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Invitrogen
CD208 (DC-LAMP) Monoclonal Antibody (31B), APC, eBioscience™
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CD208 (DC-LAMP) Antibody (17-2089-41) in Flow
In vitro differentiated human mature dendritic cells (7-day) were fixed and permeabilized and then stained with Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (blue histogram) or Anti-Human CD208 (DC-LAMP) APC (purple histogram). Total viable cells were used for analysis.
产品信息
17-2089-41
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
31B
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_11044500
产品详细信息
Description: This 31B monoclonal antibody reacts with human CD208, which is also known as dendritic cell lysosome-associated membrane glycoprotein (DC-LAMP). CD208 is expressed almost exclusively by mature dendritic cells. Expression of this molecule can be induced in monocytes by stimulation with GM-CSF and IL-4. Moreover, CD208 can be upregulated in CD34+-derived dendritic cells by GM-CSF, SCF, and TNFa. CD208 is also expressed in dendritic cells of non-lymphoid tissues such as skin. While CD208 can be detected on the cell surface, this protein is mainly localized within lysosomes. CD208 plays a key role in the processing and presentation of antigen during the immune response.
The 31B antibody only recognizes intracellular CD208, not the cell surface-expressed protein.
Applications Reported: This 31B antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 31B antibody has been pre-titrated and tested by flow cytometric analysis of in vitro differentiated mature dendritic cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD208; DC LAMP; DC-lysosome-associated membrane glycoprotein; LAMP-3; lysosomal-associated membrane protein 3; Lysosome-associated membrane glycoprotein 3; Protein TSC403
基因别名: CD208; DC LAMP; DC-LAMP; DCLAMP; LAMP; LAMP-3; LAMP3; TSC403
UniProt ID: (Human) Q9UQV4
Entrez Gene ID: (Human) 27074
adaptive immune response
regulation of autophagy
positive regulation of gene expression
response to interferon-alpha
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
viral entry into host cell
negative regulation of proteasomal protein catabolic process
regulation of viral life cycle
