Invitrogen

CD202b (TIE2) Monoclonal Antibody (33.1), eBioscience™

CD202b (TIE2) Antibody in Western Blot (WB)

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CD202b (TIE2) Antibody (14-2029-82) in WB

Western blot was performed using CD202b (TIE2) Monoclonal Antibody (33.1), eBioscience™ (Product # 14-2029-82) and a 140 kDa band corresponding to TEK/TIE2 was observed across cell lines and tissues tested except U-87 MG and Mouse adipose which are reported to be negative. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), HUVEC (Lane 2), Mouse lung (Lane 3), Mouse kidney (Lane 4), Mouse adipose (Lane 5) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitroce... View More

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产品信息

14-2029-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

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产品规格

种属反应

Human, Mouse, Pig, Rabbit, Rat

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

33.1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_10870984

产品详细信息

Description: This 33.1 monoclonal antibody reacts with human, mouse, rabbit, rat, pig, and zebrafish CD202b. Also known as Tie-2, this molecule is a member of the tyrosine kinase receptor family. CD202b is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers-, c-Kit+, Sca1+ cells. Long-term multilineage repopulating cells were detected in Tie-2+, Lineage-, c-Kit+, Sca1+ cells but not in Tie-2-, Lineage-, c-Kit+, Sca1+ cells.

Applications Reported: This 33.1 antibody has been reported for use in western blotting.

Applications Tested: This 33.1 antibody has been tested by immunoblotting of reduced cell lysate prepared from the bEND3 cell line at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

PCSK9 attenuates efferocytosis in endothelial cells and promotes vascular aging.

Theranostics

Liu S,Wu J,Stolarz A,Zhang H,Boerma M,Byrum SD,Rusch NJ,Ding Z

14-2029-82 was used in Immunohistochemistry (Paraffin) to investigate the impact of PCSK9 on efferocytosis in endothelial cells (ECs) and its implications in vascular aging.

Fri Jun 09 00:00:00 UTC 2023

生物信息学

蛋白别名: Angiopoietin-1 receptor; CD202b; CD202b antigen; EC 2.7.10; EC 2.7.10.1; Endothelial tyrosine kinase; endothelial-specific receptor tyrosine kinase; hTIE2; HYK; mTIE2; p140 TEK; protein-tyrosine kinase Tie2; STK1; TEK tyrosine kinase, endothelial; TEKT1; Tektin-1; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; tyrosine kinases that contain immunoglobulin-like loops and epidermal growth factor-similar domains 2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2

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基因别名: AA517024; CD202B; Hyk; STK1; TEK; TIE-2; TIE2; VMCM; VMCM1

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UniProt ID: (Human) Q02763, (Mouse) Q02858