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CD201 (EPCR) Antibody (16-2011-81) in Flow
Staining of bEnd-3 cells with 0.125 µg of Rat IgG2a K Isotype Control Functional Grade Purified (Product # 16-4321-82) (blue histogram) or 0.125 µg of Anti-Mouse CD201 (EPCR) Functional Grade Purified (purple histogram) followed by Anti-Rat IgG PE (Product # 12-4822).Total viable cells were used for analysis.
产品信息
16-2011-81
产品规格
种属反应
Mouse
已发表种属
Not Applicable
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBiomRCR-16 (mRCR-16)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1724023
产品详细信息
Description: The eBioRCR-16 monoclonal antibody reacts with mouse Endothelial Protein C Receptor (EPCR, CD201), a 25 kDa Type 1 transmembrane protein expressed on endothelial cells. EPCR exhibits sequence and structural homology with the MHC class I/CD1 family of proteins. EPCR is a ligand for Protein C and plays an important role in augmenting Protein C activation by the thrombin-thrombomodulin complex and in regulating blood coagulation and inflammation. Deletion of EPCR results in embryonic lethality, at least partially due to placental thrombosis.
Recently, it was demonstrated that EPCR expression identified cells in the bone marrow that are capable of hematopoietic reconstitution activity comparable to hematopoietic stem cells isolated with conventional methods. The eBio1560 monoclonal antibody can be used for the detection of these hematopoietic stem cells, however the eBiomRCR-16 monoclonal antibody should only be used for the detection of CD201 on endothelial cells.
Applications Reported: This eBiomRCR-16 (mRCR-16) antibody has been reported for use in flow cytometric analysis and in functional assays.
Applications Tested: This eBiomRCR-16 (mRCR-16) antibody has been tested by flow cytometric analyis of the bEnd.3 cell line. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Activated protein C receptor; APC receptor; bA42O4.2; CD antigen CD201; CD201; Centrocyclin; Centrosomal protein CCD41; Endothelial cell protein C receptor; Endothelial protein C receptor
基因别名: AI325044; Ccca; Ccd41; Epcr; Procr
UniProt ID: (Mouse) Q64695
Entrez Gene ID: (Mouse) 19124
