Invitrogen

CD21 Monoclonal Antibody (2G9)

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CD21 Antibody (MA5-11417) in WB

Western blot analysis of CD21 was performed by loading 25 µg of Daudi (Lane 1), Raji (Lane 2), and BAF-3 cell lysates (Lane 3) and a molecular weight protein ladder onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4ºC overnight. The membrane was probed with a CD21 monoclonal antibody (Product # MA5-11417) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent det... View More

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产品信息

MA5-11417

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:500

免疫组化 (IHC)

查看9篇已发表文章 9篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:20

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1:2

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

2G9

抗原

Recombinant protein encoding the external domain of human CD21

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

tissue culture supernatant

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

AB_10982851

产品详细信息

MA5-11417 targets CD21 in WB, FACS and IHC (P) applications and shows reactivity with Human samples. This antibody is not suitable for Raji cell lysates or mouse BAF-3 cell lysates in Western blot analysis.

The MA5-11417 immunogen is recombinant protein encoding the external domain of human CD21.

靶标信息

CD21 (complement receptor 2, CR2, C3D receptor, EBV receptor) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to follicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands. CD21 functions as receptor for C3d, C3dg and iC3b complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation. Genetic variations in the CD21 gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively, spliced transcript variants encoding different isoforms of CD21 have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Single-cell profiling of immune cells after neoadjuvant pembrolizumab and chemotherapy in IIIA non-small cell lung cancer (NSCLC).

Cell death & disease

Hui Z,Zhang J,Ren Y,Li X,Yan C,Yu W,Wang T,Xiao S,Chen Y,Zhang R,Wei F,You J,Ren X

MA5-11417 was used in Immunohistochemistry to provide insights into mechanisms underlying anti-PD-1-based therapies and identified potential predictive factors and therapeutic targets for improving the efficiency of neoadjuvant chemoimmunotherapy in non-small cell lung cancer.

Wed Jul 13 00:00:00 UTC 2022

Epstein-Barr virus-associated adrenal smooth muscle tumors and disseminated diffuse large B-cell lymphoma in a child with common variable immunodeficiency: a case report and review of the literature.

International journal of surgical pathology

Petrilli G,Lorenzi L,Paracchini R,Ubiali A,Schumacher RF,Cabassa P,Facchetti F

MA5-11417 was used in immunohistochemistry to investigate the clinical and pathological features in a girl with common variable immunodeficiency

Mon Dec 01 00:00:00 UTC 2014

IgM expression in paraffin sections distinguishes follicular lymphoma from reactive follicular hyperplasia.

International journal of clinical and experimental pathology

Zheng Y,Zhou X,Xie J,Zhu H,Zhang S,Zhang Y,Wei X,Yue B

MA5-11417 was used in Immunohistochemistry on paraffin embedded tissues to suggest that a considerable loss of IgM expression in follicular dendritic cells is a typical feature of follicular lymphoma.

Tue May 26 00:00:00 UTC 2015

CXCR5⁺ T helper cells mediate protective immunity against tuberculosis.

The Journal of clinical investigation

Slight SR,Rangel-Moreno J,Gopal R,Lin Y,Fallert Junecko BA,Mehra S,Selman M,Becerril-Villanueva E,Baquera-Heredia J,Pavon L,Kaushal D,Reinhart TA,Randall TD,Khader SA

MA5-11417 was used in immunohistochemistry to study the role of CXCR5-positive T-cells in protective immunity against infection with Mycobacterium tuberculosis

Fri Feb 01 00:00:00 UTC 2013

Occurrence of nodular lymphocyte-predominant hodgkin lymphoma in hermansky-pudlak type 2 syndrome is associated to natural killer and natural killer T cell defects.

PloS one

Lorenzi L,Tabellini G,Vermi W,Moratto D,Porta F,Notarangelo LD,Patrizi O,Sozzani S,de Saint Basile G,Latour S,Pace D,Lonardi S,Facchetti F,Badolato R,Parolini S

MA5-11417 was used in immunohistochemistry to study factors associated with nodular lymphocyte-predominant hodgkin lymphoma in hermansky-pudlak type 2 syndrome

Wed Oct 01 00:00:00 UTC 2014

Folliculocentric B-cell-rich follicular dendritic cells sarcoma: a hitherto unreported morphological variant mimicking lymphoproliferative disorders.

Human pathology

Lorenzi L,Lonardi S,Petrilli G,Tanda F,Bella M,Laurino L,Rossi G,Facchetti F

MA5-11417 was used in immunohistochemistry to study three clinical cases of follicular dendritic cell sarcoma

Wed Feb 01 00:00:00 UTC 2012

The expression of IgM is helpful in the differentiation of primary cutaneous diffuse large B cell lymphoma and follicle center lymphoma.

Leukemia research

Demirkesen C,Tüzüner N,Esen T,Lebe B,Ozkal S

MA5-11417 was used in immunohistochemistry to investigate the effect of IgM expressoin on the differentiation of primary cutaneous diffuse large B cell lymphoma and follicle center lymphoma

Thu Sep 01 00:00:00 UTC 2011

Copy number alterations and expression profiles of candidate genes in a pulmonary inflammatory myofibroblastic tumor.

Lung cancer (Amsterdam, Netherlands)

Jung SH,Yim SH,Hu HJ,Jung CK,Lee SH,Kim DH,Chung YJ

MA5-11417 was used in immunohistochemistry to study the pathogenic mechanisms of pulmonary inflammatory myofibroblastic tumor

Mon Nov 01 00:00:00 UTC 2010

Diagnostic and differential diagnostic criteria of lymphoid neoplasms in bone marrow trephine biopsies: a study of 87 cases.

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie

Horváth E,Mezei T,Pávai Z,Turcu M,Demian S,Tóth E,Chira L,Jung I

MA5-11417 was used in immunohistochemistry to investigate the diagnosis of lymphoid neoplasmas through bone marrow trephine biopsies

Wed Dec 16 00:00:00 UTC 2009

Identification of CXCL13 as a new marker for follicular dendritic cell sarcoma.

The Journal of pathology

Vermi W,Lonardi S,Bosisio D,Uguccioni M,Danelon G,Pileri S,Fletcher C,Sozzani S,Zorzi F,Arrigoni G,Doglioni C,Ponzoni M,Facchetti F

MA5-11417 was used in immunohistochemistry to study the production CXCL13 by follicular dendritic cells and its utility as a marker of follicular dendritic cell sarcoma

Sat Nov 01 00:00:00 UTC 2008

生物信息学

蛋白别名: CD21; Complement C3d receptor; Complement C3d receptor (C3DR); complement component (3d/Epstein Barr virus) receptor 2; Complement receptor type 2; Complement Receptor type 2 (CR2); Cr2; EBV receptor; EBV-R; Epstein-Barr virus receptor; EVBR

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基因别名: C3DR; CD21; CR; CR2; CVID7; SLEB9

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UniProt ID: (Human) P20023

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Entrez Gene ID: (Human) 1380

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功能

virus receptor activity complement binding DNA binding complement receptor activity transmembrane signaling receptor activity protein homodimerization activity

参与通路

complement receptor mediated signaling pathway immune response complement activation, classical pathway B cell differentiation B cell proliferation innate immune response viral entry into host cell