Invitrogen

CD221 (IGF1R) Monoclonal Antibody (1H7), APC, eBioscience™

CD221 (IGF1R) Antibody in Flow Cytometry (Flow)

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CD221 (IGF1R) Antibody (17-8849-42) in Flow

Staining of the HeLa cell line with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD221 (Insulin-like Growth Factor-1 Receptor) APC (purple histogram). Total viable cells were used for analysis.

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产品信息

17-8849-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

1H7

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1907364

产品详细信息

Description: This 1H7 monoclonal antibody reacts with human insulin-like growth factor-1 receptor (IGF-1R), also known as CD221. This receptor is a cell surface-expressed glycoprotein composed of two extracellular alpha subunits and two transmembrane beta subunits that possess tyrosine kinase activity. Expressed on nearly all cell types, this receptor binds IGF-I and IGF-II, as well as insulin. Ligand binding leads to activation of the PI3K/Akt and MAPK pathways, which mediate cell proliferation and survival. Many tumors and transformed cells display altered IGF-1R expression.

This monoclonal antibody has been reported to block binding of IGF-I and IGF-II to the IGF-1R.

Applications Reported: This 1H7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1H7 antibody has been pre-titrated and tested by flow cytometric analysis of the HeLa cell line. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IGF1R, also known as Insulin-like Growth Factor 1 (IGF-1) Receptor, is a receptor tyrosine kinase activated by IGF-1 and IGF-2. IGFR has been implicated in a variety of cancers. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Antibody targeting of E3 ubiquitin ligases for receptor degradation.

Nature

Marei H,Tsai WK,Kee YS,Ruiz K,He J,Cox C,Sun T,Penikalapati S,Dwivedi P,Choi M,Kan D,Saenz-Lopez P,Dorighi K,Zhang P,Kschonsak YT,Kljavin N,Amin D,Kim I,Mancini AG,Nguyen T,Wang C,Janezic E,Doan A,Mai E,Xi H,Gu C,Heinlein M,Biehs B,Wu J,Lehoux I,Harris S,Comps-Agrar L,Seshasayee D,de Sauvage FJ,Grimmer M,Li J,Agard NJ,de Sousa E Melo F

Published figure using CD221 (IGF1R) monoclonal antibody (Product # 17-8849-42) in Flow Cytometry

Sat Oct 01 00:00:00 UTC 2022

Effects of IGF-1 on Proliferation, Angiogenesis, Tumor Stem Cell Populations and Activation of AKT and Hedgehog Pathways in Oral Squamous Cell Carcinoma.

International journal of molecular sciences

Ferreira Mendes JM,de Faro Valverde L,Torres Andion Vidal M,Paredes BD,Coelho P,Allahdadi KJ,Coletta RD,Souza BSF,Rocha CAG

17-8849 was used in Flow cytometry/Cell sorting to suggest that IGF-1 exerts pro-tumorigenic effects on SCC-4 cells by stimulating proliferation, migration, invasion and stemness, as well as activating AKT and hedgehog pathways.

Sat Sep 05 00:00:00 UTC 2020

Effects of IGF-1 on Proliferation, Angiogenesis, Tumor Stem Cell Populations and Activation of AKT and Hedgehog Pathways in Oral Squamous Cell Carcinoma.

International journal of molecular sciences

Ferreira Mendes JM,de Faro Valverde L,Torres Andion Vidal M,Paredes BD,Coelho P,Allahdadi KJ,Coletta RD,Souza BSF,Rocha CAG

Sat Sep 05 00:00:00 UTC 2020

Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.

BMC cell biology

Kinzebach S,Dietz L,Klüter H,Thierse HJ,Bieback K

17-8849 was used in Flow cytometry/Cell sorting to study the functionally bioactive factors which are seen to affect mesenchymal stromal cells expansion.

Wed Oct 30 00:00:00 UTC 2013

生物信息学

蛋白别名: CD221; CD221 antigen; IGF 1 receptor; IGF 1R; IGF-I receptor; Insulin like growth factor 1 receptor precursor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2

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基因别名: CD221; IGF1R; IGFIR; IGFR; JTK13

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UniProt ID: (Human) P08069

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Entrez Gene ID: (Human) 3480

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功能