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CD204 Antibody (25-2045-42) in Flow
Human monocyte-derived macrophages were stained with Fixable Viability Dye eFluor® 506 (Product # 65-0866-14) and Fc-blocked with Human Fc Receptor Binding Inhibitor Purified (Product # 14-9161-73). Subsequently the cells were stained with Anti-Human CD11b APC (Product # 17-0118-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human CD204 PE-Cyanine7 (right). Total viable cells were used for analysis.
产品信息
25-2045-42
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
PSL204
偶联物
激发/发射光谱
569/780 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2662672
产品详细信息
This monoclonal antibody PSL204 reacts with human CD204, also known as Macrophage Scavenger Receptor 1 (MSR1), Scavenger Receptor Type A (SR-A), or SCARA1. It is expressed by multiple populations of macrophages and dendritic cells. CD204 belongs to the class A family of scavenger receptors, a group of five homotrimetric transmembrane proteins that contain a short cytoplasmic tail and extracellular C -terminus. Other members of this family include: MARCO (SCARA2), CSR (SCARA3), SRCL (SCARA4) and SCARA5. CD204 binds and internalizes oxidized low density lipoproteins (LDL), which drives macrophage differentiation into foam cells, secretion of inflammatory cytokines, and development of atherosclerotic lesions. In addition to oxidized LDL, CD204 binds and uptakes a wide variety of macromolecules, including bacterial cell wall components, viral DNA, calciprotein complexes, apoptotic cells, and beta-amyloid fibrils. This broad binding specificity implicates CD204-expressing macrophages in a number of physiological processes and pathologies, such as host defense, inflammation, bone remodeling, atherosclerosis, sepsis, and Alzheimer's disease.
In humans, CD204 exists as 3 splice variants. The more ubiquitous full length isoform (type I, SR-AI), a shorter isoform (type II, SR-AII) that lacks the C-terminal cysteine-rich domain, and the shortest isoform (type III, SR-AIII). Isoforms I and II bind modified LDL with similar affinity, whereas isoform III is not expressed on the cell surface. This monoclonal antibody PSL204 binds only the long (type I, SR-AI) isoform. The PSL204 will recognize CD204 on cells that have been formaldehyde-fixed and permeabilized. This antibody does not cross-react with mouse or rat CD204.
This PSL204 antibody has been pre-titrated and tested by flow cytometric analysis of human monocyte-derived macrophages. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD204 gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. Diseases associated with MSR1 include Barrett Esophagus and Prostate Cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD204; Macrophage acetylated LDL receptor I and II; macrophage scavenger receptor type III; Macrophage scavenger receptor types I and II; Scavenger receptor class A member 1; scavenger receptor class A, member 1; SR-AI/II
基因别名: CD204; MSR1; phSR1; phSR2; SCARA1; SR-A; SRA
UniProt ID: (Human) P21757
Entrez Gene ID: (Human) 4481
