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-Flow-20170216130641.jpg?time=20240412 "CD213a1 (IL-13Ra1) Antibody in Flow Cytometry (Flow)")
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CD213a1 (IL-13Ra1) Antibody (12-2130-80) in Flow
Splenocytes were cultured and stimulated with Anti-Mouse CD3 and Anti-Mouse CD28 for 3 days or harvested fresh on day 3 and then surface-stained with 0.25 µg of Anti-Mouse CD213a1 (IL-13Ra1) PE. The plot is an overlay of the stimulated cells (purple) and freshly isolated splenocytes (blue). Isotype control staining of the stimulated cells is indicated by the dotted line. Cells in the CD4+ lymphocyte gate we... View More
-Flow-20170216130641.jpg?time=20240412)
-Flow-20170216130641.jpg?time=20240412?time=20240412 "CD213a1 (IL-13Ra1) Antibody (12-2130-80) in Flow")
产品信息
12-2130-80
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
13MOKA
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10667742
产品详细信息
Description: The 13MOKA monoclonal antibody reacts with mouse CD213 alpha 1 (interleukin-13 receptor alpha 1, IL-13Ra1). CD213a1 is the low-affinity IL-13 receptor which mediates normal IL-13 inflammatory signaling. It has been shown that CD213a1 binds to IL-13 which then recruits CD124 (IL-4Ralpha) to stabilize the low-affinity interaction and form the active signaling complex. The CD213a1/CD124 complex can also serve as a receptor for IL-4, although the role of CD213a1 in this complex is poorly understood. CD213a1 is expressed on activated B and T cells and other cells.
Applications Reported: This 13MOKA antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 13MOKA antibody has been tested on mouse splenocytes stimulated for 3 days with Anti-CD3 and Anti-CD28 Functional Grade antibodies. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD antigen CD213a1; CD213a1; IL-13 receptor subunit alpha-1; IL-13RA1; Interleukin-13 receptor subunit alpha-1; Interleukin-13-binding protein; Novel cytokine receptor 4; NR4
基因别名: AI882074; CD213a1; IL-13R-alpha-1; IL-13r[a]; Il13r; Il13ra; Il13ra1; NR4
UniProt ID: (Mouse) O09030
Entrez Gene ID: (Mouse) 16164
