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CD226 (DNAM-1) Antibody (17-2261-82) in Flow
C57Bl/6 splenocytes were stimulated with Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82) and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) for 3 days and then stained with Anti-Mouse CD8a PE (Product # 12-0081-82) and 0.125 µg of Rat IgG2b K Isotype Control APC (Product # 17-4031-82) (left) or 0.125 µg of Anti-Mouse CD226 (DNAM-1) APC (right). Total viable cells were used for analysis.
产品信息
17-2261-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
10E5
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_11149875
产品详细信息
Description: This 10E5 monoclonal antibody reacts with mouse CD226, which is also known as DNAX accessory molecule-1 (DNAM-1). CD226 is an adhesion molecule expressed primarily on T cells, including thymocytes. The highest levels of CD226 are found on naive CD8 T cells. Moreover, expression of CD226 is regulated differentially in CD4 T cells. Under certain culture conditions, CD226 can be upregulated in Th1 and downregulated in Th2 and Th0 cells. This cell surface receptor can also be detected on natural killer cells, some B cell subsets, monocytes, and platelets. Interaction with its ligands, the poliovirus receptor (CD155) and CD112, results in adhesion and cell signaling events that promote innate and adaptive immunity, including differentiation, survival, and cytotoxicity.
The 10E5 monoclonal antibody has been reported to inhibit antigen-specific T cell proliferation and IFN-gamma production in vivo.
Applications Reported: This 10E5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 10E5 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
DNAM 1 is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes. DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells. DNAM-1 is also expressed by a subset of CD3 positive thymocytes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD226; CD226 antigen; DNAM 1; platelet and T cell activation antigen 1; Platelet and T-cell activation antigen 1
基因别名: BC051526; Cd226; DNAM-1; DNAM1; Pta1; TLiSA1
UniProt ID: (Mouse) Q8K4F0
Entrez Gene ID: (Mouse) 225825
cytokine production
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
positive regulation of immunoglobulin mediated immune response
cell adhesion
positive regulation of mast cell activation
positive regulation of natural killer cell mediated cytotoxicity
positive regulation of Fc receptor mediated stimulatory signaling pathway
