity protein binding C-C chemokine receptor activity C-C chemokine binding

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